Restriction map for
pFN21AE6440
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# NO CUT ENZYMES ( 128 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AflII AflIII AgeI Alw44I
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp718I AspHI AvaI
AvrII BamHI BbrPI BcgI BciVI
BclI BfrI BglI BlnI BsaAI
BsaWI BseAI BseRI BsiEI BsiHKAI
BsiWI BsmBI BsoBI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrBI BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstZ17I Bsu36I Cfr10I ClaI CpoI
DraI DraII DraIII DrdI EagI
EclXI Eco47III Eco52I Eco81I EcoO109I
EcoO65I EcoRI EcoT22I FbaI FseI
HincII HindII HpaI HphI KpnI
KspI MfeI MluI MroI MunI
MvnI NaeI NcoI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfiI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SwaI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AcyI 30 GGCGCC G[GA]^CG[CT]C
AhdI 422 GACAAATTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 422 GACAAATTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1141 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaII 183 GGTCC G^G[AT]CC
AviII 1332 TGCGCA TGC^GCA
BanI 30 GGCGCC G^G[CT][GA]CC
BbeI 30 GGCGCC GGCGC^C
BbsI 1389 GTCTTC GAAGAC(2/6)
BbvCI 62 CCTCAGC CCTCAGC(-5/-2)
BcnI 486 CCCGG CC^[GC]GG
BglII 500 AGATCT A^GATCT
BlpI 291 GCTTAGC GC^T[ACGT]AGC
Bpu1102I 291 GCTTAGC GC^T[ACGT]AGC
BpuAI 1389 GTCTTC GAAGAC(2/6)
BsaBI 667 GATGTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 30 GGCGCC G[GA]^CG[CT]C
BsaI 1709 GAGACC GGTCTC(1/5)
BsmI 306 GAATGC GAATGC(1/-1)
BstYI 500 AGATCT [GA]^GATC[CT]
CelII 291 GCTTAGC GC^T[ACGT]AGC
Eam1105I 422 GACAAATTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 1662 CCTGGGAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 1292 GATATC GAT^ATC
FspI 1332 TGCGCA TGC^GCA
HaeII 30 GGCGCC [GA]GCGC^[CT]
HapII 487 CCGG C^CGG
HgaI 829 GCGTC GACGC(5/10)
Hin1I 30 GGCGCC G[GA]^CG[CT]C
HindIII 1056 AAGCTT A^AGCTT
HpaII 487 CCGG C^CGG
KasI 30 GGCGCC G^GCGCC
MamI 667 GATGTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 500 AGATCT [GA]^GATC[CT]
MslI 115 CACACCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 788 CAGCTG C[AC]G^C[GT]G
MspI 487 CCGG C^CGG
NarI 30 GGCGCC GG^CGCC
NciI 486 CCCGG CC^[GC]GG
NdeI 728 CATATG CA^TATG
PflFI 1141 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PstI 1125 CTGCAG CTGCA^G
PvuII 788 CAGCTG CAG^CTG
SfoI 30 GGCGCC GGC^GCC
SmlI 164 CTCAAG C^T[CT][GA]AG
StuI 188 AGGCCT AGG^CCT
Tth111I 1141 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 1215 CCACCTGCCCAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 500 AGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 22 enzymes) #
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AfaI 639 GTAC GT^AC
1341 GTAC
AlwNI 1192 CAGACTCTG CAG[ACGT][ACGT][ACGT]^CTG
1368 CAGCCCCTG
BalI 354 TGGCCA TGG^CCA
494 TGGCCA
BanII 794 GGGCTC G[GA]GC[CT]^C
1570 GAGCCC
BfaI 458 CTAG C^TAG
1064 CTAG
BsgI 207 GTGCAG GTGCAG(16/14)
1639 GTGCAG
BstXI 897 CCAACTCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1592 CCAACGAGGTGG
DsaI 1377 CCACGG C^C[GA][CT]GG
1574 CCGTGG
EcoT14I 386 CCAAGG C^C[AT][AT]GG
1713 CCAAGG
MaeI 458 CTAG C^TAG
1064 CTAG
MaeII 1184 ACGT A^CGT
1402 ACGT
MaeIII 323 GTCAC ^GT[ACGT]AC
1139 GTGAC
MluNI 354 TGGCCA TGG^CCA
494 TGGCCA
MscI 354 TGGCCA TGG^CCA
494 TGGCCA
PflMI 712 CCAAAAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1656 CCAAATCCTGG
RsaI 639 GTAC GT^AC
1341 GTAC
SfaNI 1024 GCATC GCATC(5/9)
1203 GCATC
SspI 452 AATATT AAT^ATT
1006 AATATT
StyI 386 CCAAGG C^C[AT][AT]GG
1713 CCAAGG
Tsp45I 323 GTCAC ^GT[GC]AC
1139 GTGAC
Van91I 712 CCAAAAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1656 CCAAATCCTGG
XbaI 457 TCTAGA T^CTAGA
1063 TCTAGA
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# 3 OR MORE CUT ENZYMES ( 59 enzymes) #
#########################################
AciI AcsI AluI AlwI ApoI
Asp700I BbvI BmyI BpmI BsaJI
BsiYI BslI BsmAI BsmFI Bsp1286I
BspMI BsrI BssKI BstF5I BstNI
Cac8I CfoI Cfr13I DdeI DpnII
EaeI EarI EcoRII FauI Fnu4HI
FokI HaeIII HhaI HinP1I HinfI
ItaI Ksp632I MboI MboII MnlI
MseI MvaI MwoI NdeII NlaIII
NlaIV PleI Sau3AI Sau96I ScrFI
SfcI TaqI TfiI Tru9I TseI
Tsp509I TspRI TthHB8I XmnI