Restriction map for
pFN21AE5613
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# NO CUT ENZYMES ( 115 enzymes) #
##################################
AatII Acc65I AccI AclI AcsI
AflII AflIII AgeI AhdI AocI
Aor51HI ApoI AseI AsnI Asp718I
AspEI AviII AvrII BalI BamHI
BbrPI BbvCI BciVI BclI BfaI
BfrI BglII BlnI BlpI Bpu1102I
BsaAI BsaBI BsaI BsiWI BsmBI
BsmI Bsp1407I BspDI BspHI BspLU11I
BsrGI BsrI Bst1107I BstBI BstEII
BstPI BstZ17I Bsu36I CelII ClaI
CpoI DraI DraIII DrdI Eam1105I
Eco47III Eco81I EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI FspI HincII
HindII HindIII HpaI KpnI MaeI
MamI MfeI MluI MluNI MscI
MseI MunI NcoI NdeI NheI
NotI NruI NsiI NspV PacI
PaeR7I PflMI PinAI PmaCI PmeI
PmlI PshBI Psp1406I PvuI RcaI
RsrII SalI SbfI ScaI SexAI
SfiI SfuI SgrAI SnaBI SpeI
Sse8387I SspBI SspI StuI SwaI
Tru9I Tsp509I Van91I XbaI XhoI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AccIII 1081 TCCGGA T^CCGGA
Alw44I 486 GTGCAC G^TGCAC
AlwNI 43 CAGCTCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 82 GGGCCC GGGCC^C
ApaLI 486 GTGCAC G^TGCAC
AscI 24 GGCGCGCC GG^CGCGCC
AspI 699 GACGCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbeI 841 GGCGCC GGCGC^C
BbsI 327 GAAGAC GAAGAC(2/6)
BcgI 1319 GCAAGTGTTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BpmI 612 CTCCAG CTGGAG(16/14)
BpuAI 327 GAAGAC GAAGAC(2/6)
BseAI 1081 TCCGGA T^CCGGA
BsiEI 204 CGGCCG CG[GA][CT]^CG
BspEI 1081 TCCGGA T^CCGGA
BspMI 964 GCAGGT ACCTGC(4/8)
BsrDI 713 CATTGC GCAATG(2/0)
BsrFI 29 GCCGGC [GA]^CCGG[CT]
BssHII 25 GCGCGC G^CGCGC
BstAPI 42 GCAGCTCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 152 AGATCC [GA]^GATC[CT]
Cfr10I 29 GCCGGC [GA]^CCGG[CT]
EagI 204 CGGCCG C^GGCCG
EclXI 204 CGGCCG C^GGCCG
Eco52I 204 CGGCCG C^GGCCG
EcoNI 1218 CCTCCCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 1286 CCAAGG C^C[AT][AT]GG
KasI 841 GGCGCC G^GCGCC
MaeII 446 ACGT A^CGT
MflI 152 AGATCC [GA]^GATC[CT]
MroI 1081 TCCGGA T^CCGGA
NaeI 29 GCCGGC GCC^GGC
NarI 841 GGCGCC GG^CGCC
NgoMIV 29 GCCGGC G^CCGGC
NspI 665 GCATGC [GA]CATG^[CT]
PflFI 699 GACGCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 765 GACAACAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 217 GAGCTC GAGCT^C
SapI 189 GCTCTTC GCTCTTC(1/4)
SfoI 841 GGCGCC GGC^GCC
SmlI 181 CTCAAG C^T[CT][GA]AG
SphI 665 GCATGC GCATG^C
StyI 1286 CCAAGG C^C[AT][AT]GG
TfiI 289 GATTC G^A[AT]TC
Tsp45I 824 GTCAC ^GT[GC]AC
Tth111I 699 GACGCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoII 152 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AfaI 1095 GTAC GT^AC
1107 GTAC
AlwI 153 GATCC GGATC(4/5)
1299 GGATC
Asp700I 402 GAAGCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1383 GAACGGCTTC
BglI 683 GCCAGCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1369 GCCATCCAGGC
BsaWI 398 ACCGGA [AT]^CCGG[AT]
1081 TCCGGA
BsgI 316 CTGCAC GTGCAG(16/14)
895 CTGCAC
BsmAI 1060 GAGAC GTCTC(1/5)
1235 GAGAC
BsrBI 187 CCGCTC CCGCTC(-3/-3)
649 GAGCGG
BssSI 319 CACGAG CACGAG(-5/-1)
937 CTCGTG
BstXI 614 CCAGCCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1224 CCAGGAGTTTGG
HaeII 841 GGCGCC [GA]GCGC^[CT]
1357 AGCGCC
MaeIII 824 GTCAC ^GT[ACGT]AC
1199 GTAAC
PleI 798 GAGTC GAGTC(4/5)
1085 GAGTC
PstI 49 CTGCAG CTGCA^G
1165 CTGCAG
PvuII 685 CAGCTG CAG^CTG
1420 CAGCTG
RsaI 1095 GTAC GT^AC
1107 GTAC
SmaI 94 CCCGGG CCC^GGG
1440 CCCGGG
TaqI 194 TCGA T^CGA
1302 TCGA
TthHB8I 194 TCGA T^CGA
1302 TCGA
XcmI 573 CCACGCCTTGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1286 CCAAGGTGTTTATGG
XmaCI 94 CCCGGG C^CCGGG
1440 CCCGGG
XmaI 94 CCCGGG C^CCGGG
1440 CCCGGG
XmnI 402 GAAGCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1383 GAACGGCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 73 enzymes) #
#########################################
AccII AciI AcyI AluI AspHI
AvaI AvaII BanI BanII BbvI
BcnI BmyI BsaHI BsaJI BseRI
BsiHKAI BsiYI BslI BsmFI BsoBI
Bsp1286I BssKI BstF5I BstNI BstUI
Cac8I CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI EarI EcoO109I
EcoRII FauI Fnu4HI FokI HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI Ksp632I
KspI MboI MboII MnlI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV PpuMI
SacII Sau3AI Sau96I ScrFI SfaNI
SfcI TseI TspRI