Restriction map for
pFN21AE1313
##################################
# NO CUT ENZYMES ( 165 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI Alw44I AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp718I
AspEI AvaI AviII AvrII BalI
BamHI BanI BbeI BbrPI BbvCI
BcgI BciVI BclI BfaI BfrI
BglI BlnI BsaAI BsaBI BsaHI
BsaWI BseAI BseRI BsiEI BsiWI
BsmBI BsmFI BsoBI Bsp1407I BspDI
BspEI BspLU11I BspMI BsrBI BsrDI
BsrGI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstUI BstXI BstZ17I
Bsu36I CfoI ClaI CpoI DraI
DraII DraIII DrdI DsaI EaeI
EagI Eam1105I EarI EclXI Eco47III
Eco52I Eco81I EcoNI EcoO109I EcoO65I
EcoRI EcoRV EcoT14I EcoT22I FbaI
FseI FspI HaeII HgaI HhaI
Hin1I HinP1I HincII HindII HindIII
HpaI KasI KpnI Ksp632I KspI
MaeI MaeII MamI MluI MluNI
MroI MscI MslI MvnI NarI
NcoI NdeI NheI NotI NruI
NsiI NspV PacI PaeR7I PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PstI PvuI
RsrII SacII SalI SapI SbfI
SexAI SfaNI SfcI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
StyI SwaI TaqI TthHB8I Van91I
XbaI XcmI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AfaI 205 GTAC GT^AC
AlwI 195 GGATC GGATC(4/5)
AlwNI 299 CAGCCTCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp700I 171 GAAGAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 294 GAGCTC G[AT]GC[AT]^C
AspI 380 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaII 210 GGTCC G^G[AT]CC
BbsI 79 GAAGAC GAAGAC(2/6)
BglII 266 AGATCT A^GATCT
BlpI 296 GCTCAGC GC^T[ACGT]AGC
BpmI 499 CTGGAG CTGGAG(16/14)
Bpu1102I 296 GCTCAGC GC^T[ACGT]AGC
BpuAI 79 GAAGAC GAAGAC(2/6)
BsaI 336 GGTCTC GGTCTC(1/5)
BsgI 531 CTGCAC GTGCAG(16/14)
BsiHKAI 294 GAGCTC G[AT]GC[AT]^C
BsmI 182 GCATTC GAATGC(1/-1)
BspHI 186 TCATGA T^CATGA
BsrFI 452 GCCGGC [GA]^CCGG[CT]
BstAPI 533 GCACCTCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 266 AGATCT [GA]^GATC[CT]
CelII 296 GCTCAGC GC^T[ACGT]AGC
Cfr10I 452 GCCGGC [GA]^CCGG[CT]
FauI 121 CCCGC CCCGC(4/6)
MfeI 543 CAATTG C^AATTG
MflI 266 AGATCT [GA]^GATC[CT]
MseI 163 TTAA T^TAA
MspA1I 400 CAGCTG C[AC]G^C[GT]G
MunI 543 CAATTG C^AATTG
MwoI 533 GCACCTCCTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NaeI 452 GCCGGC GCC^GGC
NgoMIV 452 GCCGGC G^CCGGC
NspI 494 ACATGC [GA]CATG^[CT]
PflFI 380 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PvuII 400 CAGCTG CAG^CTG
RcaI 186 TCATGA T^CATGA
RsaI 205 GTAC GT^AC
SacI 294 GAGCTC GAGCT^C
ScaI 204 AGTACT AGT^ACT
SmlI 291 CTTGAG C^T[CT][GA]AG
Tru9I 163 TTAA T^TAA
Tth111I 380 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoII 266 AGATCT [GA]^GATC[CT]
XmnI 171 GAAGAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
BbvI 71 GCTGC GCAGC(8/12)
430 GCTGC
BcnI 84 CCCGG CC^[GC]GG
517 CCCGG
BsiYI 5 CCTTTGTGGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
636 CCCCAGTGAGG
BslI 5 CCTTTGTGGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
636 CCCCAGTGAGG
BsmAI 337 GTCTC GTCTC(1/5)
356 GTCTC
DpnII 196 GATC ^GATC
267 GATC
HaeIII 109 GGCC GG^CC
455 GGCC
MaeIII 365 GTGAC ^GT[ACGT]AC
565 GTGAC
MboI 196 GATC ^GATC
267 GATC
NciI 84 CCCGG CC^[GC]GG
517 CCCGG
NdeII 196 GATC ^GATC
267 GATC
NlaIV 102 GGAACC GG[ACGT]^[ACGT]CC
484 GGCTCC
PleI 217 GACTC GAGTC(4/5)
502 GAGTC
Sau3AI 196 GATC ^GATC
267 GATC
TseI 71 GCTGC G^C[AT]GC
430 GCTGC
Tsp45I 365 GTGAC ^GT[GC]AC
565 GTGAC
TspRI 67 CAGTG CA[GC]TG[ACGT][ACGT]^
639 CAGTG
#########################################
# 3 OR MORE CUT ENZYMES ( 32 enzymes) #
#########################################
AciI AcsI AluI ApoI BanII
BmyI BsaJI Bsp1286I BsrI BssKI
BstF5I BstNI Cac8I Cfr13I DdeI
EcoRII Fnu4HI FokI HapII HinfI
HpaII HphI ItaI MboII MnlI
MspI MvaI NlaIII Sau96I ScrFI
TfiI Tsp509I