Restriction map for
pFN21AE4528
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# NO CUT ENZYMES ( 93 enzymes) #
##################################
AatII AccIII AclI AcsI AflII
AgeI AhdI Alw44I AocI ApaI
ApaLI ApoI AscI AseI AsnI
Asp700I AspEI AspI AvrII BbrPI
BbvCI BcgI BclI BfrI BglII
BlnI BsaBI BsaWI BseAI BseRI
BsmI BspDI BspEI BsrDI Bst1107I
BstAPI BstBI BstEII BstPI BstZ17I
Bsu36I ClaI DraI EagI Eam1105I
EclXI Eco52I Eco81I EcoNI EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
HindIII HpaI MamI MfeI MluI
MroI MunI NdeI NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuII SapI
SbfI ScaI SfuI SnaBI SpeI
Sse8387I SspI SwaI Tsp509I Tth111I
XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 43 enzymes) #
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Acc65I 988 GGTACC G^GTACC
AccI 430 GTCGAC GT^[AC][GT]AC
AlwNI 334 CAGTGCCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 626 AGCGCT AGC^GCT
Asp718I 988 GGTACC G^GTACC
AviII 1364 TGCGCA TGC^GCA
BanII 742 GAGCTC G[GA]GC[CT]^C
BbsI 448 GTCTTC GAAGAC(2/6)
BciVI 901 GTATCC GTATCC(6/5)
BpuAI 448 GTCTTC GAAGAC(2/6)
BsaI 1215 GGTCTC GGTCTC(1/5)
BsiEI 141 CGATCG CG[GA][CT]^CG
BsiWI 473 CGTACG C^GTACG
BsmBI 1087 GAGACG CGTCTC(1/5)
BspHI 101 TCATGA T^CATGA
BstXI 1421 CCAAGTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CpoI 1705 CGGACCG CG^G[AT]CCG
DraIII 193 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
EarI 211 CTCTTC CTCTTC(1/4)
Eco47III 626 AGCGCT AGC^GCT
FspI 1364 TGCGCA TGC^GCA
KpnI 988 GGTACC GGTAC^C
Ksp632I 211 CTCTTC CTCTTC(1/4)
MseI 806 TTAA T^TAA
NcoI 728 CCATGG C^CATGG
PflMI 333 CCAGTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 29 CTGCAG CTGCA^G
PvuI 141 CGATCG CGAT^CG
RcaI 101 TCATGA T^CATGA
RsrII 1705 CGGACCG CG^G[AT]CCG
SacI 742 GAGCTC GAGCT^C
SalI 430 GTCGAC G^TCGAC
SexAI 194 ACCTGGT A^CC[AT]GGT
SfcI 29 CTGCAG C^T[GA][CT]AG
SgrAI 229 CACCGGCG C[GA]^CCGG[CT]G
SmaI 770 CCCGGG CCC^GGG
SmlI 958 CTTGAG C^T[CT][GA]AG
SphI 1031 GCATGC GCATG^C
TfiI 679 GATTC G^A[AT]TC
Tru9I 806 TTAA T^TAA
Van91I 333 CCAGTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 770 CCCGGG C^CCGGG
XmaI 770 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 39 enzymes) #
###################################
AspHI 226 GAGCAC G[AT]GC[AT]^C
742 GAGCTC
AvaI 770 CCCGGG C^[CT]CG[GA]G
1467 CTCGGG
BalI 1325 TGGCCA TGG^CCA
1655 TGGCCA
BamHI 564 GGATCC G^GATCC
800 GGATCC
BbeI 532 GGCGCC GGCGC^C
1633 GGCGCC
BfaI 922 CTAG C^TAG
1435 CTAG
BlpI 1035 GCTCAGC GC^T[ACGT]AGC
1113 GCTGAGC
Bpu1102I 1035 GCTCAGC GC^T[ACGT]AGC
1113 GCTGAGC
BsgI 694 GTGCAG GTGCAG(16/14)
1309 GTGCAG
BsiHKAI 226 GAGCAC G[AT]GC[AT]^C
742 GAGCTC
BsoBI 770 CCCGGG C^[CT]CG[GA]G
1467 CTCGGG
Bsp1407I 653 TGTACA T^GTACA
1160 TGTACA
BspLU11I 434 ACATGT A^CATGT
1400 ACATGT
BspMI 326 ACCTGC ACCTGC(4/8)
696 GCAGGT
BsrBI 871 GAGCGG CCGCTC(-3/-3)
1584 GAGCGG
BsrGI 653 TGTACA T^GTACA
1160 TGTACA
BssSI 1564 CACGAG CACGAG(-5/-1)
745 CTCGTG
BstYI 564 GGATCC [GA]^GATC[CT]
800 GGATCC
CelII 1035 GCTCAGC GC^T[ACGT]AGC
1113 GCTGAGC
HincII 430 GTCGAC GT[CT]^[GA]AC
1662 GTTGAC
HindII 430 GTCGAC GT[CT]^[GA]AC
1662 GTTGAC
KasI 532 GGCGCC G^GCGCC
1633 GGCGCC
KspI 512 CCGCGG CCGC^GG
1622 CCGCGG
MaeI 922 CTAG C^TAG
1435 CTAG
MaeIII 704 GTGAC ^GT[ACGT]AC
1438 GTCAC
MflI 564 GGATCC [GA]^GATC[CT]
800 GGATCC
MluNI 1325 TGGCCA TGG^CCA
1655 TGGCCA
MscI 1325 TGGCCA TGG^CCA
1655 TGGCCA
MslI 390 CATGGTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
471 CACGTACGTG
NarI 532 GGCGCC GG^CGCC
1633 GGCGCC
SacII 512 CCGCGG CCGC^GG
1622 CCGCGG
SfaNI 265 GATGC GCATC(5/9)
908 GCATC
SfoI 532 GGCGCC GGC^GCC
1633 GGCGCC
SspBI 653 TGTACA T^GTACA
1160 TGTACA
TaqI 431 TCGA T^CGA
682 TCGA
Tsp45I 704 GTGAC ^GT[GC]AC
1438 GTCAC
TthHB8I 431 TCGA T^CGA
682 TCGA
XcmI 728 CCATGGCACTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
993 CCACTGTACTTTTGG
XhoII 564 GGATCC [GA]^GATC[CT]
800 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 83 enzymes) #
#########################################
AccII AciI AcyI AfaI AflIII
AluI AlwI AvaII BanI BbvI
BcnI BglI BmyI BpmI BsaAI
BsaHI BsaJI BsiYI BslI BsmAI
BsmFI Bsp1286I BsrFI BsrI BssHII
BssKI BstF5I BstNI BstUI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DrdI DsaI EaeI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI MaeII MboI MboII MnlI
MspA1I MspI MvaI MvnI MwoI
NaeI NciI NdeII NgoMIV NlaIII
NlaIV NspI PleI PpuMI RsaI
Sau3AI Sau96I ScrFI SfiI StuI
StyI TseI TspRI