Restriction map for
pFN21AE2194
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# NO CUT ENZYMES ( 173 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI Alw44I AlwNI AocI ApaI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspHI AspI AvaI
AvaII AviII AvrII BalI BamHI
BanI BanII BbeI BbrPI BbvCI
BclI BcnI BfrI BglII BlnI
BmyI BsaBI BsaHI BsaI BsaWI
BseAI BsgI BsiEI BsiHKAI BsiWI
BsiYI BslI BsmBI BsmI BsoBI
Bsp1286I Bsp1407I BspDI BspEI BspHI
BspLU11I BsrBI BsrFI BsrGI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstUI BstXI BstZ17I Bsu36I Cfr10I
Cfr13I ClaI CpoI DraI DraII
DraIII DrdI DsaI EagI Eam1105I
EclXI Eco52I Eco81I EcoNI EcoO109I
EcoO65I EcoRI EcoRV EcoT22I FauI
FbaI FseI FspI HapII HgaI
Hin1I HincII HindII HindIII HpaI
HpaII KasI KpnI KspI MamI
MluI MluNI MroI MscI MslI
MspA1I MspI MvnI NaeI NarI
NciI NcoI NdeI NgoMIV NheI
NlaIV NotI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI PvuII RcaI
RsrII SacI SacII SalI Sau96I
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI SspBI SspI StuI SwaI
Tth111I Van91I XbaI XcmI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AciI 433 GCGG CCGC(-3/-1)
AlwI 15 GGATC GGATC(4/5)
Aor51HI 735 AGCGCT AGC^GCT
BcgI 498 GCATCCTATTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 618 GGATAC GTATCC(6/5)
BglI 40 GCCGAGCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 49 GCTGAGC GC^T[ACGT]AGC
BpmI 177 CTGGAG CTGGAG(16/14)
Bpu1102I 49 GCTGAGC GC^T[ACGT]AGC
BsaAI 671 TACGTG [CT]AC^GT[GA]
BsaJI 350 CCAAGG C^C[ACGT][ACGT]GG
BseRI 221 GAGGAG GAGGAG(10/8)
BsmAI 268 GAGAC GTCTC(1/5)
BsmFI 377 GGGAC GGGAC(10/14)
BspMI 575 GCAGGT ACCTGC(4/8)
BsrDI 475 GCAATG GCAATG(2/0)
BstAPI 397 GCAGAATTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 15 GGATCT [GA]^GATC[CT]
CelII 49 GCTGAGC GC^T[ACGT]AGC
CfoI 736 GCGC GCG^C
EaeI 38 TGGCCG [CT]^GGCC[GA]
Eco47III 735 AGCGCT AGC^GCT
EcoT14I 350 CCAAGG C^C[AT][AT]GG
HaeII 735 AGCGCT [GA]GCGC^[CT]
HaeIII 39 GGCC GG^CC
HhaI 736 GCGC GCG^C
HinP1I 736 GCGC G^CGC
MfeI 605 CAATTG C^AATTG
MflI 15 GGATCT [GA]^GATC[CT]
MunI 605 CAATTG C^AATTG
NspI 701 ACATGC [GA]CATG^[CT]
SbfI 572 CCTGCAGG CCTGCA^GG
Sse8387I 572 CCTGCAGG CCTGCA^GG
StyI 350 CCAAGG C^C[AT][AT]GG
TaqI 9 TCGA T^CGA
TfiI 243 GATTC G^A[AT]TC
TspRI 339 CACTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 9 TCGA T^CGA
XhoII 15 GGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AcsI 400 GAATTT [GA]^AATT[CT]
548 AAATTC
AfaI 24 GTAC GT^AC
307 GTAC
ApoI 400 GAATTT [GA]^AATT[CT]
548 AAATTC
BbsI 226 GAAGAC GAAGAC(2/6)
751 GAAGAC
BpuAI 226 GAAGAC GAAGAC(2/6)
751 GAAGAC
BssKI 27 CCAGG ^CC[ACGT]GG
176 CCTGG
BstNI 27 CCAGG CC^[AT]GG
176 CCTGG
DpnII 7 GATC ^GATC
16 GATC
EarI 167 GAAGAG CTCTTC(1/4)
718 GAAGAG
EcoRII 27 CCAGG ^CC[AT]GG
176 CCTGG
HphI 709 GGTGA GGTGA(8/7)
715 GGTGA
Ksp632I 167 GAAGAG CTCTTC(1/4)
718 GAAGAG
MaeII 672 ACGT A^CGT
746 ACGT
MboI 7 GATC ^GATC
16 GATC
MseI 283 TTAA T^TAA
554 TTAA
MvaI 27 CCAGG CC^[AT]GG
176 CCTGG
NdeII 7 GATC ^GATC
16 GATC
NlaIII 660 CATG CATG^
702 CATG
PstI 573 CTGCAG CTGCA^G
739 CTGCAG
RsaI 24 GTAC GT^AC
307 GTAC
SapI 167 GAAGAGC GCTCTTC(1/4)
718 GAAGAGC
Sau3AI 7 GATC ^GATC
16 GATC
ScrFI 27 CCAGG CC^[ACGT]GG
176 CCTGG
SfaNI 601 GATGC GCATC(5/9)
498 GCATC
SfcI 573 CTGCAG C^T[GA][CT]AG
739 CTGCAG
Tru9I 283 TTAA T^TAA
554 TTAA
Tsp45I 293 GTGAC ^GT[GC]AC
710 GTGAC
#########################################
# 3 OR MORE CUT ENZYMES ( 19 enzymes) #
#########################################
AluI BbvI BfaI BsrI BstF5I
Cac8I DdeI Fnu4HI FokI HinfI
ItaI MaeI MaeIII MboII MnlI
MwoI PleI TseI Tsp509I