Restriction map for
pFN21AE3631
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# NO CUT ENZYMES ( 165 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AgeI AhdI
Alw44I AlwNI AocI Aor51HI ApaI
ApaLI AscI Asp700I Asp718I AspEI
AspI AvaI AviII AvrII BalI
BamHI BanI BanII BbeI BbrPI
BcgI BciVI BclI BfrI BglI
BglII BlnI BsaAI BsaHI BsaI
BsaWI BseAI BseRI BsgI BsiEI
BsiWI BsmBI BsmFI BsmI BsoBI
BspDI BspEI BspHI BspMI BsrBI
BsrFI BssHII BssSI Bst1107I BstAPI
BstBI BstNI BstUI BstYI BstZ17I
Bsu36I CfoI Cfr10I ClaI CpoI
DraI DraII DrdI DsaI EaeI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoRI EcoRII
EcoRV EcoT14I EcoT22I FauI FbaI
FseI FspI HaeII HaeIII HgaI
HhaI Hin1I HinP1I HincII HindII
HpaI KasI KpnI KspI MaeII
MfeI MflI MluI MluNI MroI
MscI MunI MvaI MvnI NaeI
NarI NcoI NgoMIV NheI NlaIV
NotI NruI NsiI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PpuMI Psp1406I PvuI
RcaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI Sse8387I StuI StyI SwaI
TaqI Tth111I TthHB8I Van91I XbaI
XhoI XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 57 enzymes) #
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AcsI 883 AAATTT [GA]^AATT[CT]
AflIII 692 ACATGT A^C[GA][CT]GT
AlwI 714 GGATC GGATC(4/5)
ApoI 883 AAATTT [GA]^AATT[CT]
AseI 13 ATTAAT AT^TAAT
AsnI 13 ATTAAT AT^TAAT
AspHI 688 GAGCAC G[AT]GC[AT]^C
AvaII 63 GGTCC G^G[AT]CC
BbsI 700 GAAGAC GAAGAC(2/6)
BbvCI 799 GCTGAGG CCTCAGC(-5/-2)
BcnI 60 CCCGG CC^[GC]GG
BlpI 416 GCTCAGC GC^T[ACGT]AGC
BmyI 688 GAGCAC G[AGT]GC[ACT]^C
BpmI 125 CTGGAG CTGGAG(16/14)
Bpu1102I 416 GCTCAGC GC^T[ACGT]AGC
BpuAI 700 GAAGAC GAAGAC(2/6)
BsaBI 325 GATACATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaJI 59 CCCCGG C^C[ACGT][ACGT]GG
BsiHKAI 688 GAGCAC G[AT]GC[AT]^C
BsiYI 54 CCTGACCCCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 54 CCTGACCCCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI 823 GAGAC GTCTC(1/5)
Bsp1286I 688 GAGCAC G[AGT]GC[ACT]^C
Bsp1407I 888 TGTACA T^GTACA
BspLU11I 692 ACATGT A^CATGT
BsrDI 159 GCAATG GCAATG(2/0)
BsrGI 888 TGTACA T^GTACA
BssKI 60 CCCGG ^CC[ACGT]GG
BstEII 222 GGTAACC G^GT[ACGT]ACC
BstF5I 316 GGATG GGATG(2/0)
BstPI 222 GGTAACC G^GT[ACGT]ACC
BstXI 637 CCACGATTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 416 GCTCAGC GC^T[ACGT]AGC
Cfr13I 63 GGTCC G^G[ACGT]CC
DraIII 422 CACTATGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoO65I 222 GGTAACC G^GT[ACGT]ACC
FokI 316 GGATG GGATG(9/13)
HapII 61 CCGG C^CGG
HindIII 539 AAGCTT A^AGCTT
HpaII 61 CCGG C^CGG
MamI 325 GATACATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MspA1I 122 CAGCTG C[AC]G^C[GT]G
MspI 61 CCGG C^CGG
NciI 60 CCCGG CC^[GC]GG
NdeI 859 CATATG CA^TATG
PleI 633 GACTC GAGTC(4/5)
PshBI 13 ATTAAT AT^TAAT
PstI 461 CTGCAG CTGCA^G
PvuII 122 CAGCTG CAG^CTG
Sau96I 63 GGTCC G^G[ACGT]CC
ScrFI 60 CCCGG CC^[ACGT]GG
SmlI 67 CTCAAG C^T[CT][GA]AG
SphI 795 GCATGC GCATG^C
SspBI 888 TGTACA T^GTACA
TfiI 73 GAATC G^A[AT]TC
Tsp45I 733 GTGAC ^GT[GC]AC
XcmI 151 CCAACATGGCAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 13 enzymes) #
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AfaI 51 GTAC GT^AC
889 GTAC
BfaI 136 CTAG C^TAG
517 CTAG
BsrI 36 ACTGG ACTGG(1/-1)
381 ACTGG
HinfI 73 GAATC G^A[ACGT]TC
633 GACTC
MaeI 136 CTAG C^TAG
517 CTAG
MaeIII 223 GTAAC ^GT[ACGT]AC
733 GTGAC
MwoI 541 GCTTGTAAAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
666 GCAACGGCAGC
NspI 692 ACATGT [GA]CATG^[CT]
795 GCATGC
PshAI 57 GACCCCGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
703 GACATCAGTC
RsaI 51 GTAC GT^AC
889 GTAC
SfaNI 559 GATGC GCATC(5/9)
574 GATGC
SfcI 167 CTACAG C^T[GA][CT]AG
461 CTGCAG
SspI 266 AATATT AAT^ATT
623 AATATT
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# 3 OR MORE CUT ENZYMES ( 23 enzymes) #
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AciI AluI BbvI Cac8I DdeI
DpnII EarI Fnu4HI HphI ItaI
Ksp632I MboI MboII MnlI MseI
MslI NdeII NlaIII Sau3AI Tru9I
TseI Tsp509I TspRI