Restriction map for
pFN21AE4517
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# NO CUT ENZYMES ( 114 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AflII AflIII AhdI Alw44I
AocI Aor51HI ApaLI AscI AseI
AsnI Asp718I AspEI AspI AvaI
AviII AvrII BbeI BbrPI BbvCI
BciVI BclI BfrI BglII BlnI
BsaAI BsaBI BsaHI BseAI BsiWI
BsmBI BsoBI Bsp1407I BspDI BspEI
BspLU11I BsrGI BssHII BssSI Bst1107I
BstBI BstZ17I Bsu36I ClaI CpoI
DraI DraIII EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoRI EcoT22I
FbaI FspI Hin1I HincII HindII
HindIII HpaI KasI KpnI MaeII
MamI MfeI MluI MroI MunI
NarI NdeI NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RsrII SalI SapI
ScaI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
SspBI SspI StuI SwaI Tth111I
XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 53 enzymes) #
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AccII 989 CGCG CG^CG
AcsI 1056 GAATTT [GA]^AATT[CT]
AfaI 1640 GTAC GT^AC
AgeI 494 ACCGGT A^CCGGT
ApaI 241 GGGCCC GGGCC^C
ApoI 1056 GAATTT [GA]^AATT[CT]
Asp700I 1360 GAACTCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BamHI 455 GGATCC G^GATCC
BcgI 557 GCACCATCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 1249 CTAG C^TAG
BglI 270 GCCACAGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 516 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 516 GCTGAGC GC^T[ACGT]AGC
BsaI 1540 GAGACC GGTCTC(1/5)
BsaWI 494 ACCGGT [AT]^CCGG[AT]
BsgI 405 GTGCAG GTGCAG(16/14)
BsiEI 991 CGGTCG CG[GA][CT]^CG
BsmAI 1540 GAGAC GTCTC(1/5)
BsmI 1397 GCATTC GAATGC(1/-1)
BspHI 86 TCATGA T^CATGA
BsrBI 1555 CCGCTC CCGCTC(-3/-3)
BsrDI 1503 CATTGC GCAATG(2/0)
BstEII 684 GGTCACC G^GT[ACGT]ACC
BstPI 684 GGTCACC G^GT[ACGT]ACC
BstUI 989 CGCG CG^CG
BstYI 455 GGATCC [GA]^GATC[CT]
CelII 516 GCTGAGC GC^T[ACGT]AGC
EcoNI 1125 CCTCCTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 684 GGTCACC G^GT[ACGT]ACC
EcoRV 813 GATATC GAT^ATC
FseI 1233 GGCCGGCC GGCCGG^CC
HaeII 729 GGCGCT [GA]GCGC^[CT]
HgaI 365 GCGTC GACGC(5/10)
KspI 988 CCGCGG CCGC^GG
MaeI 1249 CTAG C^TAG
MflI 455 GGATCC [GA]^GATC[CT]
MseI 854 TTAA T^TAA
MvnI 989 CGCG CG^CG
NcoI 1229 CCATGG C^CATGG
PinAI 494 ACCGGT A^CCGGT
PleI 768 GACTC GAGTC(4/5)
RcaI 86 TCATGA T^CATGA
RsaI 1640 GTAC GT^AC
SacI 883 GAGCTC GAGCT^C
SacII 988 CCGCGG CCGC^GG
SbfI 54 CCTGCAGG CCTGCA^GG
SexAI 398 ACCTGGT A^CC[AT]GGT
Sse8387I 54 CCTGCAGG CCTGCA^GG
TfiI 832 GAATC G^A[AT]TC
Tru9I 854 TTAA T^TAA
Tsp509I 1057 AATT ^AATT
XhoII 455 GGATCC [GA]^GATC[CT]
XmnI 1360 GAACTCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 13 enzymes) #
###################################
BanI 550 GGCACC G^G[CT][GA]CC
1263 GGTGCC
BbsI 367 GTCTTC GAAGAC(2/6)
1090 GTCTTC
BpuAI 367 GTCTTC GAAGAC(2/6)
1090 GTCTTC
BstAPI 1269 GCAAGATTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1278 GCAGACCCTGC
DrdI 343 GACGGCTGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
358 GACCGCAGCGTC
EarI 43 CTCTTC CTCTTC(1/4)
1138 CTCTTC
HinfI 768 GACTC G^A[ACGT]TC
832 GAATC
Ksp632I 43 CTCTTC CTCTTC(1/4)
1138 CTCTTC
NspI 155 ACATGC [GA]CATG^[CT]
1256 ACATGC
PvuII 309 CAGCTG CAG^CTG
775 CAGCTG
TaqI 10 TCGA T^CGA
1094 TCGA
TthHB8I 10 TCGA T^CGA
1094 TCGA
XcmI 389 CCAAGTGGGACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
689 CCATGACGGTGGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 78 enzymes) #
#########################################
AciI AluI AlwI AlwNI AspHI
AvaII BalI BanII BbvI BcnI
BmyI BpmI BsaJI BseRI BsiHKAI
BsiYI BslI BsmFI Bsp1286I BspMI
BsrFI BsrI BssKI BstF5I BstNI
BstXI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HpaII HphI ItaI MaeIII MboI
MboII MluNI MnlI MscI MslI
MspA1I MspI MvaI MwoI NaeI
NciI NdeII NgoMIV NlaIII NlaIV
PflMI PpuMI PstI Sau3AI Sau96I
ScrFI SfaNI SfcI StyI TseI
Tsp45I TspRI Van91I