Restriction map for
pFN21AE5612
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# NO CUT ENZYMES ( 154 enzymes) #
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AatII Acc65I AccII AclI AcyI
AflII AflIII AgeI AhdI Alw44I
AlwNI AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp718I AspEI
AspI AvaI AvaII AvrII BalI
BamHI BanI BanII BbeI BbrPI
BbvCI BfrI BglI BglII BlnI
BlpI Bpu1102I BsaBI BsaHI BseRI
BsgI BsiEI BsiWI BsiYI BslI
BsmBI BsmFI BsoBI BspDI BspHI
BspLU11I BspMI BsrBI BsrDI BsrFI
BssHII BssSI BstAPI BstBI BstEII
BstPI BstUI BstXI BstYI Bsu36I
CelII Cfr10I ClaI CpoI DraI
DraII DraIII DrdI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO109I EcoO65I EcoRV EcoT22I FseI
HaeII HgaI Hin1I HincII HindII
HpaI KasI KpnI KspI MamI
MfeI MflI MluI MluNI MscI
MslI MunI MvnI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PstI PvuI RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
StuI SwaI Tth111I Van91I XbaI
XhoI XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 55 enzymes) #
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AccIII 663 TCCGGA T^CCGGA
AciI 912 CCGC CCGC(-3/-1)
AlwI 1503 GGATC GGATC(4/5)
AspHI 116 GAGCAC G[AT]GC[AT]^C
AviII 833 TGCGCA TGC^GCA
BbsI 1270 GAAGAC GAAGAC(2/6)
BcgI 978 CGAAACTGTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 58 GTATCC GTATCC(6/5)
BclI 927 TGATCA T^GATCA
BmyI 116 GAGCAC G[AGT]GC[ACT]^C
BpmI 1276 CTGGAG CTGGAG(16/14)
BpuAI 1270 GAAGAC GAAGAC(2/6)
BsaAI 1250 TACGTG [CT]AC^GT[GA]
BsaI 450 GAGACC GGTCTC(1/5)
BsaJI 1415 CCATGG C^C[ACGT][ACGT]GG
BseAI 663 TCCGGA T^CCGGA
BsiHKAI 116 GAGCAC G[AT]GC[AT]^C
BsmI 159 GAATGC GAATGC(1/-1)
Bsp1286I 116 GAGCAC G[AGT]GC[ACT]^C
Bsp1407I 528 TGTACA T^GTACA
BspEI 663 TCCGGA T^CCGGA
BsrGI 528 TGTACA T^GTACA
Bst1107I 966 GTATAC GTA^TAC
BstF5I 782 CATCC GGATG(2/0)
BstZ17I 966 GTATAC GTA^TAC
Cac8I 1052 GCTTGC GC[ACGT]^[ACGT]GC
CfoI 834 GCGC GCG^C
Cfr13I 152 GGGCC G^G[ACGT]CC
DsaI 1415 CCATGG C^C[GA][CT]GG
EaeI 631 CGGCCA [CT]^GGCC[GA]
EarI 214 CTCTTC CTCTTC(1/4)
EcoRI 1176 GAATTC G^AATTC
EcoT14I 1415 CCATGG C^C[AT][AT]GG
FauI 911 CCCGC CCCGC(4/6)
FbaI 927 TGATCA T^GATCA
FokI 782 CATCC GGATG(9/13)
FspI 833 TGCGCA TGC^GCA
HhaI 834 GCGC GCG^C
HinP1I 834 GCGC G^CGC
HindIII 36 AAGCTT A^AGCTT
Ksp632I 214 CTCTTC CTCTTC(1/4)
MroI 663 TCCGGA T^CCGGA
MseI 44 TTAA T^TAA
NcoI 1415 CCATGG C^CATGG
NlaIV 1018 GGAACC GG[ACGT]^[ACGT]CC
PleI 102 GACTC GAGTC(4/5)
Sau96I 152 GGGCC G^G[ACGT]CC
SfcI 812 CTGTAG C^T[GA][CT]AG
SspBI 528 TGTACA T^GTACA
SspI 267 AATATT AAT^ATT
StyI 1415 CCATGG C^C[AT][AT]GG
TaqI 840 TCGA T^CGA
Tru9I 44 TTAA T^TAA
TthHB8I 840 TCGA T^CGA
XcmI 634 CCAAATCACTTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 12 enzymes) #
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AccI 409 GTAGAC GT^[AC][GT]AC
966 GTATAC
AfaI 529 GTAC GT^AC
1249 GTAC
Asp700I 508 GAATTGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
588 GAAGGATTTC
BcnI 251 CCGGG CC^[GC]GG
694 CCCGG
BsaWI 663 TCCGGA [AT]^CCGG[AT]
894 TCCGGT
MaeII 1103 ACGT A^CGT
1251 ACGT
MspA1I 747 CAGCTG C[AC]G^C[GT]G
1305 CAGCTG
NciI 251 CCGGG CC^[GC]GG
694 CCCGG
PvuII 747 CAGCTG CAG^CTG
1305 CAGCTG
RsaI 529 GTAC GT^AC
1249 GTAC
Tsp45I 1105 GTCAC ^GT[GC]AC
1184 GTGAC
XmnI 508 GAATTGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
588 GAAGGATTTC
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# 3 OR MORE CUT ENZYMES ( 37 enzymes) #
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AcsI AluI ApoI BbvI BfaI
BsmAI BsrI BssKI BstNI DdeI
DpnII EcoRII Fnu4HI HaeIII HapII
HinfI HpaII HphI ItaI MaeI
MaeIII MboI MboII MnlI MspI
MvaI MwoI NdeII NlaIII Sau3AI
ScrFI SfaNI SmlI TfiI TseI
Tsp509I TspRI