Restriction map for
pFN21AE4557
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# NO CUT ENZYMES ( 114 enzymes) #
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AatII AccII AclI AflIII AgeI
AhdI Alw44I AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp700I
AspEI AspI AvaI AviII AvrII
BamHI BbeI BbrPI BbvCI BcgI
BciVI BclI BcnI BglII BlnI
BlpI Bpu1102I BsaAI BsaI BsiEI
BsiWI BsoBI BspDI BspHI BspLU11I
BspMI BsrBI BssHII BstAPI BstEII
BstPI BstUI BstXI Bsu36I CelII
ClaI CpoI DraI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoO65I
EcoRV EcoT22I FauI FbaI FseI
FspI HaeII HindIII HpaI KasI
KspI MfeI MluI MunI MvnI
NarI NciI NdeI NotI NruI
NsiI PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PpuMI PshBI
Psp1406I PvuI RcaI RsrII SacI
SacII SalI SapI SbfI SexAI
SfiI SfoI SgrAI SmaI SnaBI
SpeI Sse8387I StuI SwaI Tth111I
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 54 enzymes) #
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Acc65I 688 GGTACC G^GTACC
AccI 642 GTATAC GT^[AC][GT]AC
AccIII 254 TCCGGA T^CCGGA
AcyI 1931 GGCGTC G[GA]^CG[CT]C
AflII 1233 CTTAAG C^TTAAG
Asp718I 688 GGTACC G^GTACC
AspHI 650 GAGCAC G[AT]GC[AT]^C
BalI 1119 TGGCCA TGG^CCA
BanI 688 GGTACC G^G[CT][GA]CC
BbsI 838 GAAGAC GAAGAC(2/6)
BfrI 1233 CTTAAG C^TTAAG
BglI 1999 GCCATCATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 838 GAAGAC GAAGAC(2/6)
BsaBI 69 GATGCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 1931 GGCGTC G[GA]^CG[CT]C
BseAI 254 TCCGGA T^CCGGA
BsiHKAI 650 GAGCAC G[AT]GC[AT]^C
BsmBI 1933 CGTCTC CGTCTC(1/5)
BspEI 254 TCCGGA T^CCGGA
BsrFI 310 GCCGGC [GA]^CCGG[CT]
Bst1107I 642 GTATAC GTA^TAC
BstBI 1376 TTCGAA TT^CGAA
BstZ17I 642 GTATAC GTA^TAC
CfoI 1239 GCGC GCG^C
Cfr10I 310 GCCGGC [GA]^CCGG[CT]
DraII 903 AGGGCCT [GA]G^G[ACGT]CC[CT]
DraIII 1135 CACACCGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1441 GACATGAGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 552 CCTCCATAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 903 AGGGCCT [GA]G^G[ACGT]CC[CT]
EcoRI 1373 GAATTC G^AATTC
HgaI 1932 GCGTC GACGC(5/10)
HhaI 1239 GCGC GCG^C
Hin1I 1931 GGCGTC G[GA]^CG[CT]C
HinP1I 1239 GCGC G^CGC
HincII 1924 GTTGAC GT[CT]^[GA]AC
HindII 1924 GTTGAC GT[CT]^[GA]AC
KpnI 688 GGTACC GGTAC^C
MamI 69 GATGCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 1119 TGGCCA TGG^CCA
MroI 254 TCCGGA T^CCGGA
MscI 1119 TGGCCA TGG^CCA
NaeI 310 GCCGGC GCC^GGC
NgoMIV 310 GCCGGC G^CCGGC
NheI 196 GCTAGC G^CTAGC
NspI 1280 GCATGC [GA]CATG^[CT]
NspV 1376 TTCGAA TT^CGAA
PshAI 1927 GACTGGCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 1552 CTGCAG CTGCA^G
ScaI 19 AGTACT AGT^ACT
SfuI 1376 TTCGAA TT^CGAA
SphI 1280 GCATGC GCATG^C
SspI 1843 AATATT AAT^ATT
XbaI 1497 TCTAGA T^CTAGA
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# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AciI 35 CCGC CCGC(-3/-1)
38 CCGC
AvaII 46 GGTCC G^G[AT]CC
1910 GGTCC
BbvI 28 GCTGC GCAGC(8/12)
1996 GCTGC
BseRI 1537 CTCCTC GAGGAG(10/8)
937 GAGGAG
BsmI 106 GCATTC GAATGC(1/-1)
544 GCATTC
Bsp1407I 281 TGTACA T^GTACA
1160 TGTACA
BsrGI 281 TGTACA T^GTACA
1160 TGTACA
BssSI 352 CTCGTG CACGAG(-5/-1)
424 CTCGTG
EaeI 308 TGGCCG [CT]^GGCC[GA]
1119 TGGCCA
MaeII 272 ACGT A^CGT
1601 ACGT
MseI 811 TTAA T^TAA
1234 TTAA
MspA1I 26 CAGCTG C[AC]G^C[GT]G
149 CAGCTG
PflMI 924 CCAGGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1752 CCAGACCATGG
PvuII 26 CAGCTG CAG^CTG
149 CAGCTG
SfcI 23 CTACAG C^T[GA][CT]AG
1552 CTGCAG
SmlI 293 CTCAAG C^T[CT][GA]AG
1233 CTTAAG
SspBI 281 TGTACA T^GTACA
1160 TGTACA
TaqI 289 TCGA T^CGA
1377 TCGA
TfiI 1150 GATTC G^A[AT]TC
1291 GATTC
Tru9I 811 TTAA T^TAA
1234 TTAA
TseI 28 GCTGC G^C[AT]GC
1996 GCTGC
TthHB8I 289 TCGA T^CGA
1377 TCGA
Van91I 924 CCAGGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1752 CCAGACCATGG
XcmI 390 CCATTGTATTGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1613 CCATTGGGCCTCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 66 enzymes) #
#########################################
AcsI AfaI AluI AlwI AlwNI
ApoI BanII BfaI BmyI BpmI
BsaJI BsaWI BsgI BsiYI BslI
BsmAI BsmFI Bsp1286I BsrDI BsrI
BssKI BstF5I BstNI BstYI Cac8I
Cfr13I DdeI DpnII DsaI EarI
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeIII MboI MboII
MflI MnlI MslI MspI MvaI
MwoI NcoI NdeII NlaIII NlaIV
PleI RsaI Sau3AI Sau96I ScrFI
SfaNI StyI Tsp45I Tsp509I TspRI
XhoII