Restriction map for
pFN21AE6306
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# NO CUT ENZYMES ( 146 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcsI AcyI AfaI AflII AgeI
AhdI Alw44I AocI Aor51HI ApaI
ApaLI ApoI AscI AseI AsnI
Asp718I AspEI AspI AvaI AviII
AvrII BamHI BbeI BbrPI BbsI
BbvCI BcgI BciVI BfaI BfrI
BglII BlnI BpuAI BsaAI BsaBI
BsaHI BsaWI BseAI BseRI BsiEI
BsiWI BsmBI BsoBI Bsp1407I BspEI
BspHI BsrGI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstUI BstYI
BstZ17I Bsu36I CpoI DraI DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoRI
EcoRV EcoT22I FseI FspI HgaI
Hin1I HindIII HpaI KasI KpnI
KspI MaeI MaeII MaeIII MamI
MfeI MflI MluI MroI MunI
MvnI NarI NcoI NdeI NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PstI PvuI RcaI RsaI RsrII
SacII SalI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI Tsp45I Tth111I
Van91I XbaI XhoI XhoII XmaCI
XmaI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AccI 946 GTCTAC GT^[AC][GT]AC
AflIII 494 ACATGT A^C[GA][CT]GT
AlwI 695 GGATC GGATC(4/5)
Asp700I 72 GAAGCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 595 GAGCTC G[AT]GC[AT]^C
BalI 473 TGGCCA TGG^CCA
BanII 595 GAGCTC G[GA]GC[CT]^C
BclI 299 TGATCA T^GATCA
BcnI 650 CCCGG CC^[GC]GG
BglI 544 GCCGCTGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 807 GCTGAGC GC^T[ACGT]AGC
BpmI 319 CTCCAG CTGGAG(16/14)
Bpu1102I 807 GCTGAGC GC^T[ACGT]AGC
BsaI 961 GGTCTC GGTCTC(1/5)
BsgI 784 CTGCAC GTGCAG(16/14)
BsiHKAI 595 GAGCTC G[AT]GC[AT]^C
BsmAI 962 GTCTC GTCTC(1/5)
BsmFI 44 GGGAC GGGAC(10/14)
BsmI 405 GAATGC GAATGC(1/-1)
BspDI 28 ATCGAT AT^CGAT
BspLU11I 494 ACATGT A^CATGT
BspMI 798 GCAGGT ACCTGC(4/8)
BsrDI 794 CATTGC GCAATG(2/0)
BsrFI 339 GCCGGC [GA]^CCGG[CT]
CelII 807 GCTGAGC GC^T[ACGT]AGC
Cfr10I 339 GCCGGC [GA]^CCGG[CT]
ClaI 28 ATCGAT AT^CGAT
DraII 779 AGGTCCT [GA]G^G[ACGT]CC[CT]
DsaI 476 CCACGG C^C[GA][CT]GG
EarI 153 GAAGAG CTCTTC(1/4)
EcoO109I 779 AGGTCCT [GA]G^G[ACGT]CC[CT]
FbaI 299 TGATCA T^GATCA
HaeII 510 AGCGCC [GA]GCGC^[CT]
Ksp632I 153 GAAGAG CTCTTC(1/4)
MluNI 473 TGGCCA TGG^CCA
MscI 473 TGGCCA TGG^CCA
MseI 23 TTAA T^TAA
NaeI 339 GCCGGC GCC^GGC
NciI 650 CCCGG CC^[GC]GG
NgoMIV 339 GCCGGC G^CCGGC
PleI 223 GACTC GAGTC(4/5)
PpuMI 779 AGGTCCT [GA]G^G[AT]CC[CT]
PvuII 196 CAGCTG CAG^CTG
SacI 595 GAGCTC GAGCT^C
SapI 153 GAAGAGC GCTCTTC(1/4)
SfcI 327 CTACAG C^T[GA][CT]AG
TaqI 29 TCGA T^CGA
TfiI 608 GAATC G^A[AT]TC
Tru9I 23 TTAA T^TAA
TthHB8I 29 TCGA T^CGA
XmnI 72 GAAGCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 20 enzymes) #
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AlwNI 196 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
707 CAGCTCCTG
BsrBI 118 GAGCGG CCGCTC(-3/-3)
810 GAGCGG
BstAPI 759 GCACTTCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
798 GCAGGTCATGC
BstF5I 161 GGATG GGATG(2/0)
716 GGATG
Cac8I 339 GCCGGC GC[ACGT]^[ACGT]GC
786 GCACGC
EaeI 473 TGGCCA [CT]^GGCC[GA]
662 CGGCCA
EcoT14I 137 CCAAGG C^C[AT][AT]GG
518 CCTTGG
FokI 161 GGATG GGATG(9/13)
716 GGATG
HapII 340 CCGG C^CGG
651 CCGG
HincII 388 GTCAAC GT[CT]^[GA]AC
498 GTTGAC
HindII 388 GTCAAC GT[CT]^[GA]AC
498 GTTGAC
HinfI 223 GACTC G^A[ACGT]TC
608 GAATC
HpaII 340 CCGG C^CGG
651 CCGG
MnlI 822 CCTC CCTC(7/6)
4 GAGG
MspI 340 CCGG C^CGG
651 CCGG
NspI 272 GCATGT [GA]CATG^[CT]
494 ACATGT
SmlI 748 CTTGAG C^T[CT][GA]AG
934 CTTGAG
StyI 137 CCAAGG C^C[AT][AT]GG
518 CCTTGG
Tsp509I 755 AATT ^AATT
872 AATT
XcmI 137 CCAAGGTGATGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
234 CCACCCCGACACTGG
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# 3 OR MORE CUT ENZYMES ( 41 enzymes) #
#########################################
AciI AluI AvaII BanI BbvI
BmyI BsaJI BsiYI BslI Bsp1286I
BsrI BssKI BstNI BstXI CfoI
Cfr13I DdeI DpnII EcoRII FauI
Fnu4HI HaeIII HhaI HinP1I HphI
ItaI MboI MboII MslI MspA1I
MvaI MwoI NdeII NlaIII NlaIV
Sau3AI Sau96I ScrFI SfaNI TseI
TspRI