Restriction map for
pFN21AE1336
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# NO CUT ENZYMES ( 145 enzymes) #
##################################
Acc65I AccI AccIII AclI AflII
AflIII AgeI AhdI Alw44I AlwNI
Aor51HI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AvaI AviII
AvrII BalI BamHI BbeI BbrPI
BbsI BbvCI BcgI BciVI BclI
BfrI BglI BglII BlnI BlpI
Bpu1102I BpuAI BsaAI BsaBI BsaI
BsaWI BseAI BsiEI BsiWI BsmAI
BsmBI BsmI BsoBI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrBI BsrFI
BsrGI BssHII Bst1107I BstBI BstXI
BstZ17I CelII CfoI Cfr10I ClaI
CpoI DraI DraIII DrdI EaeI
EagI Eam1105I EclXI Eco47III Eco52I
EcoNI EcoRI EcoRV EcoT22I FbaI
FseI FspI HaeII HhaI HinP1I
HindIII HinfI KasI KpnI MamI
MfeI MluI MluNI MroI MscI
MunI NaeI NarI NcoI NdeI
NgoMIV NotI NruI NsiI NspV
PacI PaeR7I PflMI PinAI PleI
PmaCI PmeI PmlI PpuMI PshBI
Psp1406I PvuI PvuII RcaI RsrII
SalI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI Sse8387I SspBI SspI
SwaI TaqI TfiI TthHB8I Van91I
XbaI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 61 enzymes) #
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AatII 52 GACGTC GACGT^C
AccII 547 CGCG CG^CG
AcsI 373 AAATTC [GA]^AATT[CT]
AfaI 684 GTAC GT^AC
AocI 729 CCTCAGG CC^T[ACGT]AGG
ApaI 154 GGGCCC GGGCC^C
ApoI 373 AAATTC [GA]^AATT[CT]
AspHI 244 GAGCTC G[AT]GC[AT]^C
AspI 3 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaII 543 GGACC G^G[AT]CC
BanI 294 GGTGCC G^G[CT][GA]CC
BcnI 240 CCGGG CC^[GC]GG
BfaI 421 CTAG C^TAG
BpmI 592 CTCCAG CTGGAG(16/14)
BseRI 364 GAGGAG GAGGAG(10/8)
BsgI 70 GTGCAG GTGCAG(16/14)
BsiHKAI 244 GAGCTC G[AT]GC[AT]^C
BsrDI 535 GCAATG GCAATG(2/0)
BssSI 604 CACGAG CACGAG(-5/-1)
BstAPI 555 GCAGCTCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 621 GGTCACC G^GT[ACGT]ACC
BstPI 621 GGTCACC G^GT[ACGT]ACC
BstUI 547 CGCG CG^CG
BstYI 275 AGATCC [GA]^GATC[CT]
Bsu36I 729 CCTCAGG CC^T[ACGT]AGG
DdeI 730 CTCAG C^T[ACGT]AG
Eco81I 729 CCTCAGG CC^T[ACGT]AGG
EcoO65I 621 GGTCACC G^GT[ACGT]ACC
EcoT14I 641 CCTTGG C^C[AT][AT]GG
FauI 150 GCGGG CCCGC(4/6)
HgaI 76 GCGTC GACGC(5/10)
HincII 121 GTTAAC GT[CT]^[GA]AC
HindII 121 GTTAAC GT[CT]^[GA]AC
HpaI 121 GTTAAC GTT^AAC
HphI 623 TCACC GGTGA(8/7)
KspI 546 CCGCGG CCGC^GG
MaeI 421 CTAG C^TAG
MaeII 53 ACGT A^CGT
MflI 275 AGATCC [GA]^GATC[CT]
MseI 122 TTAA T^TAA
MvnI 547 CGCG CG^CG
NciI 240 CCGGG CC^[GC]GG
NheI 420 GCTAGC G^CTAGC
NspI 551 GCATGC [GA]CATG^[CT]
PflFI 3 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 432 GACCATTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 487 CTGCAG CTGCA^G
RsaI 684 GTAC GT^AC
SacI 244 GAGCTC GAGCT^C
SacII 546 CCGCGG CCGC^GG
SapI 246 GCTCTTC GCTCTTC(1/4)
SfaNI 586 GATGC GCATC(5/9)
SfcI 487 CTGCAG C^T[GA][CT]AG
SphI 551 GCATGC GCATG^C
StuI 389 AGGCCT AGG^CCT
StyI 641 CCTTGG C^C[AT][AT]GG
Tru9I 122 TTAA T^TAA
Tsp509I 374 AATT ^AATT
Tth111I 3 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 434 CCATTGTCTGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 275 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 16 enzymes) #
###################################
AcyI 52 GACGTC G[GA]^CG[CT]C
75 GGCGTC
AlwI 237 GATCC GGATC(4/5)
276 GATCC
BanII 154 GGGCCC G[GA]GC[CT]^C
244 GAGCTC
BsaHI 52 GACGTC G[GA]^CG[CT]C
75 GGCGTC
BspMI 146 ACCTGC ACCTGC(4/8)
332 GCAGGT
BsrI 118 CCAGT ACTGG(1/-1)
678 CCAGT
BstF5I 402 GGATG GGATG(2/0)
702 GGATG
DraII 153 GGGGCCC [GA]G^G[ACGT]CC[CT]
154 GGGCCCC
DsaI 59 CCACGG C^C[GA][CT]GG
546 CCGCGG
EcoO109I 153 GGGGCCC [GA]G^G[ACGT]CC[CT]
154 GGGCCCC
FokI 402 GGATG GGATG(9/13)
702 GGATG
HapII 240 CCGG C^CGG
700 CCGG
Hin1I 52 GACGTC G[GA]^CG[CT]C
75 GGCGTC
HpaII 240 CCGG C^CGG
700 CCGG
MspI 240 CCGG C^CGG
700 CCGG
TspRI 209 CACTG CA[GC]TG[ACGT][ACGT]^
327 CAGTG
#########################################
# 3 OR MORE CUT ENZYMES ( 36 enzymes) #
#########################################
AciI AluI BbvI BmyI BsaJI
BsiYI BslI BsmFI Bsp1286I BssKI
BstNI Cac8I Cfr13I DpnII EarI
EcoRII Fnu4HI HaeIII ItaI Ksp632I
MaeIII MboI MboII MnlI MslI
MspA1I MvaI MwoI NdeII NlaIII
NlaIV Sau3AI Sau96I ScrFI TseI
Tsp45I