Restriction map for
pFN21AE1480
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# NO CUT ENZYMES ( 190 enzymes) #
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AatII Acc65I AccI AccII AclI
AcsI AcyI AflII AflIII AgeI
AhdI AluI Alw44I AlwNI AocI
ApaI ApaLI ApoI AscI AseI
AsnI Asp718I AspEI AspHI AspI
AvaII AviII AvrII BamHI BanI
BanII BbeI BbrPI BbvCI BcgI
BciVI BclI BfrI BglI BglII
BlnI BlpI BmyI BpmI Bpu1102I
BsaAI BsaBI BsaHI BsaI BseRI
BsgI BsiEI BsiHKAI BsiWI BsmBI
BsmI Bsp1286I Bsp1407I BspDI BspHI
BspLU11I BspMI BsrBI BsrFI BsrGI
BssHII Bst1107I BstAPI BstBI BstEII
BstF5I BstPI BstUI BstXI BstYI
BstZ17I Bsu36I CelII Cfr10I Cfr13I
ClaI CpoI DraI DraII DraIII
DrdI DsaI EagI Eam1105I EarI
EclXI Eco52I Eco81I EcoNI EcoO109I
EcoO65I EcoRI EcoRV EcoT14I EcoT22I
FauI FbaI FokI FseI FspI
Hin1I HincII HindII HindIII HinfI
HpaI KasI KpnI Ksp632I KspI
MaeII MamI MfeI MflI MluI
MseI MslI MunI MvnI NaeI
NarI NcoI NdeI NgoMIV NheI
NlaIV NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PflMI
PinAI PleI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PstI
PvuI PvuII RcaI RsrII SacI
SacII SalI SapI Sau96I SbfI
ScaI SexAI SfaNI SfcI SfiI
SfoI SfuI SgrAI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI StyI SwaI TaqI TfiI
Tru9I Tsp45I Tsp509I Tth111I TthHB8I
Van91I XbaI XcmI XhoI XhoII
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# ONE CUT ENZYMES ( 49 enzymes) #
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AccIII 28 TCCGGA T^CCGGA
AciI 124 GCGG CCGC(-3/-1)
AfaI 84 GTAC GT^AC
AlwI 200 GATCC GGATC(4/5)
Aor51HI 150 AGCGCT AGC^GCT
Asp700I 250 GAAAATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 105 CCCGGG C^[CT]CG[GA]G
BalI 134 TGGCCA TGG^CCA
BbsI 274 GAAGAC GAAGAC(2/6)
BbvI 153 GCTGC GCAGC(8/12)
BfaI 4 CTAG C^TAG
BpuAI 274 GAAGAC GAAGAC(2/6)
BsaWI 28 TCCGGA [AT]^CCGG[AT]
BseAI 28 TCCGGA T^CCGGA
BsmAI 211 GAGAC GTCTC(1/5)
BsmFI 48 GTCCC GGGAC(10/14)
BsoBI 105 CCCGGG C^[CT]CG[GA]G
BspEI 28 TCCGGA T^CCGGA
BsrDI 283 GCAATG GCAATG(2/0)
BsrI 18 ACTGG ACTGG(1/-1)
BssSI 55 CTCGTG CACGAG(-5/-1)
Cac8I 72 GCTGGC GC[ACGT]^[ACGT]GC
DdeI 147 CTCAG C^T[ACGT]AG
DpnII 200 GATC ^GATC
Eco47III 150 AGCGCT AGC^GCT
Fnu4HI 153 GCTGC GC^[ACGT]GC
HaeII 150 AGCGCT [GA]GCGC^[CT]
HgaI 115 GACGC GACGC(5/10)
HphI 176 TCACC GGTGA(8/7)
ItaI 153 GCTGC GC^[ACGT]GC
MaeI 4 CTAG C^TAG
MaeIII 8 GTAAC ^GT[ACGT]AC
MboI 200 GATC ^GATC
MboII 274 GAAGA GAAGA(8/7)
MluNI 134 TGGCCA TGG^CCA
MroI 28 TCCGGA T^CCGGA
MscI 134 TGGCCA TGG^CCA
MspA1I 122 CAGCGG C[AC]G^C[GT]G
MwoI 15 GCGACTGGGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NdeII 200 GATC ^GATC
NlaIII 132 CATG CATG^
RsaI 84 GTAC GT^AC
Sau3AI 200 GATC ^GATC
SmaI 105 CCCGGG CCC^GGG
TseI 153 GCTGC G^C[AT]GC
TspRI 215 CACTG CA[GC]TG[ACGT][ACGT]^
XmaCI 105 CCCGGG C^CCGGG
XmaI 105 CCCGGG C^CCGGG
XmnI 250 GAAAATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 12 enzymes) #
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BcnI 105 CCCGG CC^[GC]GG
106 CCGGG
BsiYI 105 CCCGGGCGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
260 CCAGAACTCGG
BslI 105 CCCGGGCGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
260 CCAGAACTCGG
BstNI 63 CCTGG CC^[AT]GG
179 CCAGG
CfoI 70 GCGC GCG^C
151 GCGC
EaeI 74 TGGCCG [CT]^GGCC[GA]
134 TGGCCA
EcoRII 63 CCTGG ^CC[AT]GG
179 CCAGG
HaeIII 75 GGCC GG^CC
135 GGCC
HhaI 70 GCGC GCG^C
151 GCGC
HinP1I 70 GCGC G^CGC
151 GCGC
MvaI 63 CCTGG CC^[AT]GG
179 CCAGG
NciI 105 CCCGG CC^[GC]GG
106 CCGGG
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# 3 OR MORE CUT ENZYMES ( 7 enzymes) #
#########################################
BsaJI BssKI HapII HpaII MnlI
MspI ScrFI