Restriction map for
pFN21AE2288
##################################
# NO CUT ENZYMES ( 158 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AflII AflIII
AgeI AhdI Alw44I AlwNI AocI
Aor51HI ApaI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AvaI AviII AvrII BamHI
BanI BbeI BbvCI BcgI BciVI
BfaI BfrI BglI BglII BlnI
BsaBI BsaHI BsaI BseAI BseRI
BsiEI BsiWI BsmBI BsmI BsoBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrBI BsrDI BsrFI BsrGI
BssHII Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstXI BstZ17I Bsu36I
Cac8I Cfr10I ClaI CpoI DraI
EagI Eam1105I EarI EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoRI
EcoRV EcoT22I FauI FseI FspI
Hin1I HincII HindII HpaI HphI
KasI KpnI Ksp632I KspI MaeI
MamI MfeI MluI MroI MseI
MunI MvnI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PmeI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI StuI SwaI
Tru9I Tth111I Van91I XbaI XhoI
XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AciI 687 CCGC CCGC(-3/-1)
AspHI 34 GTGCTC G[AT]GC[AT]^C
BanII 597 GAGCCC G[GA]GC[CT]^C
BbrPI 432 CACGTG CAC^GTG
BbsI 218 GAAGAC GAAGAC(2/6)
BbvI 437 GCAGC GCAGC(8/12)
BclI 182 TGATCA T^GATCA
BcnI 522 CCCGG CC^[GC]GG
BlpI 440 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 440 GCTCAGC GC^T[ACGT]AGC
BpuAI 218 GAAGAC GAAGAC(2/6)
BsaWI 326 TCCGGT [AT]^CCGG[AT]
BsgI 435 GTGCAG GTGCAG(16/14)
BsiHKAI 34 GTGCTC G[AT]GC[AT]^C
BsmFI 71 GGGAC GGGAC(10/14)
BssSI 712 CTCGTG CACGAG(-5/-1)
CelII 440 GCTCAGC GC^T[ACGT]AGC
CfoI 288 GCGC GCG^C
DraII 540 GGGTCCT [GA]G^G[ACGT]CC[CT]
DraIII 845 CACCAAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 550 GACCTCAATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 695 CCGTGG C^C[GA][CT]GG
EcoO109I 540 GGGTCCT [GA]G^G[ACGT]CC[CT]
FbaI 182 TGATCA T^GATCA
Fnu4HI 437 GCAGC GC^[ACGT]GC
HaeII 287 GGCGCT [GA]GCGC^[CT]
HhaI 288 GCGC GCG^C
HinP1I 288 GCGC G^CGC
HindIII 161 AAGCTT A^AGCTT
ItaI 437 GCAGC GC^[ACGT]GC
MboII 218 GAAGA GAAGA(8/7)
MspA1I 175 CAGCTG C[AC]G^C[GT]G
NciI 522 CCCGG CC^[GC]GG
PmaCI 432 CACGTG CAC^GTG
PmlI 432 CACGTG CAC^GTG
PpuMI 540 GGGTCCT [GA]G^G[AT]CC[CT]
PstI 386 CTGCAG CTGCA^G
PvuII 175 CAGCTG CAG^CTG
SfaNI 408 GATGC GCATC(5/9)
SspI 421 AATATT AAT^ATT
TaqI 103 TCGA T^CGA
TseI 437 GCAGC G^C[AT]GC
Tsp509I 742 AATT ^AATT
TthHB8I 103 TCGA T^CGA
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
BalI 473 TGGCCA TGG^CCA
725 TGGCCA
BpmI 343 CTGGAG CTGGAG(16/14)
801 CTGGAG
BsaAI 198 CACGTA [CT]AC^GT[GA]
432 CACGTG
BsmAI 238 GAGAC GTCTC(1/5)
559 GTCTC
BstYI 127 GGATCT [GA]^GATC[CT]
566 GGATCT
HapII 327 CCGG C^CGG
523 CCGG
HgaI 271 GACGC GACGC(5/10)
672 GACGC
HpaII 327 CCGG C^CGG
523 CCGG
MaeII 199 ACGT A^CGT
433 ACGT
MflI 127 GGATCT [GA]^GATC[CT]
566 GGATCT
MluNI 473 TGGCCA TGG^CCA
725 TGGCCA
MscI 473 TGGCCA TGG^CCA
725 TGGCCA
MslI 505 CACCAAAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
690 CATGGCCGTG
MspI 327 CCGG C^CGG
523 CCGG
PleI 649 GACTC GAGTC(4/5)
105 GAGTC
XcmI 527 CCAACTTCTCTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
620 CCAGTAATGCCTTGG
XhoII 127 GGATCT [GA]^GATC[CT]
566 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 39 enzymes) #
#########################################
AfaI AluI AlwI AvaII BmyI
BsaJI BsiYI BslI Bsp1286I BsrI
BssKI BstF5I BstNI Cfr13I DdeI
DpnII EaeI EcoRII EcoT14I FokI
HaeIII HinfI MaeIII MboI MnlI
MvaI MwoI NdeII NlaIII NlaIV
RsaI Sau3AI Sau96I ScrFI SfcI
StyI TfiI Tsp45I TspRI