Restriction map for
pFN21AE9666
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# NO CUT ENZYMES ( 108 enzymes) #
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AatII Acc65I AclI AcyI AflII
AgeI Alw44I ApaI ApaLI AscI
Asp718I AspI AvaI AviII AvrII
BalI BanI BbeI BbvCI BcgI
BciVI BfrI BlnI BlpI Bpu1102I
BsaBI BsaHI BsiEI BsiWI BsmBI
BsoBI BspDI BsrBI BssHII Bst1107I
BstEII BstPI BstXI BstZ17I CelII
ClaI CpoI DraI DraII DraIII
DrdI EaeI EagI EclXI Eco52I
EcoNI EcoO109I EcoO65I EcoT22I FauI
FseI FspI Hin1I HincII HindII
HpaI KasI KpnI KspI MamI
MluI MluNI MscI NaeI NarI
NgoMIV NheI NotI NruI NsiI
PacI PaeR7I PflFI PflMI PinAI
PmeI PpuMI PshAI Psp1406I PvuI
RsrII SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SwaI Tth111I Van91I XbaI XcmI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AccI 1132 GTAGAC GT^[AC][GT]AC
AccII 2453 CGCG CG^CG
AccIII 2410 TCCGGA T^CCGGA
AlwNI 3107 CAGTTTCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 2100 CCTGAGG CC^T[ACGT]AGG
Aor51HI 60 AGCGCT AGC^GCT
AseI 2191 ATTAAT AT^TAAT
AsnI 2191 ATTAAT AT^TAAT
BamHI 2836 GGATCC G^GATCC
BbrPI 1438 CACGTG CAC^GTG
BclI 2400 TGATCA T^GATCA
BglI 2330 GCCTGTTCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 3496 AGATCT A^GATCT
BseAI 2410 TCCGGA T^CCGGA
BsmI 2339 GCATTC GAATGC(1/-1)
BspEI 2410 TCCGGA T^CCGGA
BspHI 2727 TCATGA T^CATGA
BsrFI 1569 ACCGGC [GA]^CCGG[CT]
BssSI 3484 CTCGTG CACGAG(-5/-1)
BstUI 2453 CGCG CG^CG
Bsu36I 2100 CCTGAGG CC^T[ACGT]AGG
Cfr10I 1569 ACCGGC [GA]^CCGG[CT]
Eco47III 60 AGCGCT AGC^GCT
Eco81I 2100 CCTGAGG CC^T[ACGT]AGG
EcoRV 2434 GATATC GAT^ATC
FbaI 2400 TGATCA T^GATCA
HaeII 60 AGCGCT [GA]GCGC^[CT]
HindIII 322 AAGCTT A^AGCTT
MfeI 2527 CAATTG C^AATTG
MroI 2410 TCCGGA T^CCGGA
MunI 2527 CAATTG C^AATTG
MvnI 2453 CGCG CG^CG
NdeI 1652 CATATG CA^TATG
PmaCI 1438 CACGTG CAC^GTG
PmlI 1438 CACGTG CAC^GTG
PshBI 2191 ATTAAT AT^TAAT
RcaI 2727 TCATGA T^CATGA
SacI 1178 GAGCTC GAGCT^C
SspI 2498 AATATT AAT^ATT
StuI 2801 AGGCCT AGG^CCT
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# TWO CUT ENZYMES ( 19 enzymes) #
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AflIII 744 ACATGT A^C[GA][CT]GT
3058 ACATGT
AhdI 286 GACTTAGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
958 GACGTGAAGTC
AspEI 286 GACTTAGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
958 GACGTGAAGTC
BsaAI 1369 CACGTA [CT]AC^GT[GA]
1438 CACGTG
BsaI 3268 GAGACC GGTCTC(1/5)
843 GGTCTC
BsgI 1225 GTGCAG GTGCAG(16/14)
1514 GTGCAG
Bsp1407I 1585 TGTACA T^GTACA
3336 TGTACA
BspLU11I 744 ACATGT A^CATGT
3058 ACATGT
BsrDI 2759 CATTGC GCAATG(2/0)
661 GCAATG
BsrGI 1585 TGTACA T^GTACA
3336 TGTACA
BstAPI 1120 GCACCTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1330 GCAACACTTGC
BstBI 203 TTCGAA TT^CGAA
410 TTCGAA
Eam1105I 286 GACTTAGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
958 GACGTGAAGTC
NspV 203 TTCGAA TT^CGAA
410 TTCGAA
PstI 1358 CTGCAG CTGCA^G
1877 CTGCAG
PvuII 2789 CAGCTG CAG^CTG
3350 CAGCTG
SfuI 203 TTCGAA TT^CGAA
410 TTCGAA
SmlI 548 CTCAAG C^T[CT][GA]AG
1627 CTTGAG
SspBI 1585 TGTACA T^GTACA
3336 TGTACA
#########################################
# 3 OR MORE CUT ENZYMES ( 91 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI Asp700I AspHI AvaII BanII
BbsI BbvI BcnI BfaI BmyI
BpmI BpuAI BsaJI BsaWI BseRI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BspMI BsrI BssKI BstF5I
BstNI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DsaI EarI EcoRI
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HgaI HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MwoI NciI NcoI NdeII
NlaIII NlaIV NspI PleI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII
XmnI