Restriction map for
pFN21AB9420
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# NO CUT ENZYMES ( 119 enzymes) #
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AatII AccII AccIII AclI AcyI
AflII AgeI AhdI AlwNI AocI
Aor51HI ApaI AscI AspEI AspI
AviII AvrII BamHI BanII BbeI
BbrPI BbvCI BcgI BciVI BclI
BfrI BglI BglII BlnI BlpI
BpmI Bpu1102I BsaHI BsaWI BseAI
BsiEI BsiWI Bsp1407I BspDI BspEI
BspMI BsrBI BsrGI BssHII BssSI
BstAPI BstEII BstPI BstUI Bsu36I
CelII CfoI ClaI CpoI DrdI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoRI FauI
FbaI FseI FspI HaeII HgaI
HhaI Hin1I HinP1I HincII HindII
HpaI KasI KspI MfeI MluI
MroI MunI MvnI NaeI NarI
NcoI NgoMIV NotI NruI PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PshAI Psp1406I PvuI
RsrII SacI SacII SalI SapI
SbfI SexAI SfiI SfoI SgrAI
SmlI SnaBI SpeI Sse8387I SspBI
Tth111I Van91I XbaI XhoI
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# ONE CUT ENZYMES ( 62 enzymes) #
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Acc65I 1058 GGTACC G^GTACC
AccI 392 GTATAC GT^[AC][GT]AC
Alw44I 689 GTGCAC G^TGCAC
ApaLI 689 GTGCAC G^TGCAC
AseI 961 ATTAAT AT^TAAT
AsnI 961 ATTAAT AT^TAAT
Asp700I 1511 GAAATTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1058 GGTACC G^GTACC
AspHI 689 GTGCAC G[AT]GC[AT]^C
BalI 1237 TGGCCA TGG^CCA
BanI 1058 GGTACC G^G[CT][GA]CC
BbvI 104 GCAGC GCAGC(8/12)
BmyI 689 GTGCAC G[AGT]GC[ACT]^C
BsaAI 893 TACGTG [CT]AC^GT[GA]
BsaBI 463 GATGGATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiHKAI 689 GTGCAC G[AT]GC[AT]^C
BsmBI 149 CGTCTC CGTCTC(1/5)
BsmI 162 GCATTC GAATGC(1/-1)
Bsp1286I 689 GTGCAC G[AGT]GC[ACT]^C
BsrFI 1061 ACCGGC [GA]^CCGG[CT]
Bst1107I 392 GTATAC GTA^TAC
BstF5I 834 GGATG GGATG(2/0)
BstXI 330 CCAAGCTTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 392 GTATAC GTA^TAC
Cfr10I 1061 ACCGGC [GA]^CCGG[CT]
DraII 1496 AGGACCC [GA]G^G[ACGT]CC[CT]
DraIII 218 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 1178 CCGTGG C^C[GA][CT]GG
EarI 1393 GAAGAG CTCTTC(1/4)
EcoO109I 1496 AGGACCC [GA]G^G[ACGT]CC[CT]
EcoRV 467 GATATC GAT^ATC
EcoT14I 240 CCTTGG C^C[AT][AT]GG
EcoT22I 160 ATGCAT ATGCA^T
Fnu4HI 104 GCAGC GC^[ACGT]GC
FokI 834 GGATG GGATG(9/13)
ItaI 104 GCAGC GC^[ACGT]GC
KpnI 1058 GGTACC GGTAC^C
Ksp632I 1393 GAAGAG CTCTTC(1/4)
MamI 463 GATGGATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 1237 TGGCCA TGG^CCA
MscI 1237 TGGCCA TGG^CCA
MspA1I 1388 CAGCTG C[AC]G^C[GT]G
NdeI 605 CATATG CA^TATG
NheI 984 GCTAGC G^CTAGC
NsiI 160 ATGCAT ATGCA^T
PpuMI 1496 AGGACCC [GA]G^G[AT]CC[CT]
PshBI 961 ATTAAT AT^TAAT
PstI 443 CTGCAG CTGCA^G
PvuII 1388 CAGCTG CAG^CTG
ScaI 1094 AGTACT AGT^ACT
SfaNI 930 GCATC GCATC(5/9)
SmaI 1500 CCCGGG CCC^GGG
SphI 1559 GCATGC GCATG^C
SspI 1464 AATATT AAT^ATT
StuI 139 AGGCCT AGG^CCT
StyI 240 CCTTGG C^C[AT][AT]GG
SwaI 419 ATTTAAAT ATTT^AAAT
TseI 104 GCAGC G^C[AT]GC
XcmI 885 CCAAATTCTACGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 1500 CCCGGG C^CCGGG
XmaI 1500 CCCGGG C^CCGGG
XmnI 1511 GAAATTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 35 enzymes) #
###################################
AciI 210 GCGG CCGC(-3/-1)
429 GCGG
AflIII 1112 ACATGT A^C[GA][CT]GT
1407 ACATGT
AvaI 1004 CCCGAG C^[CT]CG[GA]G
1500 CCCGGG
AvaII 1429 GGACC G^G[AT]CC
1497 GGACC
BbsI 1154 GAAGAC GAAGAC(2/6)
1550 GAAGAC
BcnI 1500 CCCGG CC^[GC]GG
1501 CCGGG
BpuAI 1154 GAAGAC GAAGAC(2/6)
1550 GAAGAC
BsaI 213 GAGACC GGTCTC(1/5)
524 GGTCTC
BseRI 955 CTCCTC GAGGAG(10/8)
1258 GAGGAG
BsgI 696 CTGCAC GTGCAG(16/14)
1192 CTGCAC
BsmFI 340 GGGAC GGGAC(10/14)
1428 GGGAC
BsoBI 1004 CCCGAG C^[CT]CG[GA]G
1500 CCCGGG
BspHI 291 TCATGA T^CATGA
851 TCATGA
BspLU11I 1112 ACATGT A^CATGT
1407 ACATGT
BsrDI 206 CATTGC GCAATG(2/0)
256 GCAATG
BsrI 177 CCAGT ACTGG(1/-1)
360 CCAGT
BstBI 1418 TTCGAA TT^CGAA
1508 TTCGAA
Cfr13I 1429 GGACC G^G[ACGT]CC
1497 GGACC
DraI 420 TTTAAA TTT^AAA
732 TTTAAA
EaeI 1063 CGGCCA [CT]^GGCC[GA]
1237 TGGCCA
HapII 1062 CCGG C^CGG
1501 CCGG
HindIII 332 AAGCTT A^AGCTT
589 AAGCTT
HpaII 1062 CCGG C^CGG
1501 CCGG
HphI 319 GGTGA GGTGA(8/7)
364 TCACC
MslI 252 CATGGCAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
852 CATGAGTATG
MspI 1062 CCGG C^CGG
1501 CCGG
MwoI 201 GCCCCCATTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
1390 GCTGAAGAGGC
NciI 1500 CCCGG CC^[GC]GG
1501 CCGGG
NspV 1418 TTCGAA TT^CGAA
1508 TTCGAA
PleI 1327 GACTC GAGTC(4/5)
516 GAGTC
RcaI 291 TCATGA T^CATGA
851 TCATGA
Sau96I 1429 GGACC G^G[ACGT]CC
1497 GGACC
SfcI 368 CTACAG C^T[GA][CT]AG
443 CTGCAG
SfuI 1418 TTCGAA TT^CGAA
1508 TTCGAA
TspRI 222 CAGTG CA[GC]TG[ACGT][ACGT]^
437 CACTG
#########################################
# 3 OR MORE CUT ENZYMES ( 42 enzymes) #
#########################################
AcsI AfaI AluI AlwI ApoI
BfaI BsaJI BsiYI BslI BsmAI
BssKI BstNI BstYI Cac8I DdeI
DpnII EcoRII HaeIII HinfI MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MvaI NdeII NlaIII
NlaIV NspI RsaI Sau3AI ScrFI
TaqI TfiI Tru9I Tsp45I Tsp509I
TthHB8I XhoII