Restriction map for
pFN21AE5615
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# NO CUT ENZYMES ( 133 enzymes) #
##################################
AatII AccII AccIII AclI AcyI
AflIII AgeI AhdI AlwNI AocI
Aor51HI ApaI AscI AspEI AspI
AviII AvrII BamHI BanII BbeI
BbrPI BbvCI BciVI BclI BglI
BglII BlnI BlpI Bpu1102I BsaAI
BsaHI BsaWI BseAI BseRI BsiEI
BsmBI Bsp1407I BspDI BspEI BspLU11I
BspMI BsrBI BsrFI BsrGI BssHII
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstYI BstZ17I Bsu36I CelII
CfoI Cfr10I ClaI CpoI DraI
DraIII DrdI EagI Eam1105I EarI
EclXI Eco47III Eco52I Eco81I EcoO65I
EcoT22I FbaI FseI FspI HaeII
HgaI HhaI Hin1I HinP1I KasI
Ksp632I KspI MfeI MflI MluI
MroI MslI MunI MvnI NaeI
NarI NcoI NgoMIV NheI NotI
NruI NsiI NspV PacI PflFI
PinAI PmaCI PmeI PmlI Psp1406I
PvuI PvuII RsrII SacI SacII
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI StuI
SwaI Tsp45I Tth111I XbaI XcmI
XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 53 enzymes) #
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Acc65I 206 GGTACC G^GTACC
AflII 1432 CTTAAG C^TTAAG
Alw44I 607 GTGCAC G^TGCAC
AlwI 269 GGATC GGATC(4/5)
ApaLI 607 GTGCAC G^TGCAC
AseI 688 ATTAAT AT^TAAT
AsnI 688 ATTAAT AT^TAAT
Asp700I 583 GAAGACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 206 GGTACC G^GTACC
AspHI 607 GTGCAC G[AT]GC[AT]^C
AvaI 299 CTCGAG C^[CT]CG[GA]G
BanI 206 GGTACC G^G[CT][GA]CC
BcgI 921 GCATGTGTATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 409 CCGGG CC^[GC]GG
BfaI 1087 CTAG C^TAG
BfrI 1432 CTTAAG C^TTAAG
BmyI 607 GTGCAC G[AGT]GC[ACT]^C
BsaBI 9 GATTGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiHKAI 607 GTGCAC G[AT]GC[AT]^C
BsiWI 839 CGTACG C^GTACG
BsmI 803 GCATTC GAATGC(1/-1)
BsoBI 299 CTCGAG C^[CT]CG[GA]G
Bsp1286I 607 GTGCAC G[AGT]GC[ACT]^C
BssSI 215 CACGAG CACGAG(-5/-1)
BstXI 1107 CCAGCAAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraII 1576 AGGACCC [GA]G^G[ACGT]CC[CT]
DsaI 493 CCACGG C^C[GA][CT]GG
EcoNI 1621 CCTTGCTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 1576 AGGACCC [GA]G^G[ACGT]CC[CT]
HapII 409 CCGG C^CGG
HindIII 1509 AAGCTT A^AGCTT
HpaI 748 GTTAAC GTT^AAC
HpaII 409 CCGG C^CGG
HphI 909 TCACC GGTGA(8/7)
KpnI 206 GGTACC GGTAC^C
MaeI 1087 CTAG C^TAG
MaeII 946 ACGT A^CGT
MamI 9 GATTGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MspA1I 797 CAGCGG C[AC]G^C[GT]G
MspI 409 CCGG C^CGG
MwoI 1408 GCCTCTCATGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NciI 409 CCGGG CC^[GC]GG
NdeI 1673 CATATG CA^TATG
PaeR7I 299 CTCGAG C^TCGAG
PpuMI 1576 AGGACCC [GA]G^G[AT]CC[CT]
PshAI 1153 GACCTGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 688 ATTAAT AT^TAAT
PstI 479 CTGCAG CTGCA^G
SalI 825 GTCGAC G^TCGAC
SspI 996 AATATT AAT^ATT
TfiI 548 GAATC G^A[AT]TC
XhoI 299 CTCGAG C^TCGAG
XmnI 583 GAAGACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AccI 825 GTCGAC GT^[AC][GT]AC
1222 GTCTAC
BalI 319 TGGCCA TGG^CCA
941 TGGCCA
BbsI 583 GAAGAC GAAGAC(2/6)
988 GAAGAC
BpuAI 583 GAAGAC GAAGAC(2/6)
988 GAAGAC
BsaI 1198 GAGACC GGTCTC(1/5)
296 GGTCTC
BsmFI 1138 GGGAC GGGAC(10/14)
847 GTCCC
BspHI 395 TCATGA T^CATGA
1181 TCATGA
BsrDI 965 GCAATG GCAATG(2/0)
1695 GCAATG
DpnII 270 GATC ^GATC
346 GATC
EaeI 319 TGGCCA [CT]^GGCC[GA]
941 TGGCCA
EcoRV 355 GATATC GAT^ATC
598 GATATC
MaeIII 220 GTTAC ^GT[ACGT]AC
1299 GTTAC
MboI 270 GATC ^GATC
346 GATC
MluNI 319 TGGCCA TGG^CCA
941 TGGCCA
MscI 319 TGGCCA TGG^CCA
941 TGGCCA
NdeII 270 GATC ^GATC
346 GATC
NspI 486 GCATGT [GA]CATG^[CT]
921 GCATGT
PflMI 96 CCAAATTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1669 CCACCATATGG
RcaI 395 TCATGA T^CATGA
1181 TCATGA
Sau3AI 270 GATC ^GATC
346 GATC
SfaNI 1608 GATGC GCATC(5/9)
14 GCATC
SmlI 299 CTCGAG C^T[CT][GA]AG
1432 CTTAAG
Van91I 96 CCAAATTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1669 CCACCATATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 49 enzymes) #
#########################################
AciI AcsI AfaI AluI ApoI
AvaII BbvI BpmI BsaJI BsgI
BsiYI BslI BsmAI BsrI BssKI
BstF5I BstNI Cac8I Cfr13I DdeI
EcoRI EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HincII HindII HinfI
ItaI MboII MnlI MseI MvaI
NlaIII NlaIV PleI RsaI Sau96I
ScrFI SfcI StyI TaqI Tru9I
TseI Tsp509I TspRI TthHB8I