Restriction map for
pFN21AE1465
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# NO CUT ENZYMES ( 138 enzymes) #
##################################
Acc65I AccIII AclI AcsI AflII
AflIII AgeI AhdI Alw44I AocI
Aor51HI ApaI ApaLI ApoI AscI
AseI AsnI Asp718I AspEI AspI
AvaI AviII AvrII BalI BamHI
BbrPI BcgI BciVI BclI BfrI
BlnI BlpI Bpu1102I BsaAI BsaBI
BseAI BseRI BsiEI BsiWI BsmBI
BsmI BsoBI BspDI BspEI BspHI
BspLU11I BsrBI BsrDI BsrFI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstXI BstZ17I Bsu36I CelII Cfr10I
ClaI CpoI DraI DraIII DrdI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI FspI HindIII HinfI
HpaI KpnI KspI MamI MfeI
MluI MluNI MroI MscI MseI
MunI NaeI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PinAI
PleI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacII SalI SbfI ScaI SexAI
SfiI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspI StuI
SwaI TfiI Tru9I Tth111I XbaI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 36 enzymes) #
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AatII 1012 GACGTC GACGT^C
AccI 766 GTCTAC GT^[AC][GT]AC
Asp700I 444 GAAGGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BbeI 798 GGCGCC GGCGC^C
BbsI 448 GTCTTC GAAGAC(2/6)
BfaI 892 CTAG C^TAG
BglI 714 GCCTGATGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 560 AGATCT A^GATCT
BpuAI 448 GTCTTC GAAGAC(2/6)
BsaI 142 GAGACC GGTCTC(1/5)
BsaWI 665 TCCGGT [AT]^CCGG[AT]
BsgI 999 CTGCAC GTGCAG(16/14)
BsmAI 142 GAGAC GTCTC(1/5)
Bsp1407I 746 TGTACA T^GTACA
BsrGI 746 TGTACA T^GTACA
EaeI 263 CGGCCA [CT]^GGCC[GA]
EarI 34 CTCTTC CTCTTC(1/4)
EcoT14I 960 CCAAGG C^C[AT][AT]GG
HincII 931 GTCAAC GT[CT]^[GA]AC
HindII 931 GTCAAC GT[CT]^[GA]AC
KasI 798 GGCGCC G^GCGCC
Ksp632I 34 CTCTTC CTCTTC(1/4)
MaeI 892 CTAG C^TAG
MaeII 1013 ACGT A^CGT
NarI 798 GGCGCC GG^CGCC
PflMI 636 CCAGGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 569 GGGTCCC [GA]G^G[AT]CC[CT]
SacI 538 GAGCTC GAGCT^C
SapI 33 GCTCTTC GCTCTTC(1/4)
SfaNI 507 GATGC GCATC(5/9)
SfoI 798 GGCGCC GGC^GCC
SspBI 746 TGTACA T^GTACA
StyI 960 CCAAGG C^C[AT][AT]GG
Tsp509I 871 AATT ^AATT
Van91I 636 CCAGGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 444 GAAGGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
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AccII 220 CGCG CG^CG
1089 CGCG
AlwNI 993 CAGCTTCTG CAG[ACGT][ACGT][ACGT]^CTG
1036 CAGGTGCTG
AspHI 538 GAGCTC G[AT]GC[AT]^C
754 GAGCAC
BanII 31 GGGCTC G[GA]GC[CT]^C
538 GAGCTC
BbvCI 728 CCTCAGC CCTCAGC(-5/-2)
459 GCTGAGG
BsiHKAI 538 GAGCTC G[AT]GC[AT]^C
754 GAGCAC
BspMI 296 ACCTGC ACCTGC(4/8)
1035 GCAGGT
BstAPI 992 GCAGCTTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1035 GCAGGTGCTGC
BstUI 220 CGCG CG^CG
1089 CGCG
DraII 569 GGGTCCC [GA]G^G[ACGT]CC[CT]
659 GGGGCCT
EcoNI 42 CCTCTCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
702 CCTTCGGAAGG
EcoO109I 569 GGGTCCC [GA]G^G[ACGT]CC[CT]
659 GGGGCCT
FauI 218 CCCGC CCCGC(4/6)
314 CCCGC
HaeII 798 GGCGCC [GA]GCGC^[CT]
1072 AGCGCC
HgaI 1087 GACGC GACGC(5/10)
368 GCGTC
HphI 350 TCACC GGTGA(8/7)
422 TCACC
MaeIII 275 GTGAC ^GT[ACGT]AC
669 GTCAC
MspA1I 742 CAGCTG C[AC]G^C[GT]G
910 CAGCTG
MvnI 220 CGCG CG^CG
1089 CGCG
PstI 913 CTGCAG CTGCA^G
1033 CTGCAG
PvuII 742 CAGCTG CAG^CTG
910 CAGCTG
SfcI 913 CTGCAG C^T[GA][CT]AG
1033 CTGCAG
Tsp45I 275 GTGAC ^GT[GC]AC
669 GTCAC
#########################################
# 3 OR MORE CUT ENZYMES ( 61 enzymes) #
#########################################
AciI AcyI AfaI AluI AlwI
AvaII BanI BbvI BcnI BmyI
BpmI BsaHI BsaJI BsiYI BslI
BsmFI Bsp1286I BsrI BssKI BstF5I
BstNI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DsaI EcoRII Fnu4HI
FokI HaeIII HapII HhaI Hin1I
HinP1I HpaII ItaI MboI MboII
MflI MnlI MslI MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
RsaI Sau3AI Sau96I ScrFI SmlI
TaqI TseI TspRI TthHB8I XcmI
XhoII