Restriction map for
pFN21AE3926
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# NO CUT ENZYMES ( 93 enzymes) #
##################################
Acc65I AccI AclI AcsI AflII
AflIII AgeI AhdI AocI Aor51HI
ApoI AscI AseI AsnI Asp718I
AspEI AviII AvrII BamHI BbsI
BbvCI BclI BfrI BglII BlnI
BlpI Bpu1102I BpuAI BsaBI BsaI
BsmI BspDI BspLU11I BsrDI Bst1107I
BstBI BstEII BstPI BstZ17I Bsu36I
CelII ClaI CpoI DraI DrdI
Eam1105I Eco47III Eco81I EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HincII HindII HindIII HpaI KpnI
MamI MfeI MluI MseI MunI
NdeI NheI NruI NsiI NspV
PacI PinAI PmeI PshAI PshBI
Psp1406I PvuI RsrII SalI SbfI
SexAI SfiI SfuI SmaI SnaBI
SpeI Sse8387I SspI SwaI Tru9I
XbaI XmaCI XmaI
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# ONE CUT ENZYMES ( 54 enzymes) #
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AatII 1170 GACGTC GACGT^C
AccIII 566 TCCGGA T^CCGGA
Alw44I 1303 GTGCAC G^TGCAC
AlwI 549 GATCC GGATC(4/5)
ApaLI 1303 GTGCAC G^TGCAC
Asp700I 18 GAACTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 1170 GACGTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BcgI 1388 GCAAGATTCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1335 GTATCC GTATCC(6/5)
BsaWI 566 TCCGGA [AT]^CCGG[AT]
BseAI 566 TCCGGA T^CCGGA
BsiEI 1383 CGGCCG CG[GA][CT]^CG
BsiWI 147 CGTACG C^GTACG
BsmBI 1168 GAGACG CGTCTC(1/5)
Bsp1407I 632 TGTACA T^GTACA
BspEI 566 TCCGGA T^CCGGA
BsrGI 632 TGTACA T^GTACA
BssHII 515 GCGCGC G^CGCGC
BssSI 945 CACGAG CACGAG(-5/-1)
BstF5I 882 GGATG GGATG(2/0)
BstXI 215 CCAACATCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 548 AGATCC [GA]^GATC[CT]
DdeI 968 CTAAG C^T[ACGT]AG
EagI 1383 CGGCCG C^GGCCG
EarI 994 CTCTTC CTCTTC(1/4)
EclXI 1383 CGGCCG C^GGCCG
Eco52I 1383 CGGCCG C^GGCCG
FokI 882 GGATG GGATG(9/13)
Ksp632I 994 CTCTTC CTCTTC(1/4)
MflI 548 AGATCC [GA]^GATC[CT]
MroI 566 TCCGGA T^CCGGA
MslI 1059 CACGCCGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 258 GCCGGC GCC^GGC
NcoI 1440 CCATGG C^CATGG
NgoMIV 258 GCCGGC G^CCGGC
NotI 1382 GCGGCCGC GC^GGCCGC
NspI 824 GCATGC [GA]CATG^[CT]
PaeR7I 640 CTCGAG C^TCGAG
PflFI 1170 GACGTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 720 CCAGCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 643 GAGTC GAGTC(4/5)
SacI 34 GAGCTC GAGCT^C
SapI 993 GCTCTTC GCTCTTC(1/4)
SphI 824 GCATGC GCATG^C
SspBI 632 TGTACA T^GTACA
StuI 170 AGGCCT AGG^CCT
TfiI 1392 GATTC G^A[AT]TC
Tsp45I 1645 GTGAC ^GT[GC]AC
Tsp509I 10 AATT ^AATT
Tth111I 1170 GACGTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 720 CCAGCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 640 CTCGAG C^TCGAG
XhoII 548 AGATCC [GA]^GATC[CT]
XmnI 18 GAACTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 37 enzymes) #
###################################
ApaI 1179 GGGCCC GGGCC^C
1249 GGGCCC
AspHI 34 GAGCTC G[AT]GC[AT]^C
1303 GTGCAC
AvaI 640 CTCGAG C^[CT]CG[GA]G
814 CTCGGG
BalI 1010 TGGCCA TGG^CCA
1433 TGGCCA
BbeI 333 GGCGCC GGCGC^C
1291 GGCGCC
BbrPI 244 CACGTG CAC^GTG
352 CACGTG
BsiHKAI 34 GAGCTC G[AT]GC[AT]^C
1303 GTGCAC
BsmAI 1168 GAGAC GTCTC(1/5)
1565 GAGAC
BsoBI 640 CTCGAG C^[CT]CG[GA]G
814 CTCGGG
BspHI 578 TCATGA T^CATGA
893 TCATGA
BsrBI 1464 CCGCTC CCGCTC(-3/-3)
1513 CCGCTC
DpnII 549 GATC ^GATC
972 GATC
EcoT14I 131 CCAAGG C^C[AT][AT]GG
1440 CCATGG
HgaI 360 GACGC GACGC(5/10)
1576 GACGC
HinfI 643 GAGTC G^A[ACGT]TC
1392 GATTC
HphI 1222 GGTGA GGTGA(8/7)
212 TCACC
KasI 333 GGCGCC G^GCGCC
1291 GGCGCC
KspI 288 CCGCGG CCGC^GG
387 CCGCGG
MaeIII 1418 GTTAC ^GT[ACGT]AC
1645 GTGAC
MboI 549 GATC ^GATC
972 GATC
MluNI 1010 TGGCCA TGG^CCA
1433 TGGCCA
MscI 1010 TGGCCA TGG^CCA
1433 TGGCCA
NarI 333 GGCGCC GG^CGCC
1291 GGCGCC
NdeII 549 GATC ^GATC
972 GATC
PmaCI 244 CACGTG CAC^GTG
352 CACGTG
PmlI 244 CACGTG CAC^GTG
352 CACGTG
RcaI 578 TCATGA T^CATGA
893 TCATGA
SacII 288 CCGCGG CCGC^GG
387 CCGCGG
Sau3AI 549 GATC ^GATC
972 GATC
ScaI 494 AGTACT AGT^ACT
1502 AGTACT
SfoI 333 GGCGCC GGC^GCC
1291 GGCGCC
SgrAI 257 CGCCGGCG C[GA]^CCGG[CT]G
1061 CGCCGGTG
SmlI 640 CTCGAG C^T[CT][GA]AG
1308 CTCAAG
StyI 131 CCAAGG C^C[AT][AT]GG
1440 CCATGG
TaqI 26 TCGA T^CGA
641 TCGA
TthHB8I 26 TCGA T^CGA
641 TCGA
XcmI 836 CCAAGAAGCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1262 CCACGCAGCACTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 74 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwNI AvaII BanI BanII BbvI
BcnI BfaI BglI BmyI BpmI
BsaAI BsaHI BsaJI BseRI BsgI
BsiYI BslI BsmFI Bsp1286I BspMI
BsrFI BsrI BssKI BstAPI BstNI
BstUI Cac8I CfoI Cfr10I Cfr13I
DraII DraIII DsaI EaeI EcoNI
EcoO109I EcoRII FauI Fnu4HI HaeII
HaeIII HapII HhaI Hin1I HinP1I
HpaII ItaI MaeI MaeII MboII
MnlI MspA1I MspI MvaI MvnI
MwoI NciI NlaIII NlaIV PpuMI
PstI PvuII RsaI Sau96I ScrFI
SfaNI SfcI TseI TspRI