Restriction map for
pFN21AE6314
##################################
# NO CUT ENZYMES ( 146 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AflII AflIII AgeI AhdI Alw44I
AlwNI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp718I AspEI AspHI
AspI AvaI AviII BalI BamHI
BbrPI BbvCI BcgI BciVI BcnI
BfrI BglI BlpI Bpu1102I BsaAI
BsaBI BsaI BseAI BsiEI BsiHKAI
BsiWI BsmBI BsmFI BsmI BsoBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstEII
BstPI BstUI BstZ17I CelII Cfr10I
ClaI CpoI DraI DrdI EaeI
EagI Eam1105I EclXI Eco47III Eco52I
EcoNI EcoO65I EcoRV EcoT22I FauI
FseI FspI HgaI HincII HindII
HindIII HpaI KpnI KspI MamI
MfeI MluI MluNI MroI MscI
MspA1I MunI MvnI NaeI NciI
NgoMIV NheI NotI NruI NsiI
NspI PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PstI PvuI PvuII
RcaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfcI
SfiI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI SwaI
Tth111I Van91I XbaI XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 56 enzymes) #
###################################
AccI 347 GTAGAC GT^[AC][GT]AC
AcyI 883 GGCGCC G[GA]^CG[CT]C
AfaI 495 GTAC GT^AC
AocI 639 CCTAAGG CC^T[ACGT]AGG
AvaII 652 GGACC G^G[AT]CC
BanII 265 GGGCTC G[GA]GC[CT]^C
BbeI 883 GGCGCC GGCGC^C
BclI 564 TGATCA T^GATCA
BpmI 832 CTCCAG CTGGAG(16/14)
BsaHI 883 GGCGCC G[GA]^CG[CT]C
BsaWI 37 ACCGGA [AT]^CCGG[AT]
BsmAI 778 GAGAC GTCTC(1/5)
BssKI 353 CCAGG ^CC[ACGT]GG
BstBI 718 TTCGAA TT^CGAA
BstNI 353 CCAGG CC^[AT]GG
Bsu36I 639 CCTAAGG CC^T[ACGT]AGG
Cac8I 634 GCTGGC GC[ACGT]^[ACGT]GC
CfoI 884 GCGC GCG^C
Cfr13I 652 GGACC G^G[ACGT]CC
DdeI 640 CTAAG C^T[ACGT]AG
DraII 651 AGGACCC [GA]G^G[ACGT]CC[CT]
DraIII 75 CACATGGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 243 CCATGG C^C[GA][CT]GG
Eco81I 639 CCTAAGG CC^T[ACGT]AGG
EcoO109I 651 AGGACCC [GA]G^G[ACGT]CC[CT]
EcoRI 1006 GAATTC G^AATTC
EcoRII 353 CCAGG ^CC[AT]GG
FbaI 564 TGATCA T^GATCA
HaeII 883 GGCGCC [GA]GCGC^[CT]
HapII 38 CCGG C^CGG
HhaI 884 GCGC GCG^C
Hin1I 883 GGCGCC G[GA]^CG[CT]C
HinP1I 884 GCGC G^CGC
HpaII 38 CCGG C^CGG
HphI 775 GGTGA GGTGA(8/7)
KasI 883 GGCGCC G^GCGCC
MaeII 88 ACGT A^CGT
MaeIII 733 GTGAC ^GT[ACGT]AC
MspI 38 CCGG C^CGG
MvaI 353 CCAGG CC^[AT]GG
NarI 883 GGCGCC GG^CGCC
NcoI 243 CCATGG C^CATGG
NdeI 944 CATATG CA^TATG
NspV 718 TTCGAA TT^CGAA
PpuMI 651 AGGACCC [GA]G^G[AT]CC[CT]
RsaI 495 GTAC GT^AC
Sau96I 652 GGACC G^G[ACGT]CC
ScrFI 353 CCAGG CC^[ACGT]GG
SfoI 883 GGCGCC GGC^GCC
SfuI 718 TTCGAA TT^CGAA
SspI 211 AATATT AAT^ATT
StuI 685 AGGCCT AGG^CCT
TaqI 719 TCGA T^CGA
Tsp45I 733 GTGAC ^GT[GC]AC
TthHB8I 719 TCGA T^CGA
XcmI 577 CCAATTCCAGTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AciI 4 GCGG CCGC(-3/-1)
7 GCGG
AcsI 279 GAATTT [GA]^AATT[CT]
1006 GAATTC
AlwI 890 GATCC GGATC(4/5)
590 GGATC
ApoI 279 GAATTT [GA]^AATT[CT]
1006 GAATTC
Asp700I 157 GAACCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
701 GAAAGTATTC
AvrII 423 CCTAGG C^CTAGG
459 CCTAGG
BbsI 721 GAAGAC GAAGAC(2/6)
951 GAAGAC
BglII 288 AGATCT A^GATCT
1028 AGATCT
BlnI 423 CCTAGG C^CTAGG
459 CCTAGG
BpuAI 721 GAAGAC GAAGAC(2/6)
951 GAAGAC
BsgI 673 GTGCAG GTGCAG(16/14)
760 GTGCAG
BstXI 243 CCATGGTTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
834 CCAGCGAATTGG
BstYI 288 AGATCT [GA]^GATC[CT]
1028 AGATCT
EarI 115 GAAGAG CTCTTC(1/4)
406 GAAGAG
Ksp632I 115 GAAGAG CTCTTC(1/4)
406 GAAGAG
MflI 288 AGATCT [GA]^GATC[CT]
1028 AGATCT
NlaIII 77 CATG CATG^
244 CATG
SfaNI 367 GATGC GCATC(5/9)
920 GCATC
XhoII 288 AGATCT [GA]^GATC[CT]
1028 AGATCT
XmnI 157 GAACCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
701 GAAAGTATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 36 enzymes) #
#########################################
AluI BanI BbvI BfaI BmyI
BsaJI BseRI BsiYI BslI Bsp1286I
BsrI BstF5I DpnII EcoT14I Fnu4HI
FokI HaeIII HinfI ItaI MaeI
MboI MboII MnlI MseI MslI
MwoI NdeII NlaIV PleI Sau3AI
StyI TfiI Tru9I TseI Tsp509I
TspRI