Restriction map for
pFN21AE3914
##################################
# NO CUT ENZYMES ( 163 enzymes) #
##################################
Acc65I AccII AccIII AclI AcsI
AflII AflIII AgeI AhdI Alw44I
AlwNI AocI Aor51HI ApaI ApaLI
ApoI AscI Asp700I Asp718I AspEI
AspI AviII AvrII BalI BamHI
BanI BanII BbeI BbrPI BbsI
BbvCI BciVI BclI BfaI BfrI
BglI BglII BlnI BlpI Bpu1102I
BpuAI BsaAI BsaBI BseAI BsiEI
BsiWI BsmI Bsp1407I BspDI BspEI
BspLU11I BspMI BsrDI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstF5I BstNI BstPI BstUI
BstXI BstZ17I Bsu36I CelII Cfr10I
ClaI DraI DraII DraIII DrdI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoRI
EcoRII EcoRV EcoT14I EcoT22I FauI
FbaI FokI FseI FspI HindIII
HpaI KasI KpnI KspI MaeI
MamI MfeI MluI MluNI MroI
MscI MspA1I MunI MvaI MvnI
NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI Psp1406I PstI PvuI PvuII
SacI SacII SapI SbfI ScaI
SexAI SfcI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI StyI
SwaI Tth111I Van91I XbaI XhoI
XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
AatII 461 GACGTC GACGT^C
AccI 458 GTCGAC GT^[AC][GT]AC
AcyI 461 GACGTC G[GA]^CG[CT]C
AseI 327 ATTAAT AT^TAAT
AsnI 327 ATTAAT AT^TAAT
AspHI 675 GTGCTC G[AT]GC[AT]^C
AvaI 559 CCCGAG C^[CT]CG[GA]G
BcgI 460 CGACGTCGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 600 CCGGG CC^[GC]GG
BmyI 675 GTGCTC G[AGT]GC[ACT]^C
BpmI 490 CTGGAG CTGGAG(16/14)
BsaHI 461 GACGTC G[GA]^CG[CT]C
BsaI 187 GAGACC GGTCTC(1/5)
BsaWI 635 ACCGGA [AT]^CCGG[AT]
BseRI 289 GAGGAG GAGGAG(10/8)
BsiHKAI 675 GTGCTC G[AT]GC[AT]^C
BsmBI 5 GAGACG CGTCTC(1/5)
BsoBI 559 CCCGAG C^[CT]CG[GA]G
Bsp1286I 675 GTGCTC G[AGT]GC[ACT]^C
BspHI 614 TCATGA T^CATGA
BsrBI 143 CCGCTC CCGCTC(-3/-3)
BssKI 600 CCGGG ^CC[ACGT]GG
BstYI 346 AGATCC [GA]^GATC[CT]
CpoI 424 CGGTCCG CG^G[AT]CCG
DsaI 594 CCGTGG C^C[GA][CT]GG
HgaI 7 GACGC GACGC(5/10)
Hin1I 461 GACGTC G[GA]^CG[CT]C
HincII 458 GTCGAC GT[CT]^[GA]AC
HindII 458 GTCGAC GT[CT]^[GA]AC
MaeII 462 ACGT A^CGT
MflI 346 AGATCC [GA]^GATC[CT]
MseI 328 TTAA T^TAA
MslI 615 CATGAAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NciI 600 CCGGG CC^[GC]GG
NlaIV 70 GGGTCC GG[ACGT]^[ACGT]CC
PleI 456 GAGTC GAGTC(4/5)
PshBI 327 ATTAAT AT^TAAT
RcaI 614 TCATGA T^CATGA
RsrII 424 CGGTCCG CG^G[AT]CCG
SalI 458 GTCGAC G^TCGAC
ScrFI 600 CCGGG CC^[ACGT]GG
SfaNI 662 GCATC GCATC(5/9)
SmlI 409 CTTGAG C^T[CT][GA]AG
Tru9I 328 TTAA T^TAA
Tsp45I 197 GTGAC ^GT[GC]AC
Tsp509I 509 AATT ^AATT
XhoII 346 AGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AfaI 152 GTAC GT^AC
356 GTAC
AlwI 347 GATCC GGATC(4/5)
514 GGATC
BsaJI 428 CCGAGG C^C[ACGT][ACGT]GG
594 CCGTGG
BsgI 573 CTGCAC GTGCAG(16/14)
499 GTGCAG
BsiYI 594 CCGTGGCCGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
636 CCGGAAAGTGG
BslI 594 CCGTGGCCGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
636 CCGGAAAGTGG
BsmFI 53 GTCCC GGGAC(10/14)
236 GTCCC
DdeI 232 CTTAG C^T[ACGT]AG
371 CTGAG
EaeI 397 CGGCCA [CT]^GGCC[GA]
597 TGGCCG
EarI 184 GAAGAG CTCTTC(1/4)
453 GAAGAG
HaeII 30 AGCGCC [GA]GCGC^[CT]
647 GGCGCT
HapII 600 CCGG C^CGG
636 CCGG
HpaII 600 CCGG C^CGG
636 CCGG
Ksp632I 184 GAAGAG CTCTTC(1/4)
453 GAAGAG
MboII 184 GAAGA GAAGA(8/7)
453 GAAGA
MspI 600 CCGG C^CGG
636 CCGG
RsaI 152 GTAC GT^AC
356 GTAC
TspRI 195 CAGTG CA[GC]TG[ACGT][ACGT]^
475 CAGTG
#########################################
# 3 OR MORE CUT ENZYMES ( 30 enzymes) #
#########################################
AciI AluI AvaII BbvI BsmAI
BsrI Cac8I CfoI Cfr13I DpnII
Fnu4HI HaeIII HhaI HinP1I HinfI
HphI ItaI MaeIII MboI MnlI
MwoI NdeII NlaIII Sau3AI Sau96I
TaqI TfiI TseI TthHB8I XcmI