Restriction map for
pFN21AE6716
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# NO CUT ENZYMES ( 128 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
Alw44I AocI Aor51HI ApaI ApaLI
AscI Asp718I AviII BalI BamHI
BanI BanII BbeI BbrPI BbvCI
BciVI BclI BcnI BfrI BglI
BglII BsaAI BsaBI BsaHI BsaWI
BseAI BsiEI BsiWI BsmBI BspDI
BspEI BspLU11I BspMI BsrBI BsrDI
BssHII BssSI Bst1107I BstBI BstUI
BstZ17I Bsu36I CfoI ClaI CpoI
DsaI EaeI EagI EclXI Eco47III
Eco52I Eco81I EcoRV EcoT22I FauI
FbaI FseI FspI HaeII HhaI
Hin1I HinP1I HincII HindII HindIII
HpaI KasI KpnI KspI MamI
MfeI MluI MluNI MroI MscI
MunI MvnI NarI NciI NcoI
NdeI NheI NotI NruI NsiI
NspV PacI PaeR7I PflMI PinAI
PmaCI PmeI PmlI PshAI Psp1406I
PvuI RsrII SacI SacII SalI
SbfI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspI StuI SwaI Van91I XbaI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AciI 2220 GCGG CCGC(-3/-1)
AhdI 1185 GACTCTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AseI 1762 ATTAAT AT^TAAT
AsnI 1762 ATTAAT AT^TAAT
AspEI 1185 GACTCTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1753 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 2213 CCCGAG C^[CT]CG[GA]G
AvrII 469 CCTAGG C^CTAGG
BcgI 2162 GCAAGTGCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlnI 469 CCTAGG C^CTAGG
BlpI 124 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 124 GCTAAGC GC^T[ACGT]AGC
BsgI 1416 GTGCAG GTGCAG(16/14)
BsoBI 2213 CCCGAG C^[CT]CG[GA]G
Bsp1407I 1102 TGTACA T^GTACA
BspHI 1116 TCATGA T^CATGA
BsrFI 2252 GCCGGC [GA]^CCGG[CT]
BsrGI 1102 TGTACA T^GTACA
BstAPI 764 GCAAGGTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII 124 GCTAAGC GC^T[ACGT]AGC
Cfr10I 2252 GCCGGC [GA]^CCGG[CT]
DraI 1894 TTTAAA TTT^AAA
DraII 627 GGGACCC [GA]G^G[ACGT]CC[CT]
DraIII 1556 CACTCAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 2134 GACTGTGGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 1185 GACTCTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 1518 CCTAGATGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 627 GGGACCC [GA]G^G[ACGT]CC[CT]
EcoRI 372 GAATTC G^AATTC
HgaI 298 GACGC GACGC(5/10)
MaeII 205 ACGT A^CGT
NaeI 2252 GCCGGC GCC^GGC
NgoMIV 2252 GCCGGC G^CCGGC
PflFI 1753 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 627 GGGACCC [GA]G^G[AT]CC[CT]
PshBI 1762 ATTAAT AT^TAAT
PstI 390 CTGCAG CTGCA^G
RcaI 1116 TCATGA T^CATGA
SapI 187 GCTCTTC GCTCTTC(1/4)
SexAI 1754 ACCAGGT A^CC[AT]GGT
SfcI 390 CTGCAG C^T[GA][CT]AG
SmlI 1342 CTCAAG C^T[CT][GA]AG
SspBI 1102 TGTACA T^GTACA
TaqI 1968 TCGA T^CGA
Tth111I 1753 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 1968 TCGA T^CGA
XcmI 239 CCAAGTTCAATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 24 enzymes) #
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Asp700I 1970 GAATTATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2071 GAATTTCTTC
AspHI 610 GAGCAC G[AT]GC[AT]^C
2149 GTGCTC
BmyI 610 GAGCAC G[AGT]GC[ACT]^C
2149 GTGCTC
BsaI 829 GAGACC GGTCTC(1/5)
1940 GGTCTC
BsiHKAI 610 GAGCAC G[AT]GC[AT]^C
2149 GTGCTC
Bsp1286I 610 GAGCAC G[AGT]GC[ACT]^C
2149 GTGCTC
BstEII 552 GGTCACC G^GT[ACGT]ACC
1842 GGTAACC
BstPI 552 GGTCACC G^GT[ACGT]ACC
1842 GGTAACC
BstYI 739 AGATCC [GA]^GATC[CT]
1336 GGATCT
Cac8I 773 GCAAGC GC[ACGT]^[ACGT]GC
2252 GCCGGC
EarI 188 CTCTTC CTCTTC(1/4)
2223 GAAGAG
EcoO65I 552 GGTCACC G^GT[ACGT]ACC
1842 GGTAACC
HapII 607 CCGG C^CGG
2253 CCGG
HpaII 607 CCGG C^CGG
2253 CCGG
Ksp632I 188 CTCTTC CTCTTC(1/4)
2223 GAAGAG
MflI 739 AGATCC [GA]^GATC[CT]
1336 GGATCT
MspA1I 1492 CAGCTG C[AC]G^C[GT]G
1837 CAGCTG
MspI 607 CCGG C^CGG
2253 CCGG
NspI 1700 ACATGC [GA]CATG^[CT]
2207 ACATGC
PvuII 1492 CAGCTG CAG^CTG
1837 CAGCTG
ScaI 268 AGTACT AGT^ACT
2156 AGTACT
SfaNI 1789 GATGC GCATC(5/9)
906 GCATC
XhoII 739 AGATCC [GA]^GATC[CT]
1336 GGATCT
XmnI 1970 GAATTATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2071 GAATTTCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 59 enzymes) #
#########################################
AcsI AfaI AluI AlwI AlwNI
ApoI AvaII BbsI BbvI BfaI
BpmI BpuAI BsaJI BseRI BsiYI
BslI BsmAI BsmFI BsmI BsrI
BssKI BstF5I BstNI BstXI Cfr13I
DdeI DpnII EcoRII EcoT14I Fnu4HI
FokI HaeIII HinfI HphI ItaI
MaeI MaeIII MboI MboII MnlI
MseI MslI MvaI MwoI NdeII
NlaIII NlaIV PleI RsaI Sau3AI
Sau96I ScrFI StyI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI