Restriction map for
pFN21AE4512
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# NO CUT ENZYMES ( 134 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AcyI AflII AflIII AhdI
Alw44I ApaI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AviII AvrII BalI BamHI BbeI
BbrPI BcgI BclI BfrI BglII
BlnI BlpI Bpu1102I BsaAI BsaBI
BsaHI BseAI BsiEI BsiWI BsmBI
BspDI BspEI BspHI BspLU11I BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstYI BstZ17I CelII ClaI
CpoI DraI DraIII DrdI EaeI
EagI Eam1105I EarI EclXI Eco52I
EcoNI EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI FspI HgaI Hin1I
HindIII HpaI KasI KpnI Ksp632I
KspI MamI MfeI MflI MluI
MluNI MroI MscI MunI NaeI
NarI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PmaCI PmeI PmlI PshBI Psp1406I
PstI PvuI RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfaNI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspI SwaI XbaI XhoI
XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AccII 779 CGCG CG^CG
AfaI 1070 GTAC GT^AC
AgeI 461 ACCGGT A^CCGGT
Aor51HI 1037 AGCGCT AGC^GCT
AspHI 106 GAGCAC G[AT]GC[AT]^C
AspI 212 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BanI 906 GGTGCC G^G[CT][GA]CC
BbsI 1128 GAAGAC GAAGAC(2/6)
BbvCI 96 GCTGAGG CCTCAGC(-5/-2)
BciVI 1089 GGATAC GTATCC(6/5)
BglI 1189 GCCCGTCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 1128 GAAGAC GAAGAC(2/6)
BsaWI 461 ACCGGT [AT]^CCGG[AT]
BsiHKAI 106 GAGCAC G[AT]GC[AT]^C
Bsp1407I 1069 TGTACA T^GTACA
BsrBI 954 CCGCTC CCGCTC(-3/-3)
BsrDI 718 GCAATG GCAATG(2/0)
BsrGI 1069 TGTACA T^GTACA
BstUI 779 CGCG CG^CG
DraII 306 GGGTCCC [GA]G^G[ACGT]CC[CT]
DsaI 167 CCATGG C^C[GA][CT]GG
Eco47III 1037 AGCGCT AGC^GCT
EcoO109I 306 GGGTCCC [GA]G^G[ACGT]CC[CT]
HincII 1486 GTCAAC GT[CT]^[GA]AC
HindII 1486 GTCAAC GT[CT]^[GA]AC
MseI 1396 TTAA T^TAA
MvnI 779 CGCG CG^CG
NcoI 167 CCATGG C^CATGG
NspI 558 GCATGT [GA]CATG^[CT]
PflFI 212 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 483 CCACCGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 461 ACCGGT A^CCGGT
PpuMI 306 GGGTCCC [GA]G^G[AT]CC[CT]
PshAI 1237 GACATCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RsaI 1070 GTAC GT^AC
SfcI 80 CTACAG C^T[GA][CT]AG
SmlI 883 CTTGAG C^T[CT][GA]AG
SspBI 1069 TGTACA T^GTACA
TaqI 1245 TCGA T^CGA
TfiI 728 GATTC G^A[AT]TC
Tru9I 1396 TTAA T^TAA
Tth111I 212 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 1245 TCGA T^CGA
Van91I 483 CCACCGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 25 enzymes) #
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AlwNI 419 CAGAACCTG CAG[ACGT][ACGT][ACGT]^CTG
977 CAGCTACTG
AocI 319 CCTCAGG CC^T[ACGT]AGG
942 CCTGAGG
AvaI 876 CTCGGG C^[CT]CG[GA]G
1172 CTCGGG
BfaI 688 CTAG C^TAG
1525 CTAG
BsaI 622 GAGACC GGTCTC(1/5)
1442 GAGACC
BsmI 386 GAATGC GAATGC(1/-1)
435 GAATGC
BsoBI 876 CTCGGG C^[CT]CG[GA]G
1172 CTCGGG
BsrFI 461 ACCGGT [GA]^CCGG[CT]
485 ACCGGC
BsrI 767 ACTGG ACTGG(1/-1)
1576 ACTGG
BstXI 1256 CCAACCTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1269 CCACCTCTGTGG
Bsu36I 319 CCTCAGG CC^T[ACGT]AGG
942 CCTGAGG
CfoI 948 GCGC GCG^C
1038 GCGC
Cfr10I 461 ACCGGT [GA]^CCGG[CT]
485 ACCGGC
Eco81I 319 CCTCAGG CC^T[ACGT]AGG
942 CCTGAGG
FauI 653 CCCGC CCCGC(4/6)
1340 CCCGC
HaeII 947 GGCGCT [GA]GCGC^[CT]
1037 AGCGCT
HhaI 948 GCGC GCG^C
1038 GCGC
HinP1I 948 GCGC G^CGC
1038 GCGC
MaeI 688 CTAG C^TAG
1525 CTAG
MaeII 1428 ACGT A^CGT
1517 ACGT
MboII 1128 GAAGA GAAGA(8/7)
1615 GAAGA
MslI 364 CACCCCAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1270 CACCTCTGTG
PvuII 94 CAGCTG CAG^CTG
1018 CAGCTG
StuI 1079 AGGCCT AGG^CCT
1370 AGGCCT
XcmI 14 CCAGAGCAGCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
483 CCACCGGCTGGATGG
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# 3 OR MORE CUT ENZYMES ( 55 enzymes) #
#########################################
AciI AluI AlwI AvaII BanII
BbvI BcnI BmyI BpmI BsaJI
BseRI BsgI BsiYI BslI BsmAI
BsmFI Bsp1286I BspMI BssKI BstF5I
BstNI Cac8I Cfr13I DdeI DpnII
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HinfI HpaII HphI ItaI
MaeIII MboI MnlI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV PleI Sau3AI Sau96I ScrFI
StyI TseI Tsp45I Tsp509I TspRI