Restriction map for
pFN21AE6413
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# NO CUT ENZYMES ( 136 enzymes) #
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AatII AccII AccIII AclI AcyI
AflII AflIII AgeI AhdI Alw44I
AlwI AocI Aor51HI ApaI ApaLI
AscI Asp700I AspEI AspHI AviII
AvrII BamHI BbeI BbrPI BbvCI
BcgI BclI BfrI BlnI BlpI
BpmI Bpu1102I BsaAI BsaHI BsaWI
BseAI BsiEI BsiHKAI BsiWI BsmBI
Bsp1407I BspDI BspEI BspLU11I BspMI
BsrBI BsrGI BssHII BssSI Bst1107I
BstBI BstUI BstZ17I Bsu36I CelII
CfoI ClaI CpoI DraI DraIII
DrdI DsaI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoRI EcoRV
FbaI FseI FspI HaeII HgaI
HhaI Hin1I HinP1I HindIII HpaI
KasI KspI MaeII MfeI MluI
MroI MspA1I MunI MvnI NaeI
NarI NcoI NdeI NgoMIV NheI
NotI NruI NspV PacI PaeR7I
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI Psp1406I PstI PvuI
PvuII RsrII SacI SacII SapI
SbfI ScaI SexAI SfcI SfiI
SfoI SfuI SgrAI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI Van91I XbaI XhoI
XmnI
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# ONE CUT ENZYMES ( 44 enzymes) #
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Acc65I 54 GGTACC G^GTACC
AccI 757 GTCGAC GT^[AC][GT]AC
AciI 459 CCGC CCGC(-3/-1)
Asp718I 54 GGTACC G^GTACC
AspI 739 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BciVI 1135 GTATCC GTATCC(6/5)
BfaI 1024 CTAG C^TAG
BglI 592 GCCTCACAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 266 AGATCT A^GATCT
BsaBI 1062 GATGGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI 169 GAGGAG GAGGAG(10/8)
BsmFI 438 GGGAC GGGAC(10/14)
BsmI 1289 GAATGC GAATGC(1/-1)
BsrDI 587 GCAATG GCAATG(2/0)
BsrFI 1250 ACCGGC [GA]^CCGG[CT]
BstAPI 1044 GCATGAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 919 GGTGACC G^GT[ACGT]ACC
BstF5I 501 CATCC GGATG(2/0)
BstPI 919 GGTGACC G^GT[ACGT]ACC
BstYI 266 AGATCT [GA]^GATC[CT]
Cfr10I 1250 ACCGGC [GA]^CCGG[CT]
DraII 392 AGGCCCT [GA]G^G[ACGT]CC[CT]
EcoO109I 392 AGGCCCT [GA]G^G[ACGT]CC[CT]
EcoO65I 919 GGTGACC G^GT[ACGT]ACC
EcoT22I 1042 ATGCAT ATGCA^T
FauI 458 CCCGC CCCGC(4/6)
FokI 501 CATCC GGATG(9/13)
KpnI 54 GGTACC GGTAC^C
MaeI 1024 CTAG C^TAG
MaeIII 920 GTGAC ^GT[ACGT]AC
MamI 1062 GATGGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 266 AGATCT [GA]^GATC[CT]
NsiI 1042 ATGCAT ATGCA^T
NspI 311 GCATGT [GA]CATG^[CT]
PflFI 739 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
SalI 757 GTCGAC G^TCGAC
SmaI 412 CCCGGG CCC^GGG
TaqI 758 TCGA T^CGA
Tsp45I 920 GTGAC ^GT[GC]AC
Tth111I 739 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 758 TCGA T^CGA
XhoII 266 AGATCT [GA]^GATC[CT]
XmaCI 412 CCCGGG C^CCGGG
XmaI 412 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AlwNI 391 CAGGCCCTG CAG[ACGT][ACGT][ACGT]^CTG
1112 CAGCTACTG
AseI 670 ATTAAT AT^TAAT
687 ATTAAT
AsnI 670 ATTAAT AT^TAAT
687 ATTAAT
AvaI 412 CCCGGG C^[CT]CG[GA]G
638 CTCGGG
AvaII 900 GGTCC G^G[AT]CC
1248 GGACC
BalI 206 TGGCCA TGG^CCA
320 TGGCCA
BanII 379 GGGCTC G[GA]GC[CT]^C
415 GGGCTC
BsaI 88 GAGACC GGTCTC(1/5)
448 GAGACC
BsgI 124 GTGCAG GTGCAG(16/14)
812 GTGCAG
BsoBI 412 CCCGGG C^[CT]CG[GA]G
638 CTCGGG
BspHI 611 TCATGA T^CATGA
1265 TCATGA
EarI 142 GAAGAG CTCTTC(1/4)
187 GAAGAG
HincII 726 GTTGAC GT[CT]^[GA]AC
757 GTCGAC
HindII 726 GTTGAC GT[CT]^[GA]AC
757 GTCGAC
Ksp632I 142 GAAGAG CTCTTC(1/4)
187 GAAGAG
MluNI 206 TGGCCA TGG^CCA
320 TGGCCA
MscI 206 TGGCCA TGG^CCA
320 TGGCCA
PshBI 670 ATTAAT AT^TAAT
687 ATTAAT
RcaI 611 TCATGA T^CATGA
1265 TCATGA
SfaNI 336 GATGC GCATC(5/9)
1041 GATGC
XcmI 887 CCACCATGCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
950 CCACAGACTTGGTGG
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# 3 OR MORE CUT ENZYMES ( 57 enzymes) #
#########################################
AcsI AfaI AluI ApoI BanI
BbsI BbvI BcnI BmyI BpuAI
BsaJI BsiYI BslI BsmAI Bsp1286I
BsrI BssKI BstNI BstXI Cac8I
Cfr13I DdeI DpnII EaeI EcoNI
EcoRII EcoT14I Fnu4HI HaeIII HapII
HinfI HpaII HphI ItaI MboI
MboII MnlI MseI MslI MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV PleI RsaI Sau3AI Sau96I
ScrFI StyI TfiI Tru9I TseI
Tsp509I TspRI