Restriction map for
pFN21AE2744
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# NO CUT ENZYMES ( 156 enzymes) #
##################################
Acc65I AccI AccII AccIII AciI
AclI AcsI AflII AflIII AgeI
AhdI Alw44I AocI Aor51HI ApaI
ApaLI ApoI AscI AseI AsnI
Asp718I AspEI AspI AvaI AvrII
BanI BanII BbeI BbrPI BciVI
BclI BfaI BfrI BlnI BlpI
BpmI Bpu1102I BsaBI BsaI BsaWI
BseAI BseRI BsiEI BsiWI BsmBI
BsmFI BsmI BsoBI Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstXI BstZ17I Bsu36I CelII
Cfr10I ClaI CpoI DraI DraII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoO109I EcoO65I EcoRI
EcoRV EcoT22I FauI FbaI FseI
HaeII HgaI HpaI KasI KpnI
KspI MaeI MamI MfeI MluI
MroI MseI MslI MunI MvnI
NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PinAI PleI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacI SacII SalI SapI SbfI
ScaI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI Tru9I
Tsp509I Tth111I XbaI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AatII 298 GACGTC GACGT^C
AcyI 298 GACGTC G[GA]^CG[CT]C
AlwNI 597 CAGTACCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp700I 306 GAAGACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 624 GAGCAC G[AT]GC[AT]^C
AviII 339 TGCGCA TGC^GCA
BamHI 119 GGATCC G^GATCC
BbsI 306 GAAGAC GAAGAC(2/6)
BbvCI 177 GCTGAGG CCTCAGC(-5/-2)
BcgI 244 GCACTCTTCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 91 GCCCGGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 205 AGATCT A^GATCT
BmyI 624 GAGCAC G[AGT]GC[ACT]^C
BpuAI 306 GAAGAC GAAGAC(2/6)
BsaAI 561 CACGTA [CT]AC^GT[GA]
BsaHI 298 GACGTC G[GA]^CG[CT]C
BsgI 475 GTGCAG GTGCAG(16/14)
BsiHKAI 624 GAGCAC G[AT]GC[AT]^C
BsmAI 163 GAGAC GTCTC(1/5)
Bsp1286I 624 GAGCAC G[AGT]GC[ACT]^C
BsrI 116 ACTGG ACTGG(1/-1)
CfoI 340 GCGC GCG^C
DraIII 76 CACCGAGTG CAC[ACGT][ACGT][ACGT]^GTG
EarI 247 CTCTTC CTCTTC(1/4)
FspI 339 TGCGCA TGC^GCA
HhaI 340 GCGC GCG^C
Hin1I 298 GACGTC G[GA]^CG[CT]C
HinP1I 340 GCGC G^CGC
HincII 34 GTCAAC GT[CT]^[GA]AC
HindII 34 GTCAAC GT[CT]^[GA]AC
HindIII 280 AAGCTT A^AGCTT
Ksp632I 247 CTCTTC CTCTTC(1/4)
MspA1I 263 CAGCTG C[AC]G^C[GT]G
PvuII 263 CAGCTG CAG^CTG
SexAI 5 ACCAGGT A^CC[AT]GGT
SfiI 90 GGCCCGGCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
TaqI 253 TCGA T^CGA
Tsp45I 395 GTCAC ^GT[GC]AC
TthHB8I 253 TCGA T^CGA
XcmI 86 CCATGGCCCGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 306 GAAGACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AlwI 120 GATCC GGATC(4/5)
119 GGATC
AvaII 129 GGACC G^G[AT]CC
573 GGACC
BalI 83 TGGCCA TGG^CCA
461 TGGCCA
BbvI 64 GCAGC GCAGC(8/12)
262 GCAGC
BcnI 92 CCCGG CC^[GC]GG
123 CCGGG
BstYI 119 GGATCC [GA]^GATC[CT]
205 AGATCT
EaeI 83 TGGCCA [CT]^GGCC[GA]
461 TGGCCA
EcoNI 327 CCTCTGGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
459 CCTGGCCAAGG
Fnu4HI 64 GCAGC GC^[ACGT]GC
262 GCAGC
HapII 93 CCGG C^CGG
123 CCGG
HinfI 421 GAATC G^A[ACGT]TC
543 GATTC
HpaII 93 CCGG C^CGG
123 CCGG
HphI 75 TCACC GGTGA(8/7)
456 TCACC
ItaI 64 GCAGC GC^[ACGT]GC
262 GCAGC
MaeII 299 ACGT A^CGT
562 ACGT
MaeIII 395 GTCAC ^GT[ACGT]AC
410 GTTAC
MflI 119 GGATCC [GA]^GATC[CT]
205 AGATCT
MluNI 83 TGGCCA TGG^CCA
461 TGGCCA
MscI 83 TGGCCA TGG^CCA
461 TGGCCA
MspI 93 CCGG C^CGG
123 CCGG
NciI 92 CCCGG CC^[GC]GG
123 CCGGG
PflMI 229 CCAGACCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
323 CCAACCTCTGG
PstI 103 CTGCAG CTGCA^G
260 CTGCAG
SfcI 103 CTGCAG C^T[GA][CT]AG
260 CTGCAG
TfiI 421 GAATC G^A[AT]TC
543 GATTC
TseI 64 GCAGC G^C[AT]GC
262 GCAGC
Van91I 229 CCAGACCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
323 CCAACCTCTGG
XhoII 119 GGATCC [GA]^GATC[CT]
205 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 33 enzymes) #
#########################################
AfaI AluI BsaJI BsiYI BslI
BssKI BstF5I BstNI Cac8I Cfr13I
DdeI DpnII DsaI EcoRII EcoT14I
FokI HaeIII MboI MboII MnlI
MvaI MwoI NcoI NdeII NlaIII
NlaIV RsaI Sau3AI Sau96I ScrFI
SfaNI StyI TspRI