Restriction map for
pFN21AE9661
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# NO CUT ENZYMES ( 185 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AflII AflIII
AgeI AhdI Alw44I AocI Aor51HI
ApaI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspHI
AspI AvaI AviII AvrII BalI
BamHI BanI BanII BbeI BbrPI
BbsI BbvCI BcgI BciVI BcnI
BfaI BfrI BglI BglII BlnI
BmyI BpmI BpuAI BsaAI BsaBI
BsaHI BsaJI BsaWI BseAI BsgI
BsiEI BsiHKAI BsiWI BsmI BsoBI
Bsp1286I Bsp1407I BspDI BspEI BspHI
BspLU11I BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstXI
BstZ17I Bsu36I CfoI Cfr10I ClaI
CpoI DraII DraIII DrdI DsaI
EaeI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO109I EcoO65I
EcoRI EcoRV EcoT14I EcoT22I FauI
FseI FspI HaeII HapII HgaI
HhaI Hin1I HinP1I HindIII HpaII
KasI KpnI KspI MaeI MaeII
MamI MfeI MluI MluNI MroI
MscI MslI MspI MunI MvnI
MwoI NaeI NarI NciI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StyI SwaI Tth111I XbaI
XcmI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 31 enzymes) #
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AciI 439 CCGC CCGC(-3/-1)
AfaI 436 GTAC GT^AC
BclI 579 TGATCA T^GATCA
BlpI 682 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 682 GCTCAGC GC^T[ACGT]AGC
BsaI 71 GAGACC GGTCTC(1/5)
BsmBI 739 GAGACG CGTCTC(1/5)
BsmFI 646 GGGAC GGGAC(10/14)
CelII 682 GCTCAGC GC^T[ACGT]AGC
EarI 52 GAAGAG CTCTTC(1/4)
FbaI 579 TGATCA T^GATCA
HaeIII 760 GGCC GG^CC
HincII 240 GTTAAC GT[CT]^[GA]AC
HindII 240 GTTAAC GT[CT]^[GA]AC
HpaI 240 GTTAAC GTT^AAC
Ksp632I 52 GAAGAG CTCTTC(1/4)
MaeIII 563 GTGAC ^GT[ACGT]AC
MspA1I 482 CAGCTG C[AC]G^C[GT]G
NlaIV 646 GGGACC GG[ACGT]^[ACGT]CC
PflMI 75 CCAAACTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 445 GAGTC GAGTC(4/5)
PstI 25 CTGCAG CTGCA^G
PvuII 482 CAGCTG CAG^CTG
RsaI 436 GTAC GT^AC
SfcI 25 CTGCAG C^T[GA][CT]AG
StuI 759 AGGCCT AGG^CCT
TaqI 735 TCGA T^CGA
TfiI 36 GATTC G^A[AT]TC
Tsp45I 563 GTGAC ^GT[GC]AC
TthHB8I 735 TCGA T^CGA
Van91I 75 CCAAACTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AlwNI 177 CAGACACTG CAG[ACGT][ACGT][ACGT]^CTG
443 CAGAGTCTG
AvaII 185 GGACC G^G[AT]CC
647 GGACC
BbvI 608 GCAGC GCAGC(8/12)
24 GCTGC
BseRI 55 GAGGAG GAGGAG(10/8)
202 GAGGAG
BsiYI 75 CCAAACTTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
587 CCATGCTTAGG
BslI 75 CCAAACTTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
587 CCATGCTTAGG
BsmAI 71 GAGAC GTCTC(1/5)
739 GAGAC
BsrI 182 ACTGG ACTGG(1/-1)
399 CCAGT
BssKI 541 CCAGG ^CC[ACGT]GG
678 CCTGG
BstF5I 490 CATCC GGATG(2/0)
453 GGATG
BstNI 541 CCAGG CC^[AT]GG
678 CCTGG
BstYI 135 GGATCT [GA]^GATC[CT]
300 AGATCC
Cfr13I 185 GGACC G^G[ACGT]CC
647 GGACC
DraI 94 TTTAAA TTT^AAA
598 TTTAAA
EcoRII 541 CCAGG ^CC[AT]GG
678 CCTGG
Fnu4HI 24 GCTGC GC^[ACGT]GC
608 GCAGC
FokI 490 CATCC GGATG(9/13)
453 GGATG
HinfI 36 GATTC G^A[ACGT]TC
445 GAGTC
HphI 744 GGTGA GGTGA(8/7)
41 TCACC
ItaI 24 GCTGC GC^[ACGT]GC
608 GCAGC
MflI 135 GGATCT [GA]^GATC[CT]
300 AGATCC
MvaI 541 CCAGG CC^[AT]GG
678 CCTGG
Sau96I 185 GGACC G^G[ACGT]CC
647 GGACC
ScrFI 541 CCAGG CC^[ACGT]GG
678 CCTGG
SfaNI 386 GATGC GCATC(5/9)
501 GATGC
TseI 24 GCTGC G^C[AT]GC
608 GCAGC
TspRI 181 CACTG CA[GC]TG[ACGT][ACGT]^
400 CAGTG
XhoII 135 GGATCT [GA]^GATC[CT]
300 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 14 enzymes) #
#########################################
AluI AlwI Cac8I DdeI DpnII
MboI MboII MnlI MseI NdeII
NlaIII Sau3AI Tru9I Tsp509I