Restriction map for
pFN21AE1464
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# NO CUT ENZYMES ( 196 enzymes) #
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AatII Acc65I AccII AccIII AciI
AclI AcsI AcyI AflII AflIII
AgeI AhdI Alw44I AlwNI AocI
Aor51HI ApaI ApaLI ApoI AscI
AseI AsnI Asp718I AspEI AspHI
AspI AvaI AvaII AviII AvrII
BalI BamHI BanII BbeI BbrPI
BbvCI BbvI BcgI BciVI BcnI
BfrI BglI BglII BlnI BlpI
Bpu1102I BsaAI BsaBI BsaHI BsaI
BsaWI BseAI BseRI BsiEI BsiHKAI
BsiWI BsmAI BsmBI BsmI BsoBI
BspDI BspEI BspLU11I BspMI BsrBI
BsrDI BsrFI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstUI BstXI
BstYI BstZ17I Bsu36I CelII CfoI
Cfr10I Cfr13I ClaI CpoI DraI
DraII DrdI DsaI EaeI EagI
Eam1105I EarI EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoRI
EcoRV EcoT14I EcoT22I FauI Fnu4HI
FseI FspI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HincII
HindII HindIII HpaI HpaII HphI
ItaI KasI KpnI Ksp632I KspI
MaeII MamI MfeI MflI MluI
MluNI MroI MscI MspA1I MspI
MunI MvnI NaeI NarI NciI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PleI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PstI PvuI PvuII RsrII SacI
SacII SalI SapI Sau96I SbfI
ScaI SexAI SfcI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I StuI StyI
SwaI TaqI TseI Tth111I TthHB8I
XbaI XcmI XhoI XhoII XmaCI
XmaI
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# ONE CUT ENZYMES ( 36 enzymes) #
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AccI 457 GTAGAC GT^[AC][GT]AC
AfaI 126 GTAC GT^AC
AluI 130 AGCT AG^CT
Asp700I 493 GAAAACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BanI 17 GGTGCC G^G[CT][GA]CC
BbsI 172 GAAGAC GAAGAC(2/6)
BclI 339 TGATCA T^GATCA
BfaI 43 CTAG C^TAG
BmyI 18 GTGCCC G[AGT]GC[ACT]^C
BpmI 135 CTCCAG CTGGAG(16/14)
BpuAI 172 GAAGAC GAAGAC(2/6)
BsgI 296 CTGCAC GTGCAG(16/14)
BsmFI 36 GGGAC GGGAC(10/14)
Bsp1286I 18 GTGCCC G[AGT]GC[ACT]^C
Bsp1407I 125 TGTACA T^GTACA
BspHI 501 TCATGA T^CATGA
BsrGI 125 TGTACA T^GTACA
BsrI 137 CCAGT ACTGG(1/-1)
BstAPI 424 GCAATAGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Cac8I 433 GCCAGC GC[ACGT]^[ACGT]GC
DraIII 94 CACTATGTG CAC[ACGT][ACGT][ACGT]^GTG
FbaI 339 TGATCA T^GATCA
HinfI 8 GAATC G^A[ACGT]TC
MaeI 43 CTAG C^TAG
MseI 350 TTAA T^TAA
MslI 317 CACAGATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NlaIV 17 GGTGCC GG[ACGT]^[ACGT]CC
NspI 420 ACATGC [GA]CATG^[CT]
RcaI 501 TCATGA T^CATGA
RsaI 126 GTAC GT^AC
SspBI 125 TGTACA T^GTACA
SspI 85 AATATT AAT^ATT
TfiI 8 GAATC G^A[AT]TC
Tru9I 350 TTAA T^TAA
Tsp45I 140 GTGAC ^GT[GC]AC
XmnI 493 GAAAACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 13 enzymes) #
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AlwI 508 GATCC GGATC(4/5)
531 GATCC
BsaJI 149 CCAGGG C^C[ACGT][ACGT]GG
370 CCCAGG
BstF5I 222 GGATG GGATG(2/0)
402 GGATG
FokI 222 GGATG GGATG(9/13)
402 GGATG
MaeIII 140 GTGAC ^GT[ACGT]AC
191 GTAAC
MboII 172 GAAGA GAAGA(8/7)
80 TCTTC
MwoI 360 GCGTAGAAAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
424 GCAATAGATGC
NlaIII 421 CATG CATG^
502 CATG
PflMI 93 CCACTATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
181 CCAATTTATGG
SfaNI 276 GATGC GCATC(5/9)
430 GATGC
Tsp509I 183 AATT ^AATT
229 AATT
TspRI 74 CACTG CA[GC]TG[ACGT][ACGT]^
138 CAGTG
Van91I 93 CCACTATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
181 CCAATTTATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 13 enzymes) #
#########################################
BsiYI BslI BssKI BstNI DdeI
DpnII EcoRII MboI MnlI MvaI
NdeII Sau3AI ScrFI