##################################
# NO CUT ENZYMES (  217 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AciI        AclI        AcsI        AcyI        AfaI        
AflII       AflIII      AgeI        AhdI        Alw44I      
AlwNI       AocI        Aor51HI     ApaI        ApaLI       
ApoI        AscI        AseI        AsnI        Asp700I     
Asp718I     AspEI       AspI        AvaI        AviII       
AvrII       BalI        BanI        BbeI        BbrPI       
BbsI        BbvCI       BbvI        BcgI        BciVI       
BclI        BcnI        BfrI        BglI        BglII       
BlnI        BlpI        BpmI        Bpu1102I    BpuAI       
BsaAI       BsaBI       BsaHI       BsaI        BsaJI       
BsaWI       BseAI       BseRI       BsgI        BsiEI       
BsiWI       BsiYI       BslI        BsmAI       BsmBI       
BsmFI       BsoBI       Bsp1407I    BspDI       BspEI       
BspHI       BspLU11I    BspMI       BsrBI       BsrDI       
BsrFI       BsrGI       BssHII      BssKI       BssSI       
Bst1107I    BstAPI      BstBI       BstEII      BstNI       
BstPI       BstUI       BstXI       BstZ17I     Bsu36I      
Cac8I       CelII       CfoI        Cfr10I      ClaI        
CpoI        DdeI        DraI        DraIII      DrdI        
DsaI        EaeI        EagI        Eam1105I    EarI        
EclXI       Eco47III    Eco52I      Eco81I      EcoNI       
EcoO65I     EcoRI       EcoRII      EcoRV       EcoT14I     
EcoT22I     FauI        FbaI        Fnu4HI      FseI        
FspI        HaeII       HaeIII      HapII       HgaI        
HhaI        Hin1I       HinP1I      HindIII     HinfI       
HpaI        HpaII       HphI        ItaI        KasI        
KpnI        Ksp632I     KspI        MamI        MfeI        
MluI        MluNI       MroI        MscI        MseI        
MslI        MspA1I      MspI        MunI        MvaI        
MvnI        MwoI        NaeI        NarI        NciI        
NcoI        NdeI        NgoMIV      NheI        NlaIII      
NotI        NruI        NsiI        NspI        NspV        
PacI        PaeR7I      PflFI       PflMI       PinAI       
PleI        PmaCI       PmeI        PmlI        PshAI       
PshBI       Psp1406I    PstI        PvuI        PvuII       
RcaI        RsaI        RsrII       SacII       SalI        
SapI        SbfI        ScaI        ScrFI       SexAI       
SfcI        SfiI        SfoI        SfuI        SgrAI       
SmaI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       StuI        StyI        SwaI        TaqI        
TfiI        Tru9I       TseI        Tth111I     TthHB8I     
Van91I      XbaI        XcmI        XhoI        XmaCI       
XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   31 enzymes) #
###################################

AspHI         196  GAGCTC               G[AT]GC[AT]^C
AvaII          21  GGACC                G^G[AT]CC
BamHI         150  GGATCC               G^GATCC
BanII         196  GAGCTC               G[GA]GC[CT]^C
BfaI           94  CTAG                 C^TAG
BmyI          196  GAGCTC               G[AGT]GC[ACT]^C
BsiHKAI       196  GAGCTC               G[AT]GC[AT]^C
BsmI           89  GAATGC               GAATGC(1/-1)
Bsp1286I      196  GAGCTC               G[AGT]GC[ACT]^C
BstF5I        168  CATCC                GGATG(2/0)
BstYI         150  GGATCC               [GA]^GATC[CT]
Cfr13I         21  GGACC                G^G[ACGT]CC
DraII          20  AGGACCT              [GA]G^G[ACGT]CC[CT]
EcoO109I       20  AGGACCT              [GA]G^G[ACGT]CC[CT]
FokI          168  CATCC                GGATG(9/13)
HincII         55  GTTGAC               GT[CT]^[GA]AC
HindII         55  GTTGAC               GT[CT]^[GA]AC
MaeI           94  CTAG                 C^TAG
MaeII         128  ACGT                 A^CGT
MaeIII         76  GTGAC                ^GT[ACGT]AC
MflI          150  GGATCC               [GA]^GATC[CT]
NlaIV         150  GGATCC               GG[ACGT]^[ACGT]CC
PpuMI          20  AGGACCT              [GA]G^G[AT]CC[CT]
SacI          196  GAGCTC               GAGCT^C
Sau96I         21  GGACC                G^G[ACGT]CC
SfaNI         167  GCATC                GCATC(5/9)
SmlI           64  CTCAAG               C^T[CT][GA]AG
SspI           13  AATATT               AAT^ATT
Tsp45I         76  GTGAC                ^GT[GC]AC
Tsp509I        41  AATT                 ^AATT
XhoII         150  GGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (    7 enzymes) #
###################################

AluI           62  AGCT                 AG^CT
              197  AGCT                
AlwI          151  GATCC                GGATC(4/5)
              150  GGATC               
DpnII         140  GATC                 ^GATC
              151  GATC                
MboI          140  GATC                 ^GATC
              151  GATC                
NdeII         140  GATC                 ^GATC
              151  GATC                
Sau3AI        140  GATC                 ^GATC
              151  GATC                
TspRI          80  CACTG                CA[GC]TG[ACGT][ACGT]^
              155  CACTG               


#########################################
# 3 OR MORE CUT ENZYMES (    3 enzymes) #
#########################################

BsrI        MboII       MnlI