Restriction map for
pFN21AE6708
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# NO CUT ENZYMES ( 98 enzymes) #
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AatII Acc65I AccI AclI AcsI
AflII AgeI AhdI Alw44I ApaLI
ApoI AscI AseI AsnI Asp718I
AspEI AviII AvrII BbrPI BcgI
BciVI BclI BfaI BfrI BglI
BglII BlnI BsaBI BsiWI BsmBI
BsmI BspHI BsrBI BsrFI BssHII
Bst1107I BstBI BstEII BstPI BstZ17I
Cfr10I CpoI DraI Eam1105I EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
FspI HindIII HpaI KpnI MaeI
MamI MfeI MluI MseI MslI
MunI NaeI NdeI NgoMIV NheI
NotI NsiI NspV PacI PaeR7I
PflMI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SalI SbfI ScaI SexAI
SfiI SfuI SgrAI SnaBI SpeI
Sse8387I SspI StuI SwaI Tru9I
Van91I XbaI XhoI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AccIII 599 TCCGGA T^CCGGA
AlwNI 1198 CAGTGGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 1179 CCTGAGG CC^T[ACGT]AGG
Aor51HI 1460 AGCGCT AGC^GCT
ApaI 1154 GGGCCC GGGCC^C
BalI 779 TGGCCA TGG^CCA
BamHI 93 GGATCC G^GATCC
BbeI 36 GGCGCC GGCGC^C
BbsI 1171 GAAGAC GAAGAC(2/6)
BlpI 1951 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1951 GCTGAGC GC^T[ACGT]AGC
BpuAI 1171 GAAGAC GAAGAC(2/6)
BsaAI 1792 TACGTG [CT]AC^GT[GA]
BsaI 1567 GAGACC GGTCTC(1/5)
BsaWI 599 TCCGGA [AT]^CCGG[AT]
BseAI 599 TCCGGA T^CCGGA
BspDI 1855 ATCGAT AT^CGAT
BspEI 599 TCCGGA T^CCGGA
BspLU11I 701 ACATGT A^CATGT
BsrDI 902 GCAATG GCAATG(2/0)
BssSI 1142 CACGAG CACGAG(-5/-1)
BstAPI 462 GCAGCGGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 1179 CCTGAGG CC^T[ACGT]AGG
CelII 1951 GCTGAGC GC^T[ACGT]AGC
ClaI 1855 ATCGAT AT^CGAT
DraIII 175 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 2056 GACGACCTCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 1460 AGCGCT AGC^GCT
Eco81I 1179 CCTGAGG CC^T[ACGT]AGG
HincII 2047 GTCAAC GT[CT]^[GA]AC
HindII 2047 GTCAAC GT[CT]^[GA]AC
KasI 36 GGCGCC G^GCGCC
MluNI 779 TGGCCA TGG^CCA
MroI 599 TCCGGA T^CCGGA
MscI 779 TGGCCA TGG^CCA
NarI 36 GGCGCC GG^CGCC
NruI 342 TCGCGA TCG^CGA
PpuMI 294 GGGACCT [GA]G^G[AT]CC[CT]
SacI 1954 GAGCTC GAGCT^C
SfoI 36 GGCGCC GGC^GCC
SmaI 1253 CCCGGG CCC^GGG
TfiI 1801 GAATC G^A[AT]TC
Tsp509I 929 AATT ^AATT
XmaCI 1253 CCCGGG C^CCGGG
XmaI 1253 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 26 enzymes) #
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AflIII 701 ACATGT A^C[GA][CT]GT
1494 ACGTGT
Asp700I 687 GAAGCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2092 GAAGATGTTC
AspHI 1435 GAGCAC G[AT]GC[AT]^C
1954 GAGCTC
AvaI 229 CCCGAG C^[CT]CG[GA]G
1253 CCCGGG
BbvCI 1641 CCTCAGC CCTCAGC(-5/-2)
1087 GCTGAGG
BsiEI 30 CGGCCG CG[GA][CT]^CG
967 CGGCCG
BsiHKAI 1435 GAGCAC G[AT]GC[AT]^C
1954 GAGCTC
BsmAI 721 GAGAC GTCTC(1/5)
1567 GAGAC
BsoBI 229 CCCGAG C^[CT]CG[GA]G
1253 CCCGGG
Bsp1407I 182 TGTACA T^GTACA
419 TGTACA
BsrGI 182 TGTACA T^GTACA
419 TGTACA
DraII 294 GGGACCT [GA]G^G[ACGT]CC[CT]
1154 GGGCCCT
EagI 30 CGGCCG C^GGCCG
967 CGGCCG
EclXI 30 CGGCCG C^GGCCG
967 CGGCCG
Eco52I 30 CGGCCG C^GGCCG
967 CGGCCG
EcoNI 1215 CCTGCATCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2061 CCTCGTCAAGG
EcoO109I 294 GGGACCT [GA]G^G[ACGT]CC[CT]
1154 GGGCCCT
HgaI 4 GCGTC GACGC(5/10)
1163 GCGTC
KspI 27 CCGCGG CCGC^GG
76 CCGCGG
NcoI 259 CCATGG C^CATGG
495 CCATGG
PleI 349 GACTC GAGTC(4/5)
723 GACTC
SacII 27 CCGCGG CCGC^GG
76 CCGCGG
SapI 2211 GAAGAGC GCTCTTC(1/4)
690 GCTCTTC
SspBI 182 TGTACA T^GTACA
419 TGTACA
XcmI 800 CCATCGAGGAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2108 CCACCAGCCAGGTGG
XmnI 687 GAAGCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2092 GAAGATGTTC
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# 3 OR MORE CUT ENZYMES ( 89 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AspI AvaII BanI BanII
BbvI BcnI BmyI BpmI BsaHI
BsaJI BseRI BsgI BsiYI BslI
BsmFI Bsp1286I BspMI BsrI BssKI
BstF5I BstNI BstUI BstXI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DsaI EaeI EarI EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeII
MaeIII MboI MboII MflI MnlI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV NspI
PflFI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SphI StyI TaqI TseI Tsp45I
TspRI Tth111I TthHB8I XhoII