Restriction map for
pFN21AE6112
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# NO CUT ENZYMES ( 194 enzymes) #
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AatII Acc65I AccI AccII AccIII
AciI AclI AcsI AflII AflIII
AgeI AhdI Alw44I AocI Aor51HI
ApaI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AvaI AvaII AviII AvrII BamHI
BanII BbrPI BbsI BbvCI BcgI
BciVI BclI BcnI BfrI BglI
BglII BlnI BlpI BpmI Bpu1102I
BpuAI BsaAI BsaBI BsaI BsaWI
BseAI BseRI BsgI BsiEI BsiWI
BsmAI BsmBI BsmFI BsmI BsoBI
Bsp1407I BspDI BspEI BspLU11I BspMI
BsrBI BsrFI BsrGI BsrI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstXI BstZ17I Bsu36I
Cac8I CelII Cfr10I Cfr13I ClaI
CpoI DraI DraII DraIII DrdI
DsaI EagI Eam1105I EarI EclXI
Eco47III Eco52I Eco81I EcoNI EcoO109I
EcoO65I EcoRI EcoRV EcoT14I EcoT22I
FauI FbaI FseI FspI HapII
HgaI HincII HindII HindIII HinfI
HpaI HpaII KpnI Ksp632I KspI
MaeII MamI MfeI MluI MroI
MseI MslI MspI MunI MvnI
MwoI NaeI NciI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PleI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PstI PvuI RsrII SacI SacII
SalI SapI Sau96I SbfI ScaI
SexAI SfcI SfiI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI StyI
SwaI TaqI TfiI Tru9I TspRI
Tth111I TthHB8I Van91I XbaI XcmI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AcyI 32 GGCGCC G[GA]^CG[CT]C
AlwI 386 GGATC GGATC(4/5)
AlwNI 13 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
AspHI 171 GAGCAC G[AT]GC[AT]^C
BanI 32 GGCGCC G^G[CT][GA]CC
BbeI 32 GGCGCC GGCGC^C
BbvI 15 GCAGC GCAGC(8/12)
BfaI 76 CTAG C^TAG
BmyI 171 GAGCAC G[AGT]GC[ACT]^C
BsaHI 32 GGCGCC G[GA]^CG[CT]C
BsiHKAI 171 GAGCAC G[AT]GC[AT]^C
BsiYI 377 CCTGTACTCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 377 CCTGTACTCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
Bsp1286I 171 GAGCAC G[AGT]GC[ACT]^C
BspHI 362 TCATGA T^CATGA
BsrDI 109 GCAATG GCAATG(2/0)
BstF5I 300 GGATG GGATG(2/0)
BstYI 386 GGATCT [GA]^GATC[CT]
DpnII 387 GATC ^GATC
Fnu4HI 15 GCAGC GC^[ACGT]GC
FokI 300 GGATG GGATG(9/13)
HaeII 32 GGCGCC [GA]GCGC^[CT]
Hin1I 32 GGCGCC G[GA]^CG[CT]C
ItaI 15 GCAGC GC^[ACGT]GC
KasI 32 GGCGCC G^GCGCC
MaeI 76 CTAG C^TAG
MaeIII 373 GTCAC ^GT[ACGT]AC
MboI 387 GATC ^GATC
MflI 386 GGATCT [GA]^GATC[CT]
MnlI 153 CCTC CCTC(7/6)
NarI 32 GGCGCC GG^CGCC
NdeII 387 GATC ^GATC
NlaIV 32 GGCGCC GG[ACGT]^[ACGT]CC
RcaI 362 TCATGA T^CATGA
Sau3AI 387 GATC ^GATC
SfaNI 288 GCATC GCATC(5/9)
SfoI 32 GGCGCC GGC^GCC
TseI 15 GCAGC G^C[AT]GC
Tsp45I 373 GTCAC ^GT[GC]AC
XhoII 386 GGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AfaI 200 GTAC GT^AC
380 GTAC
BalI 2 TGGCCA TGG^CCA
113 TGGCCA
BsaJI 203 CCCTGG C^C[ACGT][ACGT]GG
204 CCTGGG
BssKI 36 CCTGG ^CC[ACGT]GG
204 CCTGG
BstNI 36 CCTGG CC^[AT]GG
204 CCTGG
CfoI 33 GCGC GCG^C
107 GCGC
DdeI 167 CTGAG C^T[ACGT]AG
248 CTCAG
EaeI 2 TGGCCA [CT]^GGCC[GA]
113 TGGCCA
EcoRII 36 CCTGG ^CC[AT]GG
204 CCTGG
HaeIII 3 GGCC GG^CC
114 GGCC
HhaI 33 GCGC GCG^C
107 GCGC
HinP1I 33 GCGC G^CGC
107 GCGC
HphI 155 TCACC GGTGA(8/7)
374 TCACC
MboII 26 GAAGA GAAGA(8/7)
220 GAAGA
MluNI 2 TGGCCA TGG^CCA
113 TGGCCA
MscI 2 TGGCCA TGG^CCA
113 TGGCCA
MspA1I 16 CAGCTG C[AC]G^C[GT]G
230 CAGCTG
MvaI 36 CCTGG CC^[AT]GG
204 CCTGG
NlaIII 66 CATG CATG^
363 CATG
PvuII 16 CAGCTG CAG^CTG
230 CAGCTG
RsaI 200 GTAC GT^AC
380 GTAC
ScrFI 36 CCTGG CC^[ACGT]GG
204 CCTGG
Tsp509I 329 AATT ^AATT
344 AATT
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# 3 OR MORE CUT ENZYMES ( 1 enzymes) #
#########################################
AluI