Restriction map for
pFN21AE5710
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# NO CUT ENZYMES ( 95 enzymes) #
##################################
AatII Acc65I AccI AccIII AflIII
AgeI Alw44I Aor51HI ApaLI AscI
AseI AsnI Asp700I Asp718I AspI
AvrII BbrPI BbvCI BcgI BciVI
BclI BcnI BlnI BsaAI BsaBI
BsaI BsaWI BseAI BsiWI BsmI
BspEI BspHI BspLU11I BssHII Bst1107I
BstBI BstEII BstPI BstZ17I CpoI
DraI DraIII DrdI Eco47III EcoO65I
EcoRI FbaI FseI HincII HindII
HindIII HpaI KpnI MamI MluI
MroI NciI NdeI NheI NotI
NruI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PshAI PshBI PvuI RcaI RsrII
SalI SbfI ScaI SexAI SfuI
SgrAI SmaI SnaBI SpeI Sse8387I
SspI StuI SwaI Tth111I Van91I
XbaI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AclI 117 AACGTT AA^CGTT
AcsI 574 AAATTC [GA]^AATT[CT]
AflII 301 CTTAAG C^TTAAG
AhdI 2320 GACATAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 1220 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 1971 GGGCCC GGGCC^C
ApoI 574 AAATTC [GA]^AATT[CT]
AspEI 2320 GACATAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 2102 TGCGCA TGC^GCA
BalI 1385 TGGCCA TGG^CCA
BamHI 1298 GGATCC G^GATCC
BanI 9 GGCGCC G^G[CT][GA]CC
BbeI 9 GGCGCC GGCGC^C
BfrI 301 CTTAAG C^TTAAG
BglII 2216 AGATCT A^GATCT
BsiEI 984 CGGCCG CG[GA][CT]^CG
Bsp1407I 971 TGTACA T^GTACA
BspDI 2458 ATCGAT AT^CGAT
BsrDI 1044 CATTGC GCAATG(2/0)
BsrGI 971 TGTACA T^GTACA
BssSI 60 CACGAG CACGAG(-5/-1)
BstAPI 826 GCAGCCAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
ClaI 2458 ATCGAT AT^CGAT
EagI 984 CGGCCG C^GGCCG
Eam1105I 2320 GACATAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 984 CGGCCG C^GGCCG
Eco52I 984 CGGCCG C^GGCCG
EcoNI 402 CCTGATTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 1549 GATATC GAT^ATC
EcoT22I 899 ATGCAT ATGCA^T
FspI 2102 TGCGCA TGC^GCA
KasI 9 GGCGCC G^GCGCC
KspI 360 CCGCGG CCGC^GG
MaeII 118 ACGT A^CGT
MfeI 2415 CAATTG C^AATTG
MluNI 1385 TGGCCA TGG^CCA
MscI 1385 TGGCCA TGG^CCA
MunI 2415 CAATTG C^AATTG
NarI 9 GGCGCC GG^CGCC
NcoI 2034 CCATGG C^CATGG
NsiI 899 ATGCAT ATGCA^T
NspI 1370 GCATGC [GA]CATG^[CT]
PpuMI 1005 AGGACCC [GA]G^G[AT]CC[CT]
Psp1406I 117 AACGTT AA^CGTT
SacII 360 CCGCGG CCGC^GG
SapI 127 GAAGAGC GCTCTTC(1/4)
SfiI 2179 GGCCGCTTCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 9 GGCGCC GGC^GCC
SphI 1370 GCATGC GCATG^C
SspBI 971 TGTACA T^GTACA
XcmI 1433 CCACCAACCACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 35 enzymes) #
###################################
AccII 361 CGCG CG^CG
2246 CGCG
AcyI 9 GGCGCC G[GA]^CG[CT]C
2115 GACGCC
AocI 496 CCTGAGG CC^T[ACGT]AGG
1813 CCTAAGG
AvaI 34 CCCGAG C^[CT]CG[GA]G
2528 CCCGAG
AvaII 1006 GGACC G^G[AT]CC
1446 GGACC
BbsI 1494 GAAGAC GAAGAC(2/6)
2473 GAAGAC
BfaI 1234 CTAG C^TAG
2341 CTAG
BlpI 559 GCTAAGC GC^T[ACGT]AGC
2260 GCTGAGC
Bpu1102I 559 GCTAAGC GC^T[ACGT]AGC
2260 GCTGAGC
BpuAI 1494 GAAGAC GAAGAC(2/6)
2473 GAAGAC
BsaHI 9 GGCGCC G[GA]^CG[CT]C
2115 GACGCC
BsgI 512 CTGCAC GTGCAG(16/14)
1542 CTGCAC
BsmBI 1509 CGTCTC CGTCTC(1/5)
2113 GAGACG
BsoBI 34 CCCGAG C^[CT]CG[GA]G
2528 CCCGAG
BspMI 2129 ACCTGC ACCTGC(4/8)
2147 ACCTGC
BsrBI 935 CCGCTC CCGCTC(-3/-3)
1180 GAGCGG
BstUI 361 CGCG CG^CG
2246 CGCG
BstYI 1298 GGATCC [GA]^GATC[CT]
2216 AGATCT
Bsu36I 496 CCTGAGG CC^T[ACGT]AGG
1813 CCTAAGG
CelII 559 GCTAAGC GC^T[ACGT]AGC
2260 GCTGAGC
DsaI 360 CCGCGG C^C[GA][CT]GG
2034 CCATGG
EaeI 984 CGGCCG [CT]^GGCC[GA]
1385 TGGCCA
Eco81I 496 CCTGAGG CC^T[ACGT]AGG
1813 CCTAAGG
EcoT14I 377 CCAAGG C^C[AT][AT]GG
2034 CCATGG
HgaI 2115 GACGC GACGC(5/10)
297 GCGTC
Hin1I 9 GGCGCC G[GA]^CG[CT]C
2115 GACGCC
MaeI 1234 CTAG C^TAG
2341 CTAG
MflI 1298 GGATCC [GA]^GATC[CT]
2216 AGATCT
MvnI 361 CGCG CG^CG
2246 CGCG
NaeI 982 GCCGGC GCC^GGC
2522 GCCGGC
NgoMIV 982 GCCGGC G^CCGGC
2522 GCCGGC
PstI 1277 CTGCAG CTGCA^G
2131 CTGCAG
SacI 286 GAGCTC GAGCT^C
1798 GAGCTC
StyI 377 CCAAGG C^C[AT][AT]GG
2034 CCATGG
XhoII 1298 GGATCC [GA]^GATC[CT]
2216 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 77 enzymes) #
#########################################
AciI AfaI AluI AlwI AspHI
BanII BbvI BglI BmyI BpmI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BsrFI BsrI
BssKI BstF5I BstNI BstXI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII EarI EcoO109I EcoRII FauI
Fnu4HI FokI HaeII HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeIII MboI MboII
MnlI MseI MslI MspA1I MspI
MvaI MwoI NdeII NlaIII NlaIV
PleI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I