Restriction map for
pFN21AE3672
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# NO CUT ENZYMES ( 135 enzymes) #
##################################
AatII Acc65I AccI AccIII AcyI
AflIII AgeI AhdI Alw44I AocI
Aor51HI ApaI ApaLI AscI Asp700I
Asp718I AspEI AvaI AviII AvrII
BalI BanI BanII BbeI BbrPI
BbvCI BclI BglII BlnI BsaAI
BsaBI BsaHI BsaWI BseAI BsgI
BsiEI BsiWI BsmBI BsoBI BspDI
BspEI BspLU11I BspMI BsrBI BsrDI
BsrFI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstXI BstZ17I Bsu36I
Cfr10I ClaI CpoI DraII DraIII
DrdI EaeI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO109I
EcoO65I EcoRV EcoT22I FbaI FseI
FspI HaeII HgaI Hin1I HincII
HindII HpaI KasI KpnI KspI
MamI MfeI MluI MluNI MroI
MscI MunI NaeI NarI NgoMIV
NotI NruI NsiI NspV PacI
PaeR7I PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI PvuI RsrII
SacI SacII SalI SbfI ScaI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspI
SwaI TaqI TthHB8I Van91I XbaI
XcmI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 63 enzymes) #
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AccII 858 CGCG CG^CG
AclI 688 AACGTT AA^CGTT
AfaI 116 GTAC GT^AC
AflII 1282 CTTAAG C^TTAAG
AlwNI 29 CAGCACCTG CAG[ACGT][ACGT][ACGT]^CTG
AseI 661 ATTAAT AT^TAAT
AsnI 661 ATTAAT AT^TAAT
AspHI 1582 GAGCAC G[AT]GC[AT]^C
AspI 1501 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbsI 3 GTCTTC GAAGAC(2/6)
BcgI 1352 GCAATTCAATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 232 GGATAC GTATCC(6/5)
BcnI 888 CCGGG CC^[GC]GG
BfrI 1282 CTTAAG C^TTAAG
BglI 1366 GCCCTAAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 1000 GCTAAGC GC^T[ACGT]AGC
BmyI 1582 GAGCAC G[AGT]GC[ACT]^C
Bpu1102I 1000 GCTAAGC GC^T[ACGT]AGC
BpuAI 3 GTCTTC GAAGAC(2/6)
BsaI 976 GAGACC GGTCTC(1/5)
BsiHKAI 1582 GAGCAC G[AT]GC[AT]^C
Bsp1286I 1582 GAGCAC G[AGT]GC[ACT]^C
Bsp1407I 115 TGTACA T^GTACA
BspHI 1289 TCATGA T^CATGA
BsrGI 115 TGTACA T^GTACA
BstUI 858 CGCG CG^CG
Cac8I 159 GCTAGC GC[ACGT]^[ACGT]GC
CelII 1000 GCTAAGC GC^T[ACGT]AGC
CfoI 859 GCGC GCG^C
DraI 594 TTTAAA TTT^AAA
DsaI 1040 CCATGG C^C[GA][CT]GG
EarI 752 GAAGAG CTCTTC(1/4)
FauI 1428 GCGGG CCCGC(4/6)
HapII 888 CCGG C^CGG
HhaI 859 GCGC GCG^C
HinP1I 859 GCGC G^CGC
HpaII 888 CCGG C^CGG
Ksp632I 752 GAAGAG CTCTTC(1/4)
MaeIII 505 GTGAC ^GT[ACGT]AC
MspA1I 220 CAGCTG C[AC]G^C[GT]G
MspI 888 CCGG C^CGG
MvnI 858 CGCG CG^CG
NciI 888 CCGGG CC^[GC]GG
NcoI 1040 CCATGG C^CATGG
NdeI 722 CATATG CA^TATG
NheI 159 GCTAGC G^CTAGC
NspI 818 ACATGC [GA]CATG^[CT]
PflFI 1501 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 612 GACTC GAGTC(4/5)
PshBI 661 ATTAAT AT^TAAT
Psp1406I 688 AACGTT AA^CGTT
PstI 447 CTGCAG CTGCA^G
PvuII 220 CAGCTG CAG^CTG
RcaI 1289 TCATGA T^CATGA
RsaI 116 GTAC GT^AC
SapI 752 GAAGAGC GCTCTTC(1/4)
SexAI 1502 ACCTGGT A^CC[AT]GGT
SfcI 447 CTGCAG C^T[GA][CT]AG
SmlI 1282 CTTAAG C^T[CT][GA]AG
SspBI 115 TGTACA T^GTACA
StuI 1600 AGGCCT AGG^CCT
Tsp45I 505 GTGAC ^GT[GC]AC
Tth111I 1501 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
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# TWO CUT ENZYMES ( 11 enzymes) #
###################################
BamHI 490 GGATCC G^GATCC
1483 GGATCC
BpmI 479 CTGGAG CTGGAG(16/14)
1070 CTGGAG
BseRI 970 GAGGAG GAGGAG(10/8)
973 GAGGAG
BsmFI 1235 GGGAC GGGAC(10/14)
1507 GTCCC
BstAPI 193 GCATCATCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
813 GCAAGACATGC
BstYI 490 GGATCC [GA]^GATC[CT]
1483 GGATCC
EcoRI 935 GAATTC G^AATTC
1293 GAATTC
HphI 678 GGTGA GGTGA(8/7)
1333 TCACC
MflI 490 GGATCC [GA]^GATC[CT]
1483 GGATCC
MslI 141 CACAGAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
705 CATGAGAATG
XhoII 490 GGATCC [GA]^GATC[CT]
1483 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 49 enzymes) #
#########################################
AciI AcsI AluI AlwI ApoI
AvaII BbvI BfaI BsaJI BsiYI
BslI BsmAI BsmI BsrI BssKI
BstF5I BstNI Cfr13I DdeI DpnII
EcoRII EcoT14I Fnu4HI FokI HaeIII
HindIII HinfI ItaI MaeI MaeII
MboI MboII MnlI MseI MvaI
MwoI NdeII NlaIII NlaIV Sau3AI
Sau96I ScrFI SfaNI StyI TfiI
Tru9I TseI Tsp509I TspRI