##################################
# NO CUT ENZYMES (  188 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcsI        AcyI        AfaI        AflII       
AflIII      AgeI        AhdI        Alw44I      AlwI        
AlwNI       Aor51HI     ApaI        ApaLI       ApoI        
AscI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AspI        AvaI        AvaII       AviII       
AvrII       BalI        BamHI       BanI        BbeI        
BbrPI       BbsI        BcgI        BciVI       BclI        
BcnI        BfaI        BfrI        BglI        BglII       
BlnI        BlpI        Bpu1102I    BpuAI       BsaAI       
BsaBI       BsaHI       BsaWI       BseAI       BsgI        
BsiWI       BsiYI       BslI        BsmBI       BsmFI       
BsmI        BsoBI       Bsp1407I    BspEI       BspLU11I    
BspMI       BsrBI       BsrDI       BsrFI       BsrGI       
BsrI        BssHII      BssSI       Bst1107I    BstAPI      
BstBI       BstEII      BstPI       BstUI       BstYI       
BstZ17I     CelII       Cfr10I      CpoI        DraI        
DraII       DraIII      DrdI        DsaI        Eam1105I    
Eco47III    EcoNI       EcoO109I    EcoO65I     EcoRI       
EcoRV       EcoT22I     FauI        FbaI        FseI        
FspI        HapII       HgaI        Hin1I       HincII      
HindII      HindIII     HinfI       HpaI        HpaII       
KasI        KpnI        KspI        MaeI        MaeII       
MaeIII      MamI        MfeI        MflI        MluI        
MluNI       MroI        MscI        MseI        MspI        
MunI        MvnI        NaeI        NarI        NciI        
NcoI        NdeI        NgoMIV      NheI        NlaIV       
NotI        NruI        NsiI        NspV        PacI        
PaeR7I      PflFI       PflMI       PinAI       PleI        
PmaCI       PmeI        PmlI        PpuMI       PshAI       
PshBI       Psp1406I    PvuI        RsaI        RsrII       
SacII       SalI        SbfI        ScaI        SexAI       
SfiI        SfoI        SfuI        SgrAI       SmaI        
SmlI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        StuI        SwaI        TfiI        
Tru9I       Tsp45I      Tsp509I     TspRI       Tth111I     
Van91I      XbaI        XcmI        XhoI        XhoII       
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   40 enzymes) #
###################################

AocI          160  CCTGAGG              CC^T[ACGT]AGG
AspHI         403  GAGCTC               G[AT]GC[AT]^C
BanII         403  GAGCTC               G[GA]GC[CT]^C
BbvCI          91  GCTGAGG              CCTCAGC(-5/-2)
BmyI          403  GAGCTC               G[AGT]GC[ACT]^C
BpmI          460  CTGGAG               CTGGAG(16/14)
BsaI          376  GAGACC               GGTCTC(1/5)
BsaJI         113  CCAAGG               C^C[ACGT][ACGT]GG
BsiEI         435  CGGCCG               CG[GA][CT]^CG
BsiHKAI       403  GAGCTC               G[AT]GC[AT]^C
Bsp1286I      403  GAGCTC               G[AGT]GC[ACT]^C
BspDI         181  ATCGAT               AT^CGAT
BstXI         113  CCAAGGAGCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I        160  CCTGAGG              CC^T[ACGT]AGG
Cac8I         363  GCAGGC               GC[ACGT]^[ACGT]GC
CfoI           89  GCGC                 GCG^C
Cfr13I        144  GGGCC                G^G[ACGT]CC
ClaI          181  ATCGAT               AT^CGAT
DpnII         180  GATC                 ^GATC
EaeI          435  CGGCCG               [CT]^GGCC[GA]
EagI          435  CGGCCG               C^GGCCG
EclXI         435  CGGCCG               C^GGCCG
Eco52I        435  CGGCCG               C^GGCCG
Eco81I        160  CCTGAGG              CC^T[ACGT]AGG
EcoT14I       113  CCAAGG               C^C[AT][AT]GG
HaeII          88  GGCGCT               [GA]GCGC^[CT]
HhaI           89  GCGC                 GCG^C
HinP1I         89  GCGC                 G^CGC
HphI          129  GGTGA                GGTGA(8/7)
MboI          180  GATC                 ^GATC
MslI           75  CATCTTCGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MwoI          101  GCTGCATCAGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NdeII         180  GATC                 ^GATC
NspI          305  GCATGT               [GA]CATG^[CT]
PvuII          31  CAGCTG               CAG^CTG
SacI          403  GAGCTC               GAGCT^C
SapI           40  GAAGAGC              GCTCTTC(1/4)
Sau3AI        180  GATC                 ^GATC
Sau96I        144  GGGCC                G^G[ACGT]CC
StyI          113  CCAAGG               C^C[AT][AT]GG


###################################
# TWO CUT ENZYMES (    6 enzymes) #
###################################

BsmAI         376  GAGAC                GTCTC(1/5)
              419  GAGAC               
EarI          298  CTCTTC               CTCTTC(1/4)
               40  GAAGAG              
HaeIII        145  GGCC                 GG^CC
              436  GGCC                
Ksp632I       298  CTCTTC               CTCTTC(1/4)
               40  GAAGAG              
MspA1I         31  CAGCTG               C[AC]G^C[GT]G
              204  CAGCGG              
PstI          169  CTGCAG               CTGCA^G
              361  CTGCAG              


#########################################
# 3 OR MORE CUT ENZYMES (   24 enzymes) #
#########################################

AciI        AluI        BbvI        BseRI       BspHI       
BssKI       BstF5I      BstNI       DdeI        EcoRII      
Fnu4HI      FokI        ItaI        MboII       MnlI        
MvaI        NlaIII      RcaI        ScrFI       SfaNI       
SfcI        TaqI        TseI        TthHB8I