Restriction map for
pFN21AE4403
##################################
# NO CUT ENZYMES ( 120 enzymes) #
##################################
AatII AccI AccIII AclI AcsI
AcyI AflII AgeI Alw44I AlwNI
AocI Aor51HI ApaI ApaLI ApoI
AscI AseI AsnI Asp700I AspHI
BanII BbeI BbrPI BbvCI BcgI
BclI BfrI BglI BglII BsaHI
BsaI BsaWI BseAI BsiHKAI BsiWI
BsmAI BsmBI BsmI Bsp1407I BspEI
BspHI BsrBI BsrGI BssHII BssSI
Bst1107I BstEII BstPI BstZ17I Bsu36I
CpoI DraI DraIII DrdI EagI
EarI EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoRI EcoRV FbaI
FseI HgaI Hin1I HincII HindII
HpaI KasI Ksp632I MfeI MluI
MroI MunI NaeI NarI NdeI
NgoMIV NheI NotI NruI PacI
PaeR7I PflMI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PstI
PvuI PvuII RcaI RsrII SacI
SalI SapI SbfI ScaI SfcI
SfiI SfoI SgrAI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI Van91I XbaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
Acc65I 86 GGTACC G^GTACC
AccII 1086 CGCG CG^CG
AflIII 709 ACATGT A^C[GA][CT]GT
AhdI 334 GACGAGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp718I 86 GGTACC G^GTACC
AspEI 334 GACGAGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 370 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 995 GGATCC G^GATCC
BbsI 483 GTCTTC GAAGAC(2/6)
BciVI 934 GGATAC GTATCC(6/5)
BmyI 63 GGGCAC G[AGT]GC[ACT]^C
BpuAI 483 GTCTTC GAAGAC(2/6)
BsaAI 475 TACGTG [CT]AC^GT[GA]
BsaBI 1155 GATAGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsgI 537 CTGCAC GTGCAG(16/14)
BsiEI 1088 CGGTCG CG[GA][CT]^CG
Bsp1286I 63 GGGCAC G[AGT]GC[ACT]^C
BspLU11I 709 ACATGT A^CATGT
BsrDI 35 GCAATG GCAATG(2/0)
BsrFI 98 ACCGGC [GA]^CCGG[CT]
BstAPI 471 GCATTACGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 954 TTCGAA TT^CGAA
BstUI 1086 CGCG CG^CG
BstXI 1053 CCAGAAGCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 98 ACCGGC [GA]^CCGG[CT]
Eam1105I 334 GACGAGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoT22I 437 ATGCAT ATGCA^T
FauI 208 CCCGC CCCGC(4/6)
HaeII 926 AGCGCC [GA]GCGC^[CT]
HindIII 223 AAGCTT A^AGCTT
KpnI 86 GGTACC GGTAC^C
KspI 1085 CCGCGG CCGC^GG
MamI 1155 GATAGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MboII 484 TCTTC GAAGA(8/7)
MslI 757 CATAATTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 1086 CGCG CG^CG
NcoI 59 CCATGG C^CATGG
NsiI 437 ATGCAT ATGCA^T
NspI 709 ACATGT [GA]CATG^[CT]
NspV 954 TTCGAA TT^CGAA
PflFI 370 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
SacII 1085 CCGCGG CCGC^GG
SexAI 1178 ACCTGGT A^CC[AT]GGT
SfuI 954 TTCGAA TT^CGAA
SmaI 542 CCCGGG CCC^GGG
SmlI 595 CTCAAG C^T[CT][GA]AG
Tth111I 370 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 575 CCATCACTGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 542 CCCGGG C^CCGGG
XmaI 542 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AfaI 87 GTAC GT^AC
1101 GTAC
AvaI 488 CTCGGG C^[CT]CG[GA]G
542 CCCGGG
AviII 170 TGCGCA TGC^GCA
494 TGCGCA
AvrII 133 CCTAGG C^CTAGG
930 CCTAGG
BfaI 134 CTAG C^TAG
931 CTAG
BlnI 133 CCTAGG C^CTAGG
930 CCTAGG
BlpI 519 GCTGAGC GC^T[ACGT]AGC
1005 GCTCAGC
Bpu1102I 519 GCTGAGC GC^T[ACGT]AGC
1005 GCTCAGC
BseRI 337 GAGGAG GAGGAG(10/8)
793 GAGGAG
BsmFI 602 GGGAC GGGAC(10/14)
696 GTCCC
BsoBI 488 CTCGGG C^[CT]CG[GA]G
542 CCCGGG
BspDI 664 ATCGAT AT^CGAT
1162 ATCGAT
BspMI 89 ACCTGC ACCTGC(4/8)
167 ACCTGC
CelII 519 GCTGAGC GC^T[ACGT]AGC
1005 GCTCAGC
ClaI 664 ATCGAT AT^CGAT
1162 ATCGAT
FspI 170 TGCGCA TGC^GCA
494 TGCGCA
MaeI 134 CTAG C^TAG
931 CTAG
MspA1I 655 CAGCGG C[AC]G^C[GT]G
1085 CCGCGG
PpuMI 602 GGGACCT [GA]G^G[AT]CC[CT]
1013 AGGACCC
RsaI 87 GTAC GT^AC
1101 GTAC
TfiI 749 GAATC G^A[AT]TC
857 GATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 67 enzymes) #
#########################################
AciI AluI AlwI AvaII BalI
BanI BbvI BcnI BpmI BsaJI
BsiYI BslI BsrI BssKI BstF5I
BstNI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
EcoO109I EcoRII EcoT14I Fnu4HI FokI
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI MaeII MaeIII
MboI MflI MluNI MnlI MscI
MseI MspI MvaI MwoI NciI
NdeII NlaIII NlaIV PleI Sau3AI
Sau96I ScrFI SfaNI StyI TaqI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII