Restriction map for
pFN21AE6220
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# NO CUT ENZYMES ( 142 enzymes) #
##################################
AatII AccII AccIII AclI AflII
AflIII AgeI AhdI Alw44I Aor51HI
ApaLI AscI AseI AsnI Asp700I
AspEI AspI AvaI AviII AvrII
BalI BbrPI BbvCI BcgI BciVI
BclI BfaI BfrI BglI BglII
BlnI BlpI Bpu1102I BsaAI BsaBI
BsaI BsaWI BseAI BsiEI BsiWI
BsmAI BsmBI BsmI BsoBI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstUI BstZ17I CelII
Cfr10I ClaI CpoI DraI DraIII
DrdI EaeI EagI Eam1105I EarI
EclXI Eco47III Eco52I EcoRV FbaI
FseI FspI HincII HindII HindIII
HpaI Ksp632I KspI MaeI MaeII
MamI MfeI MluI MluNI MroI
MscI MunI MvnI NaeI NcoI
NdeI NgoMIV NheI NotI NruI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacII SalI SapI SbfI
ScaI SexAI SfiI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI Tth111I
Van91I XbaI XcmI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 31 enzymes) #
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Acc65I 284 GGTACC G^GTACC
AccI 577 GTAGAC GT^[AC][GT]AC
Asp718I 284 GGTACC G^GTACC
AspHI 143 GAGCTC G[AT]GC[AT]^C
BamHI 14 GGATCC G^GATCC
BseRI 565 CTCCTC GAGGAG(10/8)
BsgI 701 GTGCAG GTGCAG(16/14)
BsiHKAI 143 GAGCTC G[AT]GC[AT]^C
BsmFI 137 GTCCC GGGAC(10/14)
BspMI 421 GCAGGT ACCTGC(4/8)
BstEII 529 GGTAACC G^GT[ACGT]ACC
BstPI 529 GGTAACC G^GT[ACGT]ACC
EcoO65I 529 GGTAACC G^GT[ACGT]ACC
EcoRI 445 GAATTC G^AATTC
EcoT14I 218 CCAAGG C^C[AT][AT]GG
EcoT22I 731 ATGCAT ATGCA^T
FauI 504 GCGGG CCCGC(4/6)
HgaI 670 GACGC GACGC(5/10)
KpnI 284 GGTACC GGTAC^C
MseI 524 TTAA T^TAA
MslI 790 CACAAATATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NlaIII 333 CATG CATG^
NsiI 731 ATGCAT ATGCA^T
PleI 439 GAGTC GAGTC(4/5)
PstI 620 CTGCAG CTGCA^G
SacI 143 GAGCTC GAGCT^C
StyI 218 CCAAGG C^C[AT][AT]GG
TaqI 861 TCGA T^CGA
Tru9I 524 TTAA T^TAA
Tsp45I 329 GTGAC ^GT[GC]AC
TthHB8I 861 TCGA T^CGA
###################################
# TWO CUT ENZYMES ( 42 enzymes) #
###################################
AcsI 445 GAATTC [GA]^AATT[CT]
465 GAATTT
AcyI 265 GGCGCC G[GA]^CG[CT]C
371 GGCGCC
AfaI 285 GTAC GT^AC
536 GTAC
AocI 301 CCTGAGG CC^T[ACGT]AGG
812 CCTCAGG
ApaI 213 GGGCCC GGGCC^C
311 GGGCCC
ApoI 445 GAATTC [GA]^AATT[CT]
465 GAATTT
BbeI 265 GGCGCC GGCGC^C
371 GGCGCC
BbsI 63 GAAGAC GAAGAC(2/6)
517 GAAGAC
BcnI 139 CCCGG CC^[GC]GG
246 CCGGG
BpmI 146 CTCCAG CTGGAG(16/14)
484 CTCCAG
BpuAI 63 GAAGAC GAAGAC(2/6)
517 GAAGAC
BsaHI 265 GGCGCC G[GA]^CG[CT]C
371 GGCGCC
BsrBI 399 CCGCTC CCGCTC(-3/-3)
276 GAGCGG
BstXI 95 CCAGGGAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
789 CCACAAATATGG
BstYI 3 GGATCT [GA]^GATC[CT]
14 GGATCC
Bsu36I 301 CCTGAGG CC^T[ACGT]AGG
812 CCTCAGG
CfoI 266 GCGC GCG^C
372 GCGC
DsaI 714 CCACGG C^C[GA][CT]GG
819 CCGTGG
Eco81I 301 CCTGAGG CC^T[ACGT]AGG
812 CCTCAGG
HaeII 265 GGCGCC [GA]GCGC^[CT]
371 GGCGCC
HapII 140 CCGG C^CGG
246 CCGG
HhaI 266 GCGC GCG^C
372 GCGC
Hin1I 265 GGCGCC G[GA]^CG[CT]C
371 GGCGCC
HinP1I 266 GCGC G^CGC
372 GCGC
HpaII 140 CCGG C^CGG
246 CCGG
HphI 223 GGTGA GGTGA(8/7)
571 GGTGA
KasI 265 GGCGCC G^GCGCC
371 GGCGCC
MflI 3 GGATCT [GA]^GATC[CT]
14 GGATCC
MspA1I 81 CAGCTG C[AC]G^C[GT]G
589 CAGCTG
MspI 140 CCGG C^CGG
246 CCGG
NarI 265 GGCGCC GG^CGCC
371 GGCGCC
NciI 139 CCCGG CC^[GC]GG
246 CCGGG
PpuMI 90 AGGACCC [GA]G^G[AT]CC[CT]
177 AGGTCCT
PvuII 81 CAGCTG CAG^CTG
589 CAGCTG
RsaI 285 GTAC GT^AC
536 GTAC
SfaNI 855 GATGC GCATC(5/9)
733 GCATC
SfcI 414 CTACAG C^T[GA][CT]AG
620 CTGCAG
SfoI 265 GGCGCC GGC^GCC
371 GGCGCC
SmlI 182 CTCAAG C^T[CT][GA]AG
273 CTTGAG
TfiI 628 GATTC G^A[AT]TC
682 GATTC
Tsp509I 446 AATT ^AATT
466 AATT
XhoII 3 GGATCT [GA]^GATC[CT]
14 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 43 enzymes) #
#########################################
AciI AluI AlwI AlwNI AvaII
BanI BanII BbvI BmyI BsaJI
BsiYI BslI Bsp1286I BsrI BssKI
BstF5I BstNI Cac8I Cfr13I DdeI
DpnII DraII EcoNI EcoO109I EcoRII
Fnu4HI FokI HaeIII HinfI ItaI
MaeIII MboI MboII MnlI MvaI
MwoI NdeII NlaIV Sau3AI Sau96I
ScrFI TseI TspRI