Restriction map for
pFN21AE4310
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# NO CUT ENZYMES ( 137 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AflII AgeI Alw44I Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp700I Asp718I AspI AviII AvrII
BamHI BbeI BbrPI BbvCI BcgI
BciVI BclI BfaI BfrI BglI
BglII BlnI BlpI Bpu1102I BsaAI
BsaBI BsaI BsaWI BseAI BsgI
BsiEI BsiWI BsmBI BsmI Bsp1407I
BspEI BspHI BsrBI BsrDI BsrGI
BsrI BssHII Bst1107I BstBI BstEII
BstPI BstUI BstXI BstZ17I CelII
CpoI DraI DraIII DrdI EagI
EarI EclXI Eco47III Eco52I EcoO65I
EcoRI EcoT22I FauI FbaI FseI
FspI HincII HindII HindIII HpaI
KasI KpnI Ksp632I KspI MaeI
MamI MfeI MluI MroI MunI
MvnI NarI NdeI NheI NotI
NruI NsiI NspV PacI PflFI
PflMI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PstI PvuI
RcaI RsrII SacI SacII SalI
SapI SbfI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI Tth111I Van91I XbaI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AcsI 580 AAATTT [GA]^AATT[CT]
AflIII 842 ACATGT A^C[GA][CT]GT
AhdI 205 GACCTGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApoI 580 AAATTT [GA]^AATT[CT]
AspEI 205 GACCTGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 177 GAGCAC G[AT]GC[AT]^C
AvaI 827 CTCGAG C^[CT]CG[GA]G
BalI 2 TGGCCA TGG^CCA
BanII 91 GGGCTC G[GA]GC[CT]^C
BbsI 106 GAAGAC GAAGAC(2/6)
BpuAI 106 GAAGAC GAAGAC(2/6)
BsiHKAI 177 GAGCAC G[AT]GC[AT]^C
BsoBI 827 CTCGAG C^[CT]CG[GA]G
BspDI 774 ATCGAT AT^CGAT
BspLU11I 842 ACATGT A^CATGT
BspMI 237 GCAGGT ACCTGC(4/8)
BsrFI 310 GCCGGC [GA]^CCGG[CT]
BssSI 514 CTCGTG CACGAG(-5/-1)
Cfr10I 310 GCCGGC [GA]^CCGG[CT]
ClaI 774 ATCGAT AT^CGAT
DraII 533 AGGACCT [GA]G^G[ACGT]CC[CT]
EaeI 2 TGGCCA [CT]^GGCC[GA]
Eam1105I 205 GACCTGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO109I 533 AGGACCT [GA]G^G[ACGT]CC[CT]
HaeII 149 AGCGCC [GA]GCGC^[CT]
MaeII 278 ACGT A^CGT
MaeIII 908 GTGAC ^GT[ACGT]AC
MluNI 2 TGGCCA TGG^CCA
MscI 2 TGGCCA TGG^CCA
MseI 677 TTAA T^TAA
MslI 810 CATCAGAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 473 CAGCTG C[AC]G^C[GT]G
NaeI 310 GCCGGC GCC^GGC
NgoMIV 310 GCCGGC G^CCGGC
PaeR7I 827 CTCGAG C^TCGAG
PleI 872 GACTC GAGTC(4/5)
PpuMI 533 AGGACCT [GA]G^G[AT]CC[CT]
PvuII 473 CAGCTG CAG^CTG
ScaI 933 AGTACT AGT^ACT
SmlI 827 CTCGAG C^T[CT][GA]AG
TfiI 44 GATTC G^A[AT]TC
Tru9I 677 TTAA T^TAA
Tsp45I 908 GTGAC ^GT[GC]AC
TspRI 315 CACTG CA[GC]TG[ACGT][ACGT]^
XcmI 390 CCAGCAGCAATATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 827 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AciI 135 CCGC CCGC(-3/-1)
143 CCGC
AcyI 109 GACGCC G[GA]^CG[CT]C
258 GACGCC
AlwI 339 GATCC GGATC(4/5)
366 GATCC
AlwNI 67 CAGACCCTG CAG[ACGT][ACGT][ACGT]^CTG
532 CAGGACCTG
AocI 72 CCTGAGG CC^T[ACGT]AGG
358 CCTGAGG
AvaII 353 GGACC G^G[AT]CC
534 GGACC
BanI 97 GGCACC G^G[CT][GA]CC
790 GGCACC
BpmI 548 CTGGAG CTGGAG(16/14)
917 CTGGAG
BsaHI 109 GACGCC G[GA]^CG[CT]C
258 GACGCC
BseRI 361 GAGGAG GAGGAG(10/8)
953 GAGGAG
BsmFI 191 GGGAC GGGAC(10/14)
565 GGGAC
BstAPI 282 GCAAGAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
748 GCATATTTTGC
BstYI 338 AGATCC [GA]^GATC[CT]
365 AGATCC
Bsu36I 72 CCTGAGG CC^T[ACGT]AGG
358 CCTGAGG
Cac8I 310 GCCGGC GC[ACGT]^[ACGT]GC
714 GCTGGC
CfoI 150 GCGC GCG^C
372 GCGC
Eco81I 72 CCTGAGG CC^T[ACGT]AGG
358 CCTGAGG
EcoNI 72 CCTGAGGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
537 CCTGTATGAGG
EcoRV 648 GATATC GAT^ATC
850 GATATC
EcoT14I 5 CCATGG C^C[AT][AT]GG
53 CCATGG
HhaI 150 GCGC GCG^C
372 GCGC
Hin1I 109 GACGCC G[GA]^CG[CT]C
258 GACGCC
HinP1I 150 GCGC G^CGC
372 GCGC
HinfI 44 GATTC G^A[ACGT]TC
872 GACTC
MflI 338 AGATCC [GA]^GATC[CT]
365 AGATCC
NcoI 5 CCATGG C^CATGG
53 CCATGG
NspI 624 GCATGT [GA]CATG^[CT]
842 ACATGT
SfcI 719 CTATAG C^T[GA][CT]AG
924 CTACAG
StyI 5 CCATGG C^C[AT][AT]GG
53 CCATGG
XhoII 338 AGATCC [GA]^GATC[CT]
365 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 45 enzymes) #
#########################################
AfaI AluI BbvI BcnI BmyI
BsaJI BsiYI BslI BsmAI Bsp1286I
BssKI BstF5I BstNI Cfr13I DdeI
DpnII DsaI EcoRII Fnu4HI FokI
HaeIII HapII HgaI HpaII HphI
ItaI MboI MboII MnlI MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV RsaI Sau3AI Sau96I ScrFI
SfaNI TaqI TseI Tsp509I TthHB8I