Restriction map for
pFN21AE4459
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# NO CUT ENZYMES ( 108 enzymes) #
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AatII Acc65I AccI AccIII AclI
AflII AflIII AgeI AhdI AocI
Aor51HI ApaI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AviII
AvrII BamHI BbrPI BciVI BclI
BfaI BfrI BglI BlnI BsaBI
BseAI BsgI BsiEI BsiWI BsmFI
Bsp1407I BspDI BspEI BspLU11I BspMI
BsrBI BsrGI Bst1107I BstBI BstZ17I
Bsu36I ClaI CpoI DraII DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO109I EcoRI
EcoRV FbaI FseI FspI HpaI
KpnI MaeI MamI MluI MroI
NheI NotI NruI NspI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PpuMI PshBI Psp1406I
PvuI PvuII RsrII SalI SapI
SbfI ScaI SexAI SfiI SfuI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI TfiI Tsp45I Tth111I
XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 50 enzymes) #
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Alw44I 207 GTGCAC G^TGCAC
AlwNI 999 CAGCAACTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 207 GTGCAC G^TGCAC
AvaII 616 GGTCC G^G[AT]CC
BalI 758 TGGCCA TGG^CCA
BbsI 1062 GAAGAC GAAGAC(2/6)
BbvCI 1268 CCTCAGC CCTCAGC(-5/-2)
BcgI 143 GCATCTCCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 555 AGATCT A^GATCT
BlpI 1026 GCTTAGC GC^T[ACGT]AGC
Bpu1102I 1026 GCTTAGC GC^T[ACGT]AGC
BpuAI 1062 GAAGAC GAAGAC(2/6)
BsaAI 595 TACGTG [CT]AC^GT[GA]
BsaWI 563 ACCGGA [AT]^CCGG[AT]
BspHI 798 TCATGA T^CATGA
BsrDI 539 GCAATG GCAATG(2/0)
BsrFI 119 GCCGGC [GA]^CCGG[CT]
BssHII 20 GCGCGC G^CGCGC
BssSI 187 CTCGTG CACGAG(-5/-1)
BstAPI 43 GCACTGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1153 GGTAACC G^GT[ACGT]ACC
BstPI 1153 GGTAACC G^GT[ACGT]ACC
BstYI 555 AGATCT [GA]^GATC[CT]
CelII 1026 GCTTAGC GC^T[ACGT]AGC
Cfr10I 119 GCCGGC [GA]^CCGG[CT]
EcoO65I 1153 GGTAACC G^GT[ACGT]ACC
EcoT22I 337 ATGCAT ATGCA^T
HincII 427 GTCAAC GT[CT]^[GA]AC
HindII 427 GTCAAC GT[CT]^[GA]AC
KspI 98 CCGCGG CCGC^GG
MaeII 596 ACGT A^CGT
MflI 555 AGATCT [GA]^GATC[CT]
MluNI 758 TGGCCA TGG^CCA
MscI 758 TGGCCA TGG^CCA
MspA1I 98 CCGCGG C[AC]G^C[GT]G
NaeI 119 GCCGGC GCC^GGC
NcoI 741 CCATGG C^CATGG
NdeI 770 CATATG CA^TATG
NgoMIV 119 GCCGGC G^CCGGC
NsiI 337 ATGCAT ATGCA^T
PflMI 834 CCAAAGCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 420 GACAATTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 798 TCATGA T^CATGA
SacI 259 GAGCTC GAGCT^C
SacII 98 CCGCGG CCGC^GG
SgrAI 118 CGCCGGCG C[GA]^CCGG[CT]G
SwaI 318 ATTTAAAT ATTT^AAAT
Van91I 834 CCAAAGCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1172 CCACCATCTCCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 555 AGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 25 enzymes) #
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AcsI 316 GAATTT [GA]^AATT[CT]
1422 AAATTT
AlwI 435 GATCC GGATC(4/5)
657 GGATC
ApoI 316 GAATTT [GA]^AATT[CT]
1422 AAATTT
BanII 85 GAGCCC G[GA]GC[CT]^C
259 GAGCTC
BsmBI 497 CGTCTC CGTCTC(1/5)
778 GAGACG
BsmI 1167 GAATGC GAATGC(1/-1)
449 GCATTC
BstXI 297 CCATGAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1175 CCATCTCCGTGG
DraI 319 TTTAAA TTT^AAA
1419 TTTAAA
EcoT14I 686 CCAAGG C^C[AT][AT]GG
741 CCATGG
HindIII 670 AAGCTT A^AGCTT
837 AAGCTT
HinfI 1056 GACTC G^A[ACGT]TC
1348 GAGTC
MaeIII 1154 GTAAC ^GT[ACGT]AC
1204 GTTAC
MfeI 422 CAATTG C^AATTG
1160 CAATTG
MslI 340 CATGGGAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1176 CATCTCCGTG
MunI 422 CAATTG C^AATTG
1160 CAATTG
PleI 1056 GACTC GAGTC(4/5)
1348 GAGTC
PstI 127 CTGCAG CTGCA^G
202 CTGCAG
SfaNI 975 GATGC GCATC(5/9)
143 GCATC
SfcI 127 CTGCAG C^T[GA][CT]AG
202 CTGCAG
SmaI 88 CCCGGG CCC^GGG
845 CCCGGG
SmlI 514 CTCAAG C^T[CT][GA]AG
1105 CTTGAG
StyI 686 CCAAGG C^C[AT][AT]GG
741 CCATGG
TspRI 44 CACTG CA[GC]TG[ACGT][ACGT]^
205 CAGTG
XmaCI 88 CCCGGG C^CCGGG
845 CCCGGG
XmaI 88 CCCGGG C^CCGGG
845 CCCGGG
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# 3 OR MORE CUT ENZYMES ( 75 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AspHI AvaI BanI BbeI BbvI
BcnI BmyI BpmI BsaHI BsaI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmAI BsoBI Bsp1286I BsrI BssKI
BstF5I BstNI BstUI Cac8I CfoI
Cfr13I DdeI DpnII DsaI EaeI
EarI EcoRII FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HpaII HphI ItaI
KasI Ksp632I MboI MboII MnlI
MseI MspI MvaI MvnI MwoI
NarI NciI NdeII NlaIII NlaIV
RsaI Sau3AI Sau96I ScrFI SfoI
TaqI Tru9I TseI Tsp509I TthHB8I