Restriction map for
pFN21AE1296
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# NO CUT ENZYMES ( 116 enzymes) #
##################################
AatII AccI AccII AclI AcyI
AflII AgeI AhdI Aor51HI AscI
AseI AsnI AspEI AspI AviII
AvrII BbeI BbrPI BcgI BciVI
BfrI BglI BglII BlnI BlpI
Bpu1102I BsaAI BsaBI BsaHI BsaI
BsiEI BsiWI BsmBI Bsp1407I BspDI
BspHI BsrBI BsrGI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstUI
BstXI BstZ17I CelII ClaI CpoI
DraI DrdI EagI Eam1105I EclXI
Eco47III Eco52I EcoO65I EcoRV EcoT22I
FseI FspI Hin1I HindIII HpaI
KasI KspI MamI MfeI MluI
MunI MvnI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacI SacII SalI SapI SbfI
SexAI SfiI SfoI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SspBI
SspI SwaI Tth111I Van91I XbaI
XhoI
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# ONE CUT ENZYMES ( 39 enzymes) #
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Acc65I 717 GGTACC G^GTACC
AflIII 745 ACATGT A^C[GA][CT]GT
Alw44I 688 GTGCAC G^TGCAC
ApaI 1160 GGGCCC GGGCC^C
ApaLI 688 GTGCAC G^TGCAC
Asp700I 125 GAAAGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 717 GGTACC G^GTACC
BalI 290 TGGCCA TGG^CCA
BamHI 854 GGATCC G^GATCC
BbsI 1047 GAAGAC GAAGAC(2/6)
BbvCI 280 CCTCAGC CCTCAGC(-5/-2)
BpuAI 1047 GAAGAC GAAGAC(2/6)
BsmI 485 GAATGC GAATGC(1/-1)
BspLU11I 745 ACATGT A^CATGT
BsrFI 926 GCCGGT [GA]^CCGG[CT]
CfoI 934 GCGC GCG^C
Cfr10I 926 GCCGGT [GA]^CCGG[CT]
DraIII 1231 CACCGGGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 509 CCATGG C^C[GA][CT]GG
EaeI 290 TGGCCA [CT]^GGCC[GA]
EcoNI 1482 CCTGCGGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 75 GAATTC G^AATTC
FauI 1437 GCGGG CCCGC(4/6)
HaeII 933 GGCGCT [GA]GCGC^[CT]
HgaI 396 GACGC GACGC(5/10)
HhaI 934 GCGC GCG^C
HinP1I 934 GCGC G^CGC
KpnI 717 GGTACC GGTAC^C
MaeII 836 ACGT A^CGT
MluNI 290 TGGCCA TGG^CCA
MscI 290 TGGCCA TGG^CCA
MseI 473 TTAA T^TAA
NcoI 509 CCATGG C^CATGG
NspI 745 ACATGT [GA]CATG^[CT]
SmaI 267 CCCGGG CCC^GGG
Tru9I 473 TTAA T^TAA
XmaCI 267 CCCGGG C^CCGGG
XmaI 267 CCCGGG C^CCGGG
XmnI 125 GAAAGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AccIII 195 TCCGGA T^CCGGA
392 TCCGGA
AocI 859 CCTGAGG CC^T[ACGT]AGG
1327 CCTGAGG
BanI 255 GGTGCC G^G[CT][GA]CC
717 GGTACC
BclI 155 TGATCA T^GATCA
1397 TGATCA
BsaWI 195 TCCGGA [AT]^CCGG[AT]
392 TCCGGA
BseAI 195 TCCGGA T^CCGGA
392 TCCGGA
BseRI 1123 GAGGAG GAGGAG(10/8)
1222 GAGGAG
BsgI 1145 CTGCAC GTGCAG(16/14)
942 GTGCAG
BsmFI 271 GGGAC GGGAC(10/14)
69 GTCCC
BspEI 195 TCCGGA T^CCGGA
392 TCCGGA
BspMI 1295 ACCTGC ACCTGC(4/8)
1481 ACCTGC
BsrDI 650 GCAATG GCAATG(2/0)
953 GCAATG
BstAPI 944 GCAGCCTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1251 GCAGAAGCTGC
BstYI 219 AGATCC [GA]^GATC[CT]
854 GGATCC
Bsu36I 859 CCTGAGG CC^T[ACGT]AGG
1327 CCTGAGG
Eco81I 859 CCTGAGG CC^T[ACGT]AGG
1327 CCTGAGG
EcoT14I 509 CCATGG C^C[AT][AT]GG
902 CCAAGG
FbaI 155 TGATCA T^GATCA
1397 TGATCA
MflI 219 AGATCC [GA]^GATC[CT]
854 GGATCC
MroI 195 TCCGGA T^CCGGA
392 TCCGGA
PleI 178 GACTC GAGTC(4/5)
1128 GAGTC
PpuMI 518 GGGTCCT [GA]G^G[AT]CC[CT]
1490 AGGACCT
PstI 1258 CTGCAG CTGCA^G
1465 CTGCAG
ScaI 203 AGTACT AGT^ACT
1355 AGTACT
SfaNI 439 GATGC GCATC(5/9)
542 GATGC
SfcI 1258 CTGCAG C^T[GA][CT]AG
1465 CTGCAG
SmlI 712 CTCAAG C^T[CT][GA]AG
1207 CTTGAG
StuI 582 AGGCCT AGG^CCT
695 AGGCCT
StyI 509 CCATGG C^C[AT][AT]GG
902 CCAAGG
TaqI 123 TCGA T^CGA
593 TCGA
TthHB8I 123 TCGA T^CGA
593 TCGA
XhoII 219 AGATCC [GA]^GATC[CT]
854 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 71 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI AspHI AvaI AvaII
BanII BbvI BcnI BfaI BmyI
BpmI BsaJI BsiHKAI BsiYI BslI
BsmAI BsoBI Bsp1286I BsrI BssKI
BstF5I BstNI Cac8I Cfr13I DdeI
DpnII DraII EarI EcoO109I EcoRII
Fnu4HI FokI HaeIII HapII HincII
HindII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeIII MboI MboII
MnlI MslI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
PvuII RsaI Sau3AI Sau96I ScrFI
TfiI TseI Tsp45I Tsp509I TspRI
XcmI