Restriction map for
pFN21AE6339
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# NO CUT ENZYMES ( 151 enzymes) #
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AatII Acc65I AccIII AclI AcyI
AflII AflIII AgeI Alw44I AlwI
AlwNI AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspHI AspI AvaI AviII AvrII
BalI BamHI BanI BanII BbeI
BbrPI BbsI BbvCI BcgI BclI
BfrI BglI BglII BlnI BlpI
BmyI Bpu1102I BpuAI BsaBI BsaHI
BsaWI BseAI BseRI BsgI BsiEI
BsiHKAI BsiWI BsmBI BsmI BsoBI
Bsp1286I BspDI BspEI BspLU11I BspMI
BsrBI BssHII BssSI Bst1107I BstAPI
BstXI BstYI BstZ17I Bsu36I CelII
ClaI CpoI DpnII DraII DraIII
DrdI EagI EarI EclXI Eco47III
Eco52I Eco81I EcoO109I EcoRI EcoRV
EcoT22I FbaI FseI FspI HgaI
Hin1I HindIII KasI KpnI Ksp632I
KspI MamI MboI MfeI MflI
MluI MluNI MroI MscI MunI
NarI NdeII NheI NotI NruI
NsiI PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI RsrII
SacI SacII SalI SapI Sau3AI
SbfI ScaI SexAI SfiI SfoI
SgrAI SmaI SmlI SpeI SphI
Sse8387I SspI Tth111I Van91I XbaI
XcmI XhoI XhoII XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AccI 1021 GTAGAC GT^[AC][GT]AC
AccII 15 CGCG CG^CG
AcsI 753 AAATTC [GA]^AATT[CT]
AhdI 249 GACCAACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApoI 753 AAATTC [GA]^AATT[CT]
AspEI 249 GACCAACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaII 231 GGACC G^G[AT]CC
BcnI 171 CCCGG CC^[GC]GG
BpmI 205 CTCCAG CTGGAG(16/14)
BsaAI 928 TACGTA [CT]AC^GT[GA]
BsaI 1088 GGTCTC GGTCTC(1/5)
BsmAI 1089 GTCTC GTCTC(1/5)
BsrFI 4 GCCGGC [GA]^CCGG[CT]
BstBI 962 TTCGAA TT^CGAA
BstEII 718 GGTCACC G^GT[ACGT]ACC
BstPI 718 GGTCACC G^GT[ACGT]ACC
BstUI 15 CGCG CG^CG
Cfr10I 4 GCCGGC [GA]^CCGG[CT]
Cfr13I 231 GGACC G^G[ACGT]CC
DsaI 789 CCATGG C^C[GA][CT]GG
Eam1105I 249 GACCAACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 760 CCTACCAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 718 GGTCACC G^GT[ACGT]ACC
EcoT14I 789 CCATGG C^C[AT][AT]GG
FauI 103 GCGGG CCCGC(4/6)
MvnI 15 CGCG CG^CG
MwoI 808 GCAGTGAGAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NaeI 4 GCCGGC GCC^GGC
NciI 171 CCCGG CC^[GC]GG
NcoI 789 CCATGG C^CATGG
NgoMIV 4 GCCGGC G^CCGGC
NlaIV 160 GGTTCC GG[ACGT]^[ACGT]CC
NspV 962 TTCGAA TT^CGAA
PleI 277 GACTC GAGTC(4/5)
PstI 291 CTGCAG CTGCA^G
Sau96I 231 GGACC G^G[ACGT]CC
SfcI 291 CTGCAG C^T[GA][CT]AG
SfuI 962 TTCGAA TT^CGAA
SnaBI 928 TACGTA TAC^GTA
StuI 178 AGGCCT AGG^CCT
StyI 789 CCATGG C^C[AT][AT]GG
SwaI 1076 ATTTAAAT ATTT^AAAT
TspRI 809 CAGTG CA[GC]TG[ACGT][ACGT]^
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# TWO CUT ENZYMES ( 25 enzymes) #
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BciVI 394 GGATAC GTATCC(6/5)
975 GGATAC
BsaJI 170 CCCCGG C^C[ACGT][ACGT]GG
789 CCATGG
BsmFI 107 GGGAC GGGAC(10/14)
1283 GTCCC
Bsp1407I 825 TGTACA T^GTACA
922 TGTACA
BspHI 371 TCATGA T^CATGA
419 TCATGA
BsrDI 345 CATTGC GCAATG(2/0)
399 CATTGC
BsrGI 825 TGTACA T^GTACA
922 TGTACA
BsrI 228 ACTGG ACTGG(1/-1)
711 CCAGT
BstF5I 48 GGATG GGATG(2/0)
96 GGATG
Cac8I 4 GCCGGC GC[ACGT]^[ACGT]GC
474 GCTTGC
DraI 66 TTTAAA TTT^AAA
1077 TTTAAA
EaeI 2 TGGCCG [CT]^GGCC[GA]
149 TGGCCG
FokI 48 GGATG GGATG(9/13)
96 GGATG
HapII 5 CCGG C^CGG
172 CCGG
HpaI 184 GTTAAC GTT^AAC
441 GTTAAC
HpaII 5 CCGG C^CGG
172 CCGG
MaeII 783 ACGT A^CGT
929 ACGT
MboII 1054 GAAGA GAAGA(8/7)
1072 GAAGA
MslI 1012 CATTTGAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1148 CATCTAAATG
MspI 5 CCGG C^CGG
172 CCGG
NspI 828 ACATGC [GA]CATG^[CT]
1205 GCATGT
PvuII 259 CAGCTG CAG^CTG
665 CAGCTG
RcaI 371 TCATGA T^CATGA
419 TCATGA
SfaNI 52 GATGC GCATC(5/9)
427 GATGC
SspBI 825 TGTACA T^GTACA
922 TGTACA
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# 3 OR MORE CUT ENZYMES ( 39 enzymes) #
#########################################
AciI AfaI AluI BbvI BfaI
BsiYI BslI BssKI BstNI CfoI
DdeI EcoRII Fnu4HI HaeII HaeIII
HhaI HinP1I HincII HindII HinfI
HphI ItaI MaeI MaeIII MnlI
MseI MspA1I MvaI NdeI NlaIII
RsaI ScrFI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TthHB8I