Restriction map for
pFN21AE6309
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# NO CUT ENZYMES ( 133 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AhdI
AocI Aor51HI ApaI AscI Asp718I
AspEI AspI AviII AvrII BalI
BamHI BanI BbeI BbsI BbvCI
BcgI BciVI BclI BfrI BglI
BlnI BlpI Bpu1102I BpuAI BsaBI
BsaHI BseAI BsiEI BsiWI BsmBI
Bsp1407I BspDI BspEI BspLU11I BsrBI
BsrDI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstUI BstZ17I Bsu36I
CelII ClaI CpoI DraI DraII
DraIII DrdI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO109I
EcoRI EcoRV EcoT22I FauI FbaI
FseI FspI HgaI Hin1I HindIII
HpaI KasI KpnI KspI MamI
MfeI MluI MluNI MroI MscI
MslI MspA1I MunI MvnI NaeI
NarI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PmeI PpuMI PshAI
Psp1406I PvuI PvuII RsrII SacII
SalI SapI SbfI SexAI SfiI
SfoI SfuI SgrAI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI Tth111I XhoI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AgeI 85 ACCGGT A^CCGGT
Alw44I 1075 GTGCAC G^TGCAC
AlwI 957 GGATC GGATC(4/5)
ApaLI 1075 GTGCAC G^TGCAC
AseI 289 ATTAAT AT^TAAT
AsnI 289 ATTAAT AT^TAAT
Asp700I 858 GAAATCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BbrPI 946 CACGTG CAC^GTG
BfaI 406 CTAG C^TAG
BglII 311 AGATCT A^GATCT
BpmI 130 CTGGAG CTGGAG(16/14)
BsaAI 946 CACGTG [CT]AC^GT[GA]
BsaWI 85 ACCGGT [AT]^CCGG[AT]
BseRI 641 GAGGAG GAGGAG(10/8)
BsmI 611 GCATTC GAATGC(1/-1)
BspMI 271 ACCTGC ACCTGC(4/8)
BstEII 527 GGTCACC G^GT[ACGT]ACC
BstPI 527 GGTCACC G^GT[ACGT]ACC
BstXI 65 CCAGGAACGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DsaI 150 CCATGG C^C[GA][CT]GG
EaeI 973 CGGCCA [CT]^GGCC[GA]
EcoO65I 527 GGTCACC G^GT[ACGT]ACC
HincII 379 GTTGAC GT[CT]^[GA]AC
HindII 379 GTTGAC GT[CT]^[GA]AC
MaeI 406 CTAG C^TAG
NcoI 150 CCATGG C^CATGG
PflMI 884 CCATTGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 85 ACCGGT A^CCGGT
PleI 630 GAGTC GAGTC(4/5)
PmaCI 946 CACGTG CAC^GTG
PmlI 946 CACGTG CAC^GTG
PshBI 289 ATTAAT AT^TAAT
PstI 994 CTGCAG CTGCA^G
SacI 1090 GAGCTC GAGCT^C
ScaI 965 AGTACT AGT^ACT
SfcI 994 CTGCAG C^T[GA][CT]AG
SmlI 961 CTCAAG C^T[CT][GA]AG
TfiI 210 GAATC G^A[AT]TC
Van91I 884 CCATTGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 405 TCTAGA T^CTAGA
XcmI 755 CCAAGGACTATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 858 GAAATCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AciI 165 GCGG CCGC(-3/-1)
373 GCGG
AcsI 344 AAATTT [GA]^AATT[CT]
847 GAATTT
AlwNI 267 CAGTACCTG CAG[ACGT][ACGT][ACGT]^CTG
1021 CAGAAGCTG
ApoI 344 AAATTT [GA]^AATT[CT]
847 GAATTT
AspHI 1075 GTGCAC G[AT]GC[AT]^C
1090 GAGCTC
AvaI 32 CCCGGG C^[CT]CG[GA]G
1040 CCCGGG
BbvI 541 GCAGC GCAGC(8/12)
20 GCTGC
BsaI 505 GAGACC GGTCTC(1/5)
931 GAGACC
BsgI 1005 CTGCAC GTGCAG(16/14)
484 GTGCAG
BsiHKAI 1075 GTGCAC G[AT]GC[AT]^C
1090 GAGCTC
BsmAI 505 GAGAC GTCTC(1/5)
931 GAGAC
BsmFI 44 GGGAC GGGAC(10/14)
632 GTCCC
BsoBI 32 CCCGGG C^[CT]CG[GA]G
1040 CCCGGG
BspHI 624 TCATGA T^CATGA
1094 TCATGA
BstF5I 102 GGATG GGATG(2/0)
729 GGATG
BstYI 311 AGATCT [GA]^GATC[CT]
957 GGATCT
CfoI 29 GCGC GCG^C
76 GCGC
EarI 616 CTCTTC CTCTTC(1/4)
638 GAAGAG
Fnu4HI 20 GCTGC GC^[ACGT]GC
541 GCAGC
FokI 102 GGATG GGATG(9/13)
729 GGATG
HaeII 28 AGCGCC [GA]GCGC^[CT]
75 GGCGCT
HhaI 29 GCGC GCG^C
76 GCGC
HinP1I 29 GCGC G^CGC
76 GCGC
HinfI 210 GAATC G^A[ACGT]TC
630 GAGTC
ItaI 20 GCTGC GC^[ACGT]GC
541 GCAGC
Ksp632I 616 CTCTTC CTCTTC(1/4)
638 GAAGAG
MflI 311 AGATCT [GA]^GATC[CT]
957 GGATCT
RcaI 624 TCATGA T^CATGA
1094 TCATGA
SmaI 32 CCCGGG CCC^GGG
1040 CCCGGG
TseI 20 GCTGC G^C[AT]GC
541 GCAGC
Tsp45I 528 GTCAC ^GT[GC]AC
949 GTGAC
XhoII 311 AGATCT [GA]^GATC[CT]
957 GGATCT
XmaCI 32 CCCGGG C^CCGGG
1040 CCCGGG
XmaI 32 CCCGGG C^CCGGG
1040 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 49 enzymes) #
#########################################
AfaI AluI AvaII BanII BcnI
BmyI BsaJI BsiYI BslI Bsp1286I
BsrFI BsrI BssKI BstNI Cac8I
Cfr10I Cfr13I DdeI DpnII EcoRII
EcoT14I HaeIII HapII HpaII HphI
MaeII MaeIII MboI MboII MnlI
MseI MspI MvaI MwoI NciI
NdeII NlaIII NlaIV RsaI Sau3AI
Sau96I ScrFI SfaNI StyI TaqI
Tru9I Tsp509I TspRI TthHB8I