Restriction map for
pFN21AE5082
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# NO CUT ENZYMES ( 133 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI Alw44I AocI Aor51HI ApaLI
AscI AseI AsnI Asp718I AspEI
AspI AviII AvrII BalI BamHI
BanI BbeI BbrPI BcgI BciVI
BclI BcnI BfrI BlnI BsaAI
BsaBI BsaHI BsaI BseAI BsiEI
BsiWI Bsp1407I BspDI BspEI BspLU11I
BspMI BsrBI BsrFI BsrGI BssHII
Bst1107I BstAPI BstEII BstPI BstUI
BstZ17I Bsu36I Cfr10I ClaI CpoI
DraIII EaeI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRI EcoRV EcoT22I FauI FbaI
FseI FspI Hin1I HpaI KasI
KpnI KspI MamI MfeI MluI
MluNI MroI MscI MunI MvnI
NaeI NarI NciI NdeI NgoMIV
NheI NotI NruI NsiI PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PstI
PvuI RsrII SacII SalI SbfI
ScaI SexAI SfiI SfoI SgrAI
SmaI SmlI SnaBI Sse8387I SspBI
StuI SwaI Tth111I XbaI XcmI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 37 enzymes) #
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AciI 828 CCGC CCGC(-3/-1)
AlwNI 1460 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 1758 GGGCCC GGGCC^C
AspHI 1502 GAGCTC G[AT]GC[AT]^C
AvaI 938 CCCGAG C^[CT]CG[GA]G
BbvCI 502 CCTCAGC CCTCAGC(-5/-2)
BglI 643 GCCCAGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 299 AGATCT A^GATCT
BsaWI 1919 ACCGGA [AT]^CCGG[AT]
BsgI 1723 CTGCAC GTGCAG(16/14)
BsiHKAI 1502 GAGCTC G[AT]GC[AT]^C
BsmBI 969 GAGACG CGTCTC(1/5)
BsmI 231 GCATTC GAATGC(1/-1)
BsoBI 938 CCCGAG C^[CT]CG[GA]G
BspHI 584 TCATGA T^CATGA
BssSI 1970 CTCGTG CACGAG(-5/-1)
BstBI 1643 TTCGAA TT^CGAA
BstXI 680 CCAACATGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 299 AGATCT [GA]^GATC[CT]
DraI 979 TTTAAA TTT^AAA
DrdI 934 GACCCCCGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
HaeII 962 AGCGCC [GA]GCGC^[CT]
HapII 1920 CCGG C^CGG
HindIII 1594 AAGCTT A^AGCTT
HpaII 1920 CCGG C^CGG
MflI 299 AGATCT [GA]^GATC[CT]
MspI 1920 CCGG C^CGG
NspV 1643 TTCGAA TT^CGAA
PflMI 705 CCATGCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RcaI 584 TCATGA T^CATGA
SacI 1502 GAGCTC GAGCT^C
SapI 361 GAAGAGC GCTCTTC(1/4)
SfuI 1643 TTCGAA TT^CGAA
SpeI 1893 ACTAGT A^CTAGT
SspI 850 AATATT AAT^ATT
Van91I 705 CCATGCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 299 AGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AlwI 375 GATCC GGATC(4/5)
768 GATCC
Asp700I 535 GAACGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1462 GAAGCTGTTC
AvaII 63 GGTCC G^G[AT]CC
2010 GGACC
BlpI 477 GCTGAGC GC^T[ACGT]AGC
1015 GCTCAGC
Bpu1102I 477 GCTGAGC GC^T[ACGT]AGC
1015 GCTCAGC
BsmFI 1169 GGGAC GGGAC(10/14)
2009 GGGAC
CelII 477 GCTGAGC GC^T[ACGT]AGC
1015 GCTCAGC
DsaI 37 CCATGG C^C[GA][CT]GG
710 CCATGG
HgaI 971 GACGC GACGC(5/10)
1785 GCGTC
HincII 1282 GTTGAC GT[CT]^[GA]AC
1735 GTCAAC
HindII 1282 GTTGAC GT[CT]^[GA]AC
1735 GTCAAC
HphI 286 GGTGA GGTGA(8/7)
372 GGTGA
MaeII 1496 ACGT A^CGT
1739 ACGT
MslI 579 CACTTTCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1726 CACAAGGATG
NcoI 37 CCATGG C^CATGG
710 CCATGG
NspI 404 GCATGC [GA]CATG^[CT]
830 GCATGC
PpuMI 62 GGGTCCT [GA]G^G[AT]CC[CT]
2009 GGGACCT
SphI 404 GCATGC GCATG^C
830 GCATGC
Tsp45I 91 GTCAC ^GT[GC]AC
248 GTGAC
XmnI 535 GAACGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1462 GAAGCTGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 68 enzymes) #
#########################################
AcsI AfaI AluI ApoI BanII
BbsI BbvI BfaI BmyI BpmI
BpuAI BsaJI BseRI BsiYI BslI
BsmAI Bsp1286I BsrDI BsrI BssKI
BstF5I BstNI Cac8I CfoI Cfr13I
DdeI DpnII DraII EarI EcoO109I
EcoRII EcoT14I Fnu4HI FokI HaeIII
HhaI HinP1I HinfI ItaI Ksp632I
MaeI MaeIII MboI MboII MnlI
MseI MspA1I MvaI MwoI NdeII
NlaIII NlaIV PleI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
StyI TaqI TfiI Tru9I TseI
Tsp509I TspRI TthHB8I