Restriction map for
pFN21AE5123
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# NO CUT ENZYMES ( 194 enzymes) #
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AatII Acc65I AccI AccII AccIII
AciI AclI AcsI AcyI AflII
AflIII AgeI AhdI AluI Alw44I
AocI Aor51HI ApaI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AvaI AvaII AvrII
BalI BamHI BanI BanII BbeI
BbrPI BbsI BbvCI BbvI BcgI
BciVI BclI BfrI BglI BglII
BlnI BlpI Bpu1102I BpuAI BsaAI
BsaBI BsaHI BsaI BsaWI BseAI
BseRI BsgI BsiEI BsiWI BsiYI
BslI BsmAI BsmBI BsmFI BsoBI
BspDI BspEI BspHI BspLU11I BsrBI
BsrFI BssHII BssSI Bst1107I BstAPI
BstBI BstNI BstUI BstXI BstZ17I
Bsu36I Cac8I CelII Cfr10I Cfr13I
ClaI CpoI DdeI DraI DraII
DraIII DrdI DsaI EaeI EagI
Eam1105I EarI EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoRI EcoRII
EcoRV FauI FbaI Fnu4HI FseI
HaeII HgaI Hin1I HindIII HinfI
HpaI ItaI KasI KpnI Ksp632I
KspI MaeII MamI MfeI MluI
MluNI MroI MscI MspA1I MunI
MvaI MvnI NaeI NarI NcoI
NdeI NgoMIV NheI NlaIV NotI
NruI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PleI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI PvuII RcaI
RsrII SacI SacII SalI SapI
Sau96I SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I StuI
SwaI TaqI TfiI TseI Tsp45I
Tth111I TthHB8I Van91I XbaI XcmI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AfaI 397 GTAC GT^AC
AlwI 330 GGATC GGATC(4/5)
AlwNI 308 CAGAAACTG CAG[ACGT][ACGT][ACGT]^CTG
AviII 74 TGCGCA TGC^GCA
BcnI 35 CCGGG CC^[GC]GG
BfaI 355 CTAG C^TAG
BpmI 26 CTGGAG CTGGAG(16/14)
BsmI 278 GCATTC GAATGC(1/-1)
Bsp1407I 396 TGTACA T^GTACA
BspMI 385 GCAGGT ACCTGC(4/8)
BsrDI 418 GCAATG GCAATG(2/0)
BsrGI 396 TGTACA T^GTACA
BssKI 35 CCGGG ^CC[ACGT]GG
BstEII 289 GGTTACC G^GT[ACGT]ACC
BstF5I 237 GGATG GGATG(2/0)
BstPI 289 GGTTACC G^GT[ACGT]ACC
BstYI 330 GGATCT [GA]^GATC[CT]
CfoI 75 GCGC GCG^C
DpnII 331 GATC ^GATC
EcoO65I 289 GGTTACC G^GT[ACGT]ACC
EcoT14I 161 CCAAGG C^C[AT][AT]GG
EcoT22I 276 ATGCAT ATGCA^T
FokI 237 GGATG GGATG(9/13)
FspI 74 TGCGCA TGC^GCA
HaeIII 69 GGCC GG^CC
HapII 35 CCGG C^CGG
HhaI 75 GCGC GCG^C
HinP1I 75 GCGC G^CGC
HincII 129 GTCAAC GT[CT]^[GA]AC
HindII 129 GTCAAC GT[CT]^[GA]AC
HpaII 35 CCGG C^CGG
MaeI 355 CTAG C^TAG
MaeIII 290 GTTAC ^GT[ACGT]AC
MboI 331 GATC ^GATC
MflI 330 GGATCT [GA]^GATC[CT]
MslI 151 CATGGTCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 35 CCGG C^CGG
MwoI 77 GCAAAAACGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NciI 35 CCGGG CC^[GC]GG
NdeII 331 GATC ^GATC
NsiI 276 ATGCAT ATGCA^T
RsaI 397 GTAC GT^AC
Sau3AI 331 GATC ^GATC
ScrFI 35 CCGGG CC^[ACGT]GG
SfaNI 31 GATGC GCATC(5/9)
SfcI 437 CTGTAG C^T[GA][CT]AG
SspBI 396 TGTACA T^GTACA
SspI 247 AATATT AAT^ATT
StyI 161 CCAAGG C^C[AT][AT]GG
TspRI 61 CAGTG CA[GC]TG[ACGT][ACGT]^
XhoII 330 GGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 9 enzymes) #
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AspHI 63 GTGCTC G[AT]GC[AT]^C
94 GTGCTC
BmyI 63 GTGCTC G[AGT]GC[ACT]^C
94 GTGCTC
BsaJI 35 CCGGGG C^C[ACGT][ACGT]GG
161 CCAAGG
BsiHKAI 63 GTGCTC G[AT]GC[AT]^C
94 GTGCTC
Bsp1286I 63 GTGCTC G[AGT]GC[ACT]^C
94 GTGCTC
MnlI 328 GAGG CCTC(7/6)
370 GAGG
MseI 251 TTAA T^TAA
335 TTAA
Tru9I 251 TTAA T^TAA
335 TTAA
Tsp509I 12 AATT ^AATT
180 AATT
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# 3 OR MORE CUT ENZYMES ( 4 enzymes) #
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BsrI HphI MboII NlaIII