Restriction map for
pFN21AE6148
##################################
# NO CUT ENZYMES ( 185 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AflIII AgeI
AhdI Alw44I AlwNI AocI Aor51HI
ApaI ApaLI ApoI AscI AseI
AsnI Asp718I AspEI AspI AvaII
AviII AvrII BamHI BanI BbeI
BbrPI BbvCI BciVI BclI BcnI
BfaI BglI BglII BlnI BlpI
BpmI Bpu1102I BsaAI BsaBI BsaHI
BsaI BsaJI BsaWI BseAI BsgI
BsiEI BsiWI BsmI Bsp1407I BspDI
BspEI BspLU11I BsrBI BsrDI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstXI
BstZ17I Bsu36I Cac8I CelII CfoI
Cfr10I Cfr13I ClaI CpoI DdeI
DraI DraII DraIII DrdI DsaI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoRI
EcoRV EcoT14I EcoT22I FauI FbaI
FseI FspI HaeII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HindIII HpaI HpaII KasI KpnI
KspI MaeI MaeII MamI MfeI
MluI MroI MslI MspA1I MspI
MunI MvnI NaeI NarI NciI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI PvuII RsrII SacII
SalI Sau96I SbfI ScaI SexAI
SfaNI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI StyI SwaI
TaqI Tsp509I TspRI Tth111I TthHB8I
Van91I XbaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AfaI 252 GTAC GT^AC
AflII 10 CTTAAG C^TTAAG
Asp700I 45 GAAGGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 277 GAGCTC G[AT]GC[AT]^C
AvaI 282 CCCGAG C^[CT]CG[GA]G
BbvI 159 GCAGC GCAGC(8/12)
BcgI 201 GCAGGAGCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 10 CTTAAG C^TTAAG
BseRI 300 CTCCTC GAGGAG(10/8)
BsiHKAI 277 GAGCTC G[AT]GC[AT]^C
BsmBI 93 CGTCTC CGTCTC(1/5)
BsmFI 257 GGGAC GGGAC(10/14)
BsoBI 282 CCCGAG C^[CT]CG[GA]G
BspHI 273 TCATGA T^CATGA
BsrI 128 ACTGG ACTGG(1/-1)
BssKI 243 CCTGG ^CC[ACGT]GG
BstF5I 131 GGATG GGATG(2/0)
BstNI 243 CCTGG CC^[AT]GG
BstYI 24 GGATCT [GA]^GATC[CT]
EarI 5 CTCTTC CTCTTC(1/4)
EcoRII 243 CCTGG ^CC[AT]GG
Fnu4HI 159 GCAGC GC^[ACGT]GC
FokI 131 GGATG GGATG(9/13)
ItaI 159 GCAGC GC^[ACGT]GC
Ksp632I 5 CTCTTC CTCTTC(1/4)
MflI 24 GGATCT [GA]^GATC[CT]
MseI 11 TTAA T^TAA
MvaI 243 CCTGG CC^[AT]GG
NlaIV 174 GGAGCC GG[ACGT]^[ACGT]CC
PleI 285 GAGTC GAGTC(4/5)
PstI 199 CTGCAG CTGCA^G
RcaI 273 TCATGA T^CATGA
RsaI 252 GTAC GT^AC
SacI 277 GAGCTC GAGCT^C
SapI 4 GCTCTTC GCTCTTC(1/4)
ScrFI 243 CCTGG CC^[ACGT]GG
SfcI 199 CTGCAG C^T[GA][CT]AG
SmlI 10 CTTAAG C^T[CT][GA]AG
TfiI 66 GAATC G^A[AT]TC
Tru9I 11 TTAA T^TAA
TseI 159 GCAGC G^C[AT]GC
XcmI 233 CCAACCCTTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 24 GGATCT [GA]^GATC[CT]
XmnI 45 GAAGGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AciI 35 CCGC CCGC(-3/-1)
295 CCGC
AlwI 85 GATCC GGATC(4/5)
24 GGATC
BalI 230 TGGCCA TGG^CCA
245 TGGCCA
BanII 175 GAGCCC G[GA]GC[CT]^C
277 GAGCTC
BbsI 103 GAAGAC GAAGAC(2/6)
76 GTCTTC
BmyI 175 GAGCCC G[AGT]GC[ACT]^C
277 GAGCTC
BpuAI 103 GAAGAC GAAGAC(2/6)
76 GTCTTC
BsiYI 237 CCCTTTCCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
248 CCAAGTACAGG
BslI 237 CCCTTTCCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
248 CCAAGTACAGG
Bsp1286I 175 GAGCCC G[AGT]GC[ACT]^C
277 GAGCTC
BspMI 72 GCAGGT ACCTGC(4/8)
162 GCAGGT
DpnII 25 GATC ^GATC
85 GATC
EaeI 230 TGGCCA [CT]^GGCC[GA]
245 TGGCCA
HinfI 66 GAATC G^A[ACGT]TC
285 GAGTC
MboI 25 GATC ^GATC
85 GATC
MluNI 230 TGGCCA TGG^CCA
245 TGGCCA
MscI 230 TGGCCA TGG^CCA
245 TGGCCA
MwoI 63 GCTGAATCAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
177 GCCCCAAGAGC
NdeII 25 GATC ^GATC
85 GATC
NlaIII 108 CATG CATG^
274 CATG
Sau3AI 25 GATC ^GATC
85 GATC
#########################################
# 3 OR MORE CUT ENZYMES ( 8 enzymes) #
#########################################
AluI BsmAI HaeIII HphI MaeIII
MboII MnlI Tsp45I