Restriction map for
pFN21AE6211
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# NO CUT ENZYMES ( 167 enzymes) #
##################################
Acc65I AccI AccIII AclI AcsI
AflII AflIII AgeI AhdI Alw44I
AlwI AlwNI AocI ApaI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspHI AspI AvaI
AvrII BamHI BanI BbeI BbrPI
BbsI BcgI BciVI BclI BcnI
BfaI BfrI BglII BlnI BpuAI
BsaBI BsaI BsaWI BseAI BsgI
BsiEI BsiHKAI BsiWI BsmBI BsmFI
BsmI BsoBI Bsp1407I BspDI BspEI
BspLU11I BspMI BsrBI BsrDI BsrGI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstXI BstYI BstZ17I Bsu36I
ClaI CpoI DpnII DraI DraII
DrdI DsaI EagI Eam1105I EclXI
Eco52I Eco81I EcoNI EcoO109I EcoO65I
EcoRI EcoRV EcoT14I EcoT22I FbaI
FseI HindIII HinfI HpaI KasI
KpnI KspI MaeI MaeIII MamI
MboI MfeI MflI MluI MroI
MslI MunI NarI NciI NcoI
NdeI NdeII NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PinAI PleI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI PvuII RsrII SacI SacII
SalI SapI Sau3AI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI StyI SwaI
TaqI TfiI Tsp45I Tsp509I Tth111I
TthHB8I XbaI XhoI XhoII XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 38 enzymes) #
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AatII 430 GACGTC GACGT^C
Aor51HI 234 AGCGCT AGC^GCT
AviII 539 TGCGCA TGC^GCA
BalI 93 TGGCCA TGG^CCA
BanII 144 GAGCCC G[GA]GC[CT]^C
BbvCI 589 GCTGAGG CCTCAGC(-5/-2)
BpmI 547 CTCCAG CTGGAG(16/14)
BsaJI 455 CCCTGG C^C[ACGT][ACGT]GG
BsiYI 159 CCAGTACGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 159 CCAGTACGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI 181 GAGAC GTCTC(1/5)
BspHI 74 TCATGA T^CATGA
BsrFI 598 GCCGGC [GA]^CCGG[CT]
Cfr10I 598 GCCGGC [GA]^CCGG[CT]
DraIII 45 CACCGAGTG CAC[ACGT][ACGT][ACGT]^GTG
EarI 460 GAAGAG CTCTTC(1/4)
Eco47III 234 AGCGCT AGC^GCT
FauI 530 CCCGC CCCGC(4/6)
FspI 539 TGCGCA TGC^GCA
HaeII 234 AGCGCT [GA]GCGC^[CT]
HapII 599 CCGG C^CGG
HgaI 476 GCGTC GACGC(5/10)
HincII 433 GTCAAC GT[CT]^[GA]AC
HindII 433 GTCAAC GT[CT]^[GA]AC
HpaII 599 CCGG C^CGG
Ksp632I 460 GAAGAG CTCTTC(1/4)
MluNI 93 TGGCCA TGG^CCA
MscI 93 TGGCCA TGG^CCA
MseI 365 TTAA T^TAA
MspI 599 CCGG C^CGG
NaeI 598 GCCGGC GCC^GGC
NgoMIV 598 GCCGGC G^CCGGC
NlaIV 545 GGCTCC GG[ACGT]^[ACGT]CC
PflMI 159 CCAGTACGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RcaI 74 TCATGA T^CATGA
Tru9I 365 TTAA T^TAA
Van91I 159 CCAGTACGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 155 CCAGCCAGTACGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AccII 325 CGCG CG^CG
358 CGCG
AcyI 430 GACGTC G[GA]^CG[CT]C
475 GGCGTC
BglI 86 GCCCCTCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
586 GCCGCTGAGGC
BlpI 36 GCTGAGC GC^T[ACGT]AGC
237 GCTCAGC
BmyI 84 GTGCCC G[AGT]GC[ACT]^C
144 GAGCCC
Bpu1102I 36 GCTGAGC GC^T[ACGT]AGC
237 GCTCAGC
BsaAI 163 TACGTG [CT]AC^GT[GA]
226 TACGTG
BsaHI 430 GACGTC G[GA]^CG[CT]C
475 GGCGTC
Bsp1286I 84 GTGCCC G[AGT]GC[ACT]^C
144 GAGCCC
BstAPI 44 GCACCGAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
308 GCAACGGCTGC
BstF5I 345 CATCC GGATG(2/0)
441 GGATG
BstUI 325 CGCG CG^CG
358 CGCG
CelII 36 GCTGAGC GC^T[ACGT]AGC
237 GCTCAGC
EaeI 93 TGGCCA [CT]^GGCC[GA]
152 CGGCCA
FokI 345 CATCC GGATG(9/13)
441 GGATG
Hin1I 430 GACGTC G[GA]^CG[CT]C
475 GGCGTC
MboII 460 GAAGA GAAGA(8/7)
125 TCTTC
MspA1I 276 CCGCTG C[AC]G^C[GT]G
587 CCGCTG
MvnI 325 CGCG CG^CG
358 CGCG
PstI 372 CTGCAG CTGCA^G
392 CTGCAG
SfaNI 492 GATGC GCATC(5/9)
533 GCATC
SfcI 372 CTGCAG C^T[GA][CT]AG
392 CTGCAG
SmlI 133 CTCAAG C^T[CT][GA]AG
250 CTCAAG
#########################################
# 3 OR MORE CUT ENZYMES ( 30 enzymes) #
#########################################
AciI AfaI AluI AvaII BbvI
BseRI BsrI BssKI BstNI Cac8I
CfoI Cfr13I DdeI EcoRII Fnu4HI
HaeIII HhaI HinP1I HphI ItaI
MaeII MnlI MvaI MwoI NlaIII
RsaI Sau96I ScrFI TseI TspRI