Restriction map for
pFN21AE4448
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# NO CUT ENZYMES ( 122 enzymes) #
##################################
AatII AccIII AclI AcsI AflII
AgeI AocI Aor51HI ApoI AscI
AseI AsnI Asp700I AspI AviII
AvrII BalI BbeI BbrPI BciVI
BclI BfrI BglII BlnI BlpI
Bpu1102I BsaAI BsaBI BsaI BsaWI
BseAI BsiEI BsiWI BsmBI Bsp1407I
BspEI BspHI BsrBI BsrDI BsrGI
BssHII BssSI BstAPI BstBI BstEII
BstPI Bsu36I CelII CpoI DraI
DraIII DrdI EagI EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoRI
EcoRV FauI FbaI FseI FspI
HincII HindII HindIII HpaI HphI
KasI KspI MamI MluI MluNI
MroI MscI MspA1I NaeI NarI
NdeI NgoMIV NheI NotI NruI
NspV PacI PflFI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PstI PvuI PvuII RcaI RsrII
SacI SacII SalI SapI SbfI
ScaI SfcI SfiI SfoI SfuI
SgrAI SmaI SnaBI Sse8387I SspBI
SspI SwaI Tth111I XbaI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AccI 1152 GTATAC GT^[AC][GT]AC
AccII 39 CGCG CG^CG
AcyI 34 GGCGTC G[GA]^CG[CT]C
Alw44I 694 GTGCAC G^TGCAC
ApaI 253 GGGCCC GGGCC^C
ApaLI 694 GTGCAC G^TGCAC
AspHI 694 GTGCAC G[AT]GC[AT]^C
BamHI 506 GGATCC G^GATCC
BbvCI 9 GCTGAGG CCTCAGC(-5/-2)
BcgI 967 CGAGGGGACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsaHI 34 GGCGTC G[GA]^CG[CT]C
BsgI 346 CTGCAC GTGCAG(16/14)
BsiHKAI 694 GTGCAC G[AT]GC[AT]^C
BsmAI 1089 GTCTC GTCTC(1/5)
BsmI 662 GAATGC GAATGC(1/-1)
BspDI 1259 ATCGAT AT^CGAT
BspLU11I 1325 ACATGT A^CATGT
BspMI 902 ACCTGC ACCTGC(4/8)
BsrFI 410 ACCGGC [GA]^CCGG[CT]
Bst1107I 1152 GTATAC GTA^TAC
BstUI 39 CGCG CG^CG
BstXI 1290 CCATCTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 1152 GTATAC GTA^TAC
Cfr10I 410 ACCGGC [GA]^CCGG[CT]
ClaI 1259 ATCGAT AT^CGAT
DsaI 1121 CCATGG C^C[GA][CT]GG
EaeI 682 CGGCCA [CT]^GGCC[GA]
EarI 755 CTCTTC CTCTTC(1/4)
EcoT22I 664 ATGCAT ATGCA^T
HaeII 6 GGCGCT [GA]GCGC^[CT]
HgaI 35 GCGTC GACGC(5/10)
Hin1I 34 GGCGTC G[GA]^CG[CT]C
Ksp632I 755 CTCTTC CTCTTC(1/4)
MaeII 534 ACGT A^CGT
MvnI 39 CGCG CG^CG
NcoI 1121 CCATGG C^CATGG
NsiI 664 ATGCAT ATGCA^T
PaeR7I 965 CTCGAG C^TCGAG
PflMI 546 CCACATCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SmlI 965 CTCGAG C^T[CT][GA]AG
SpeI 288 ACTAGT A^CTAGT
SphI 666 GCATGC GCATG^C
StuI 446 AGGCCT AGG^CCT
Van91I 546 CCACATCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 965 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
Acc65I 986 GGTACC G^GTACC
1354 GGTACC
AflIII 532 ACACGT A^C[GA][CT]GT
1325 ACATGT
AhdI 418 GACTATGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1306 GACAAGATGTC
AlwNI 46 CAGGTGCTG CAG[ACGT][ACGT][ACGT]^CTG
1015 CAGTCCCTG
Asp718I 986 GGTACC G^GTACC
1354 GGTACC
AspEI 418 GACTATGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1306 GACAAGATGTC
AvaI 494 CTCGGG C^[CT]CG[GA]G
965 CTCGAG
BanI 986 GGTACC G^G[CT][GA]CC
1354 GGTACC
BbsI 909 GAAGAC GAAGAC(2/6)
1113 GAAGAC
BcnI 30 CCGGG CC^[GC]GG
996 CCGGG
BglI 1195 GCCATTGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1288 GCCCATCTGGC
BpuAI 909 GAAGAC GAAGAC(2/6)
1113 GAAGAC
BsoBI 494 CTCGGG C^[CT]CG[GA]G
965 CTCGAG
BstYI 506 GGATCC [GA]^GATC[CT]
797 GGATCT
Cac8I 666 GCATGC GC[ACGT]^[ACGT]GC
1101 GCGAGC
Eam1105I 418 GACTATGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1306 GACAAGATGTC
KpnI 986 GGTACC GGTAC^C
1354 GGTACC
MfeI 224 CAATTG C^AATTG
815 CAATTG
MflI 506 GGATCC [GA]^GATC[CT]
797 GGATCT
MseI 851 TTAA T^TAA
1145 TTAA
MslI 222 CACAATTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
552 CATGGCTGTG
MunI 224 CAATTG C^AATTG
815 CAATTG
NciI 30 CCGGG CC^[GC]GG
996 CCGGG
PleI 68 GAGTC GAGTC(4/5)
1126 GAGTC
PpuMI 701 AGGACCT [GA]G^G[AT]CC[CT]
899 GGGACCT
SexAI 935 ACCAGGT A^CC[AT]GGT
1229 ACCTGGT
TaqI 966 TCGA T^CGA
1260 TCGA
TfiI 81 GATTC G^A[AT]TC
1134 GATTC
Tru9I 851 TTAA T^TAA
1145 TTAA
Tsp45I 559 GTGAC ^GT[GC]AC
1128 GTCAC
TthHB8I 966 TCGA T^CGA
1260 TCGA
XcmI 115 CCAGATGTGGAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
563 CCACACATGCCCTGG
XhoII 506 GGATCC [GA]^GATC[CT]
797 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 58 enzymes) #
#########################################
AciI AfaI AluI AlwI AvaII
BanII BbvI BfaI BmyI BpmI
BsaJI BseRI BsiYI BslI BsmFI
Bsp1286I BsrI BssKI BstF5I BstNI
CfoI Cfr13I DdeI DpnII DraII
EcoO109I EcoRII EcoT14I Fnu4HI FokI
HaeIII HapII HhaI HinP1I HinfI
HpaII ItaI MaeI MaeIII MboI
MboII MnlI MspI MvaI MwoI
NdeII NlaIII NlaIV NspI RsaI
Sau3AI Sau96I ScrFI SfaNI StyI
TseI Tsp509I TspRI