Restriction map for
pFN21AE4466
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# NO CUT ENZYMES ( 102 enzymes) #
##################################
Acc65I AccI AclI AcsI AflII
AflIII AgeI AhdI AocI ApoI
AscI AseI AsnI Asp718I AspEI
AviII AvrII BbsI BcgI BciVI
BclI BfaI BfrI BglII BlnI
BpuAI BsiWI Bsp1407I BspDI BspHI
BspLU11I BspMI BsrGI BssHII Bst1107I
BstAPI BstBI BstEII BstPI BstZ17I
Bsu36I ClaI CpoI DraI DrdI
EagI Eam1105I EclXI Eco52I Eco81I
EcoNI EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI FspI HindIII HpaI
KpnI MaeI MfeI MluI MseI
MunI NcoI NdeI NheI NotI
NruI NsiI NspI NspV PacI
PflMI PinAI PmeI PpuMI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SalI ScaI SfuI SmaI SnaBI
SpeI SphI SspBI SspI StuI
SwaI Tru9I Tsp509I Van91I XbaI
XmaCI XmaI
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# ONE CUT ENZYMES ( 60 enzymes) #
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AatII 637 GACGTC GACGT^C
AccIII 818 TCCGGA T^CCGGA
Alw44I 1141 GTGCAC G^TGCAC
AlwNI 536 CAGCGGCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 824 AGCGCT AGC^GCT
ApaI 108 GGGCCC GGGCC^C
ApaLI 1141 GTGCAC G^TGCAC
Asp700I 924 GAAGCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 33 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 48 CTCGAG C^[CT]CG[GA]G
BbeI 23 GGCGCC GGCGC^C
BbrPI 502 CACGTG CAC^GTG
BbvCI 195 GCTGAGG CCTCAGC(-5/-2)
BlpI 1048 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1048 GCTGAGC GC^T[ACGT]AGC
BsaBI 282 GATGCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 1067 GAGACC GGTCTC(1/5)
BsaWI 818 TCCGGA [AT]^CCGG[AT]
BseAI 818 TCCGGA T^CCGGA
BseRI 85 GAGGAG GAGGAG(10/8)
BsgI 499 CTGCAC GTGCAG(16/14)
BsiEI 6 CGACCG CG[GA][CT]^CG
BsmBI 164 GAGACG CGTCTC(1/5)
BsoBI 48 CTCGAG C^[CT]CG[GA]G
BspEI 818 TCCGGA T^CCGGA
BsrBI 804 CCGCTC CCGCTC(-3/-3)
BsrDI 296 GCAATG GCAATG(2/0)
BsrFI 169 GCCGGC [GA]^CCGG[CT]
BssSI 1243 CACGAG CACGAG(-5/-1)
CelII 1048 GCTGAGC GC^T[ACGT]AGC
Cfr10I 169 GCCGGC [GA]^CCGG[CT]
Eco47III 824 AGCGCT AGC^GCT
EcoT14I 668 CCAAGG C^C[AT][AT]GG
HincII 640 GTCAAC GT[CT]^[GA]AC
HindII 640 GTCAAC GT[CT]^[GA]AC
KasI 23 GGCGCC G^GCGCC
MaeIII 1108 GTCAC ^GT[ACGT]AC
MamI 282 GATGCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 818 TCCGGA T^CCGGA
NaeI 169 GCCGGC GCC^GGC
NarI 23 GGCGCC GG^CGCC
NgoMIV 169 GCCGGC G^CCGGC
PaeR7I 48 CTCGAG C^TCGAG
PflFI 33 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 502 CACGTG CAC^GTG
PmlI 502 CACGTG CAC^GTG
PvuII 1364 CAGCTG CAG^CTG
SbfI 210 CCTGCAGG CCTGCA^GG
SexAI 1250 ACCTGGT A^CC[AT]GGT
SfiI 507 GGCCAACCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 23 GGCGCC GGC^GCC
SgrAI 168 CGCCGGCG C[GA]^CCGG[CT]G
Sse8387I 210 CCTGCAGG CCTGCA^GG
StyI 668 CCAAGG C^C[AT][AT]GG
TfiI 217 GATTC G^A[AT]TC
Tsp45I 1108 GTCAC ^GT[GC]AC
Tth111I 33 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 1344 CCAGGGCAACTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 48 CTCGAG C^TCGAG
XmnI 924 GAAGCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 16 enzymes) #
###################################
AfaI 1248 GTAC GT^AC
1413 GTAC
BamHI 378 GGATCC G^GATCC
842 GGATCC
BsaAI 502 CACGTG [CT]AC^GT[GA]
1429 TACGTG
BsmAI 164 GAGAC GTCTC(1/5)
1067 GAGAC
BsmI 1038 GAATGC GAATGC(1/-1)
439 GCATTC
BstXI 350 CCATCCAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1315 CCACTGGCCTGG
DraII 108 GGGCCCC [GA]G^G[ACGT]CC[CT]
1445 AGGCCCC
DraIII 715 CACACCGTG CAC[ACGT][ACGT][ACGT]^GTG
777 CACCTTGTG
EcoO109I 108 GGGCCCC [GA]G^G[ACGT]CC[CT]
1445 AGGCCCC
KspI 9 CCGCGG CCGC^GG
125 CCGCGG
PleI 559 GAGTC GAGTC(4/5)
1102 GAGTC
PstI 211 CTGCAG CTGCA^G
680 CTGCAG
RsaI 1248 GTAC GT^AC
1413 GTAC
SacI 790 GAGCTC GAGCT^C
1051 GAGCTC
SacII 9 CCGCGG CCGC^GG
125 CCGCGG
SapI 621 GCTCTTC GCTCTTC(1/4)
1020 GCTCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 80 enzymes) #
#########################################
AccII AciI AcyI AluI AlwI
AspHI AvaII BalI BanI BanII
BbvI BcnI BglI BmyI BpmI
BsaHI BsaJI BsiHKAI BsiYI BslI
BsmFI Bsp1286I BsrI BssKI BstF5I
BstNI BstUI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DsaI EaeI
EarI EcoRII FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeII MboI MboII
MflI MluNI MnlI MscI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
TaqI TseI TspRI TthHB8I XhoII