Restriction map for
pFN21AE6233
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# NO CUT ENZYMES ( 148 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AcyI AgeI AhdI Alw44I AocI
Aor51HI ApaI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AvaI AviII AvrII BbeI
BcgI BciVI BclI BglI BglII
BlnI BlpI Bpu1102I BsaBI BsaHI
BsaI BseAI BsiEI BsiWI BsmI
BsoBI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrBI BsrDI BsrFI BsrGI
BssHII BssSI Bst1107I BstBI BstXI
BstZ17I Bsu36I CelII Cfr10I ClaI
CpoI DraI DraII DraIII DrdI
DsaI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO109I EcoRI
EcoRV EcoT14I EcoT22I FbaI FseI
FspI HaeII HgaI Hin1I HincII
HindII HindIII HpaI KasI KpnI
KspI MamI MfeI MluI MroI
MslI MunI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PinAI PmeI PpuMI PshAI
PshBI Psp1406I PvuI PvuII RcaI
RsrII SacII SalI SbfI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SspI StyI SwaI TaqI TfiI
Tth111I TthHB8I XbaI XcmI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AccI 289 GTCTAC GT^[AC][GT]AC
AflII 249 CTTAAG C^TTAAG
AflIII 25 ACGTGT A^C[GA][CT]GT
BalI 30 TGGCCA TGG^CCA
BamHI 498 GGATCC G^GATCC
BanI 667 GGCACC G^G[CT][GA]CC
BbrPI 805 CACGTG CAC^GTG
BbsI 21 GAAGAC GAAGAC(2/6)
BbvCI 769 GCTGAGG CCTCAGC(-5/-2)
BfaI 349 CTAG C^TAG
BfrI 249 CTTAAG C^TTAAG
BpmI 362 CTCCAG CTGGAG(16/14)
BpuAI 21 GAAGAC GAAGAC(2/6)
BsaWI 210 ACCGGA [AT]^CCGG[AT]
BsmAI 298 GTCTC GTCTC(1/5)
BsmBI 297 CGTCTC CGTCTC(1/5)
BsmFI 102 GTCCC GGGAC(10/14)
BspMI 642 GCAGGT ACCTGC(4/8)
BsrI 154 ACTGG ACTGG(1/-1)
BstAPI 383 GCATCAACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 498 GGATCC [GA]^GATC[CT]
EarI 160 CTCTTC CTCTTC(1/4)
FauI 620 CCCGC CCCGC(4/6)
HinfI 333 GACTC G^A[ACGT]TC
Ksp632I 160 CTCTTC CTCTTC(1/4)
MaeI 349 CTAG C^TAG
MflI 498 GGATCC [GA]^GATC[CT]
MluNI 30 TGGCCA TGG^CCA
MscI 30 TGGCCA TGG^CCA
MspA1I 637 CCGCTG C[AC]G^C[GT]G
PflMI 33 CCAGCGCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 333 GACTC GAGTC(4/5)
PmaCI 805 CACGTG CAC^GTG
PmlI 805 CACGTG CAC^GTG
SacI 625 GAGCTC GAGCT^C
SapI 159 GCTCTTC GCTCTTC(1/4)
Tsp509I 285 AATT ^AATT
TspRI 194 CAGTG CA[GC]TG[ACGT][ACGT]^
Van91I 33 CCAGCGCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 498 GGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AccII 427 CGCG CG^CG
622 CGCG
AspHI 625 GAGCTC G[AT]GC[AT]^C
802 GAGCAC
AvaII 198 GGACC G^G[AT]CC
612 GGACC
BanII 157 GGGCTC G[GA]GC[CT]^C
625 GAGCTC
BcnI 69 CCCGG CC^[GC]GG
104 CCCGG
BseRI 748 CTCCTC GAGGAG(10/8)
43 GAGGAG
BsgI 390 CTGCAC GTGCAG(16/14)
679 CTGCAC
BsiHKAI 625 GAGCTC G[AT]GC[AT]^C
802 GAGCAC
BstEII 233 GGTCACC G^GT[ACGT]ACC
243 GGTAACC
BstF5I 756 CATCC GGATG(2/0)
258 GGATG
BstPI 233 GGTCACC G^GT[ACGT]ACC
243 GGTAACC
BstUI 427 CGCG CG^CG
622 CGCG
DdeI 486 CTCAG C^T[ACGT]AG
770 CTGAG
EaeI 30 TGGCCA [CT]^GGCC[GA]
630 CGGCCA
EcoO65I 233 GGTCACC G^GT[ACGT]ACC
243 GGTAACC
FokI 756 CATCC GGATG(9/13)
258 GGATG
HphI 147 GGTGA GGTGA(8/7)
235 TCACC
MseI 250 TTAA T^TAA
542 TTAA
MvnI 427 CGCG CG^CG
622 CGCG
NciI 69 CCCGG CC^[GC]GG
104 CCCGG
ScaI 404 AGTACT AGT^ACT
650 AGTACT
SmlI 249 CTTAAG C^T[CT][GA]AG
859 CTCAAG
StuI 276 AGGCCT AGG^CCT
489 AGGCCT
Tru9I 250 TTAA T^TAA
542 TTAA
Tsp45I 234 GTCAC ^GT[GC]AC
808 GTGAC
#########################################
# 3 OR MORE CUT ENZYMES ( 45 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
BbvI BmyI BsaAI BsaJI BsiYI
BslI Bsp1286I BssKI BstNI Cac8I
CfoI Cfr13I DpnII EcoRII Fnu4HI
HaeIII HapII HhaI HinP1I HpaII
ItaI MaeII MaeIII MboI MboII
MnlI MspI MvaI MwoI NdeII
NlaIII NlaIV PstI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI TseI