Restriction map for
pFN21AE9459
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# NO CUT ENZYMES ( 114 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AflII AocI ApoI AscI AseI
AsnI Asp700I Asp718I AviII AvrII
BamHI BbeI BbrPI BciVI BfaI
BfrI BlnI BlpI Bpu1102I BsaAI
BsaBI BsaI BseAI BsiWI BspDI
BspEI BspHI BspLU11I BsrBI BssHII
BssSI Bst1107I BstAPI BstBI BstZ17I
Bsu36I CelII ClaI CpoI DraI
DraIII DrdI EagI EclXI Eco52I
Eco81I EcoNI EcoRI EcoRV EcoT22I
FauI FseI FspI HincII HindII
HpaI KasI KpnI KspI MaeI
MamI MfeI MluI MroI MseI
MunI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflMI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacII SalI
SbfI ScaI SfiI SfoI SfuI
SgrAI SnaBI SpeI Sse8387I SspI
StuI SwaI Tru9I Tsp509I Van91I
XbaI XcmI XhoI XmnI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AccII 637 CGCG CG^CG
AcyI 73 GGCGTC G[GA]^CG[CT]C
AflIII 1232 ACACGT A^C[GA][CT]GT
AgeI 1025 ACCGGT A^CCGGT
AhdI 1228 GACTACACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 664 GTGCAC G^TGCAC
Aor51HI 946 AGCGCT AGC^GCT
ApaI 849 GGGCCC GGGCC^C
ApaLI 664 GTGCAC G^TGCAC
AspEI 1228 GACTACACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 207 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbsI 196 GTCTTC GAAGAC(2/6)
BcgI 185 GCAACAAGTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 326 TGATCA T^GATCA
BglII 578 AGATCT A^GATCT
BpuAI 196 GTCTTC GAAGAC(2/6)
BsaHI 73 GGCGTC G[GA]^CG[CT]C
BsaWI 1025 ACCGGT [AT]^CCGG[AT]
BsgI 1260 CTGCAC GTGCAG(16/14)
BsiEI 369 CGACCG CG[GA][CT]^CG
BsmBI 900 CGTCTC CGTCTC(1/5)
BsmI 759 GAATGC GAATGC(1/-1)
Bsp1407I 155 TGTACA T^GTACA
BsrDI 360 CATTGC GCAATG(2/0)
BsrFI 1025 ACCGGT [GA]^CCGG[CT]
BsrGI 155 TGTACA T^GTACA
BstUI 637 CGCG CG^CG
BstXI 812 CCATCCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 1025 ACCGGT [GA]^CCGG[CT]
Eam1105I 1228 GACTACACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 946 AGCGCT AGC^GCT
FbaI 326 TGATCA T^GATCA
HaeII 946 AGCGCT [GA]GCGC^[CT]
HgaI 74 GCGTC GACGC(5/10)
Hin1I 73 GGCGTC G[GA]^CG[CT]C
HindIII 1392 AAGCTT A^AGCTT
MaeII 1234 ACGT A^CGT
MvnI 637 CGCG CG^CG
NcoI 395 CCATGG C^CATGG
PflFI 207 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 1025 ACCGGT A^CCGGT
SapI 1071 GCTCTTC GCTCTTC(1/4)
SexAI 134 ACCTGGT A^CC[AT]GGT
SmaI 1482 CCCGGG CCC^GGG
SmlI 1222 CTTGAG C^T[CT][GA]AG
SphI 341 GCATGC GCATG^C
SspBI 155 TGTACA T^GTACA
TfiI 1341 GATTC G^A[AT]TC
Tth111I 207 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 1482 CCCGGG C^CCGGG
XmaI 1482 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AccI 445 GTCTAC GT^[AC][GT]AC
778 GTCTAC
AlwI 278 GGATC GGATC(4/5)
1091 GGATC
AlwNI 1144 CAGCCCCTG CAG[ACGT][ACGT][ACGT]^CTG
1531 CAGAGGCTG
BalI 641 TGGCCA TGG^CCA
1397 TGGCCA
BanI 545 GGCACC G^G[CT][GA]CC
829 GGTGCC
BbvCI 18 CCTCAGC CCTCAGC(-5/-2)
180 CCTCAGC
BpmI 723 CTCCAG CTGGAG(16/14)
913 CTGGAG
BsmAI 916 GAGAC GTCTC(1/5)
901 GTCTC
BspMI 320 ACCTGC ACCTGC(4/8)
1135 GCAGGT
BstEII 147 GGTCACC G^GT[ACGT]ACC
840 GGTCACC
BstPI 147 GGTCACC G^GT[ACGT]ACC
840 GGTCACC
BstYI 278 GGATCT [GA]^GATC[CT]
578 AGATCT
CfoI 947 GCGC GCG^C
1126 GCGC
DsaI 395 CCATGG C^C[GA][CT]GG
503 CCGTGG
EcoO65I 147 GGTCACC G^GT[ACGT]ACC
840 GGTCACC
EcoT14I 395 CCATGG C^C[AT][AT]GG
1373 CCTTGG
HhaI 947 GCGC GCG^C
1126 GCGC
HinP1I 947 GCGC G^CGC
1126 GCGC
MflI 278 GGATCT [GA]^GATC[CT]
578 AGATCT
MluNI 641 TGGCCA TGG^CCA
1397 TGGCCA
MscI 641 TGGCCA TGG^CCA
1397 TGGCCA
MslI 609 CATGCTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
813 CATCCTGGTG
MspA1I 21 CAGCTG C[AC]G^C[GT]G
1185 CAGCTG
NspI 341 GCATGC [GA]CATG^[CT]
1010 GCATGT
PstI 24 CTGCAG CTGCA^G
1537 CTGCAG
PvuII 21 CAGCTG CAG^CTG
1185 CAGCTG
SacI 258 GAGCTC GAGCT^C
1284 GAGCTC
SfcI 24 CTGCAG C^T[GA][CT]AG
1537 CTGCAG
StyI 395 CCATGG C^C[AT][AT]GG
1373 CCTTGG
XhoII 278 GGATCT [GA]^GATC[CT]
578 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 63 enzymes) #
#########################################
AciI AfaI AluI AspHI AvaI
AvaII BanII BbvI BcnI BglI
BmyI BsaJI BseRI BsiHKAI BsiYI
BslI BsmFI BsoBI Bsp1286I BsrI
BssKI BstF5I BstNI Cac8I Cfr13I
DdeI DpnII DraII EaeI EarI
EcoO109I EcoRII Fnu4HI FokI HaeIII
HapII HinfI HpaII HphI ItaI
Ksp632I MaeIII MboI MboII MnlI
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV PleI RsaI Sau3AI
Sau96I ScrFI SfaNI TaqI TseI
Tsp45I TspRI TthHB8I