Restriction map for
pFN21AE5715
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# NO CUT ENZYMES ( 94 enzymes) #
##################################
AatII Acc65I AccI AclI AcyI
Alw44I AocI Aor51HI ApaLI AscI
Asp700I Asp718I AvrII BamHI BbeI
BbrPI BciVI BglI BglII BlnI
BsaBI BsaHI BsgI BsiEI BsiWI
BspDI BspLU11I BspMI BsrBI BsrDI
BssHII Bst1107I BstEII BstPI BstXI
BstZ17I Bsu36I ClaI CpoI DraI
DraIII DrdI EagI EclXI Eco47III
Eco52I Eco81I EcoO65I EcoRI EcoRV
EcoT22I FseI Hin1I KasI KpnI
KspI MamI MfeI MunI NaeI
NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI PacI PflMI
PmaCI PmeI PmlI Psp1406I PvuI
RsrII SacII SalI SbfI SfiI
SfoI SgrAI SmaI SnaBI Sse8387I
SspI StuI SwaI TfiI Van91I
XbaI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 64 enzymes) #
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AflIII 161 ACGCGT A^C[GA][CT]GT
AgeI 1876 ACCGGT A^CCGGT
AhdI 1446 GACAGTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AseI 328 ATTAAT AT^TAAT
AsnI 328 ATTAAT AT^TAAT
AspEI 1446 GACAGTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1277 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 1774 CTCGAG C^[CT]CG[GA]G
AviII 1499 TGCGCA TGC^GCA
BalI 1073 TGGCCA TGG^CCA
BanI 1303 GGTGCC G^G[CT][GA]CC
BbsI 598 GAAGAC GAAGAC(2/6)
BbvCI 1543 CCTCAGC CCTCAGC(-5/-2)
BcgI 296 GCAACATATTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 137 TGATCA T^GATCA
BcnI 96 CCCGG CC^[GC]GG
BlpI 1102 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1102 GCTCAGC GC^T[ACGT]AGC
BpuAI 598 GAAGAC GAAGAC(2/6)
BsaAI 40 TACGTG [CT]AC^GT[GA]
BsmBI 925 CGTCTC CGTCTC(1/5)
BsmI 479 GAATGC GAATGC(1/-1)
BsoBI 1774 CTCGAG C^[CT]CG[GA]G
BspHI 1208 TCATGA T^CATGA
BsrFI 1876 ACCGGT [GA]^CCGG[CT]
BssSI 1403 CACGAG CACGAG(-5/-1)
BstAPI 1465 GCAGCTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 278 TTCGAA TT^CGAA
CelII 1102 GCTCAGC GC^T[ACGT]AGC
Cfr10I 1876 ACCGGT [GA]^CCGG[CT]
DsaI 1510 CCACGG C^C[GA][CT]GG
EaeI 1073 TGGCCA [CT]^GGCC[GA]
Eam1105I 1446 GACAGTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 1114 GAAGAG CTCTTC(1/4)
EcoNI 1192 CCTCCTTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 410 CCAAGG C^C[AT][AT]GG
FbaI 137 TGATCA T^GATCA
FspI 1499 TGCGCA TGC^GCA
HgaI 190 GACGC GACGC(5/10)
HindIII 708 AAGCTT A^AGCTT
HpaI 1864 GTTAAC GTT^AAC
Ksp632I 1114 GAAGAG CTCTTC(1/4)
MluI 161 ACGCGT A^CGCGT
MluNI 1073 TGGCCA TGG^CCA
MscI 1073 TGGCCA TGG^CCA
NciI 96 CCCGG CC^[GC]GG
NspV 278 TTCGAA TT^CGAA
PaeR7I 1774 CTCGAG C^TCGAG
PflFI 1277 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 1876 ACCGGT A^CCGGT
PpuMI 47 GGGACCT [GA]G^G[AT]CC[CT]
PshAI 1212 GACAGCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 328 ATTAAT AT^TAAT
RcaI 1208 TCATGA T^CATGA
SapI 1114 GAAGAGC GCTCTTC(1/4)
ScaI 1372 AGTACT AGT^ACT
SexAI 872 ACCAGGT A^CC[AT]GGT
SfuI 278 TTCGAA TT^CGAA
SpeI 961 ACTAGT A^CTAGT
SphI 198 GCATGC GCATG^C
StyI 410 CCAAGG C^C[AT][AT]GG
Tth111I 1277 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 1279 CCAAGTCCTCGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 1774 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AccII 162 CGCG CG^CG
192 CGCG
AccIII 119 TCCGGA T^CCGGA
929 TCCGGA
AlwNI 353 CAGTTTCTG CAG[ACGT][ACGT][ACGT]^CTG
1466 CAGCTGCTG
ApaI 103 GGGCCC GGGCC^C
643 GGGCCC
AvaII 48 GGACC G^G[AT]CC
1453 GGTCC
BpmI 1626 CTCCAG CTGGAG(16/14)
1781 CTCCAG
BsaI 1380 GAGACC GGTCTC(1/5)
257 GGTCTC
BseAI 119 TCCGGA T^CCGGA
929 TCCGGA
BsiYI 1192 CCTCCTTCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1794 CCGTTCTAAGG
BslI 1192 CCTCCTTCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1794 CCGTTCTAAGG
Bsp1407I 426 TGTACA T^GTACA
1599 TGTACA
BspEI 119 TCCGGA T^CCGGA
929 TCCGGA
BsrGI 426 TGTACA T^GTACA
1599 TGTACA
BstUI 162 CGCG CG^CG
192 CGCG
BstYI 122 GGATCT [GA]^GATC[CT]
624 GGATCT
DraII 47 GGGACCT [GA]G^G[ACGT]CC[CT]
102 AGGGCCC
EcoO109I 47 GGGACCT [GA]G^G[ACGT]CC[CT]
102 AGGGCCC
HaeII 614 AGCGCC [GA]GCGC^[CT]
1118 AGCGCC
HincII 1413 GTCAAC GT[CT]^[GA]AC
1864 GTTAAC
HindII 1413 GTCAAC GT[CT]^[GA]AC
1864 GTTAAC
MflI 122 GGATCT [GA]^GATC[CT]
624 GGATCT
MroI 119 TCCGGA T^CCGGA
929 TCCGGA
MvnI 162 CGCG CG^CG
192 CGCG
NspI 198 GCATGC [GA]CATG^[CT]
1733 GCATGT
SacI 763 GAGCTC GAGCT^C
794 GAGCTC
SspBI 426 TGTACA T^GTACA
1599 TGTACA
Tsp45I 64 GTGAC ^GT[GC]AC
1788 GTCAC
XhoII 122 GGATCT [GA]^GATC[CT]
624 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 72 enzymes) #
#########################################
AciI AcsI AfaI AflII AluI
AlwI ApoI AspHI BanII BbvI
BfaI BfrI BmyI BsaJI BsaWI
BseRI BsiHKAI BsmAI BsmFI Bsp1286I
BsrI BssKI BstF5I BstNI Cac8I
CfoI Cfr13I DdeI DpnII EcoRII
FauI Fnu4HI FokI HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI MaeI MaeII MaeIII MboI
MboII MnlI MseI MslI MspA1I
MspI MvaI MwoI NdeII NlaIII
NlaIV PleI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI TaqI Tru9I TseI Tsp509I
TspRI TthHB8I