Restriction map for
pFN21AE6421
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# NO CUT ENZYMES ( 80 enzymes) #
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AatII AclI AflII AscI AseI
AsnI Asp700I AvrII BamHI BbsI
BbvCI BciVI BclI BfrI BglII
BlnI BpuAI BsaBI BsmBI BsmI
Bsp1407I BspDI BspHI BspLU11I BsrGI
BstAPI BstBI BstEII BstPI ClaI
DraI DrdI EcoO65I EcoRV EcoT22I
FbaI FseI HincII HindII HindIII
HpaI MamI MfeI MluI MseI
MunI NaeI NdeI NgoMIV NheI
NruI NsiI NspV PacI PaeR7I
PmeI PpuMI PshBI Psp1406I PvuI
RcaI SalI SbfI ScaI SexAI
SfiI SfuI SmaI SnaBI SphI
Sse8387I SspBI SspI SwaI Tru9I
XbaI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 64 enzymes) #
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Acc65I 1599 GGTACC G^GTACC
AccIII 149 TCCGGA T^CCGGA
AcsI 544 GAATTC [GA]^AATT[CT]
Alw44I 121 GTGCAC G^TGCAC
AlwI 618 GATCC GGATC(4/5)
AocI 1467 CCTCAGG CC^T[ACGT]AGG
ApaI 1534 GGGCCC GGGCC^C
ApaLI 121 GTGCAC G^TGCAC
ApoI 544 GAATTC [GA]^AATT[CT]
Asp718I 1599 GGTACC G^GTACC
BbrPI 1245 CACGTG CAC^GTG
BcgI 1448 GCAGCTGCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 58 GCCATCGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 1377 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1377 GCTCAGC GC^T[ACGT]AGC
BsaI 833 GGTCTC GGTCTC(1/5)
BseAI 149 TCCGGA T^CCGGA
BseRI 1651 GAGGAG GAGGAG(10/8)
BsiEI 273 CGGCCG CG[GA][CT]^CG
BsiWI 1160 CGTACG C^GTACG
BspEI 149 TCCGGA T^CCGGA
BsrBI 1047 CCGCTC CCGCTC(-3/-3)
BsrDI 1037 CATTGC GCAATG(2/0)
BssSI 403 CTCGTG CACGAG(-5/-1)
Bst1107I 1194 GTATAC GTA^TAC
BstYI 617 AGATCC [GA]^GATC[CT]
BstZ17I 1194 GTATAC GTA^TAC
Bsu36I 1467 CCTCAGG CC^T[ACGT]AGG
CelII 1377 GCTCAGC GC^T[ACGT]AGC
CpoI 151 CGGACCG CG^G[AT]CCG
DpnII 618 GATC ^GATC
EagI 273 CGGCCG C^GGCCG
EclXI 273 CGGCCG C^GGCCG
Eco52I 273 CGGCCG C^GGCCG
Eco81I 1467 CCTCAGG CC^T[ACGT]AGG
EcoRI 544 GAATTC G^AATTC
EcoT14I 383 CCATGG C^C[AT][AT]GG
KpnI 1599 GGTACC GGTAC^C
KspI 227 CCGCGG CCGC^GG
MboI 618 GATC ^GATC
MflI 617 AGATCC [GA]^GATC[CT]
MroI 149 TCCGGA T^CCGGA
NcoI 383 CCATGG C^CATGG
NdeII 618 GATC ^GATC
NotI 272 GCGGCCGC GC^GGCCGC
NspI 1502 ACATGC [GA]CATG^[CT]
PflMI 1134 CCACTGCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 1245 CACGTG CAC^GTG
PmlI 1245 CACGTG CAC^GTG
PstI 481 CTGCAG CTGCA^G
RsrII 151 CGGACCG CG^G[AT]CCG
SacII 227 CCGCGG CCGC^GG
Sau3AI 618 GATC ^GATC
SfcI 481 CTGCAG C^T[GA][CT]AG
SgrAI 1623 CACCGGTG C[GA]^CCGG[CT]G
SpeI 1401 ACTAGT A^CTAGT
StuI 717 AGGCCT AGG^CCT
StyI 383 CCATGG C^C[AT][AT]GG
TfiI 711 GATTC G^A[AT]TC
Tsp45I 1169 GTGAC ^GT[GC]AC
Tsp509I 545 AATT ^AATT
Van91I 1134 CCACTGCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1064 CCAAGAGCAACGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 617 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 24 enzymes) #
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AccI 43 GTCTAC GT^[AC][GT]AC
1194 GTATAC
AflIII 1244 ACACGT A^C[GA][CT]GT
1246 ACGTGT
AgeI 1596 ACCGGT A^CCGGT
1624 ACCGGT
AlwNI 604 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
1470 CAGGGCCTG
Aor51HI 695 AGCGCT AGC^GCT
1290 AGCGCT
AviII 600 TGCGCA TGC^GCA
746 TGCGCA
BalI 236 TGGCCA TGG^CCA
797 TGGCCA
BsaAI 1162 TACGTG [CT]AC^GT[GA]
1245 CACGTG
BsmAI 635 GAGAC GTCTC(1/5)
834 GTCTC
BspMI 740 ACCTGC ACCTGC(4/8)
162 GCAGGT
BsrFI 1596 ACCGGT [GA]^CCGG[CT]
1624 ACCGGT
Cfr10I 1596 ACCGGT [GA]^CCGG[CT]
1624 ACCGGT
DraIII 1135 CACTGCGTG CAC[ACGT][ACGT][ACGT]^GTG
1360 CACCCCGTG
Eco47III 695 AGCGCT AGC^GCT
1290 AGCGCT
EcoNI 438 CCTGGCCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1386 CCTCTTCAAGG
FspI 600 TGCGCA TGC^GCA
746 TGCGCA
MaeIII 992 GTTAC ^GT[ACGT]AC
1169 GTGAC
MluNI 236 TGGCCA TGG^CCA
797 TGGCCA
MscI 236 TGGCCA TGG^CCA
797 TGGCCA
PinAI 1596 ACCGGT A^CCGGT
1624 ACCGGT
PleI 306 GACTC GAGTC(4/5)
1255 GACTC
PshAI 87 GACGCGCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
924 GACCAAAGTC
PvuII 607 CAGCTG CAG^CTG
1449 CAGCTG
SapI 1123 GAAGAGC GCTCTTC(1/4)
786 GCTCTTC
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# 3 OR MORE CUT ENZYMES ( 90 enzymes) #
#########################################
AccII AciI AcyI AfaI AhdI
AluI AspEI AspHI AspI AvaI
AvaII BanI BanII BbeI BbvI
BcnI BfaI BmyI BpmI BsaHI
BsaJI BsaWI BsgI BsiHKAI BsiYI
BslI BsmFI BsoBI Bsp1286I BsrI
BssHII BssKI BstF5I BstNI BstUI
BstXI Cac8I CfoI Cfr13I DdeI
DraII DsaI EaeI Eam1105I EarI
EcoO109I EcoRII FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI KasI Ksp632I MaeI MaeII
MboII MnlI MslI MspA1I MspI
MvaI MvnI MwoI NarI NciI
NlaIII NlaIV PflFI RsaI SacI
Sau96I ScrFI SfaNI SfoI SmlI
TaqI TseI TspRI Tth111I TthHB8I