Restriction map for
pFN21AE4505
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# NO CUT ENZYMES ( 120 enzymes) #
##################################
AatII AccI AccIII AcsI AcyI
AflII AgeI AhdI Alw44I AocI
ApaLI ApoI AscI AseI AsnI
AspEI AvaI AviII AvrII BamHI
BbeI BbrPI BcgI BclI BfrI
BglI BglII BlnI BlpI Bpu1102I
BsaHI BsaI BsaWI BseAI BsgI
BsiWI BsmI BsoBI Bsp1407I BspDI
BspEI BspLU11I BsrBI BsrDI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstXI BstZ17I Bsu36I CelII ClaI
CpoI DraI DraIII DrdI DsaI
Eam1105I Eco81I EcoRI EcoRV EcoT22I
FbaI FseI FspI Hin1I HindIII
HpaI KasI KspI MfeI MluI
MroI MseI MunI NarI NcoI
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PinAI PmaCI
PmeI PmlI PshAI PshBI PstI
PvuI RsrII SacII SalI SapI
SbfI ScaI SfiI SfoI SfuI
SgrAI SmaI SmlI SpeI SphI
Sse8387I SspBI SspI SwaI Tru9I
XbaI XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AccII 159 CGCG CG^CG
AclI 1493 AACGTT AA^CGTT
AflIII 596 ACGTGT A^C[GA][CT]GT
ApaI 1238 GGGCCC GGGCC^C
AspI 1081 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 780 TGGCCA TGG^CCA
BbsI 931 GTCTTC GAAGAC(2/6)
BfaI 551 CTAG C^TAG
BpuAI 931 GTCTTC GAAGAC(2/6)
BsaAI 1303 TACGTA [CT]AC^GT[GA]
BsaBI 940 GATTTTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI 155 CGGCCG CG[GA][CT]^CG
BsmBI 1086 CGTCTC CGTCTC(1/5)
BsrFI 1228 GCCGGC [GA]^CCGG[CT]
BstEII 1218 GGTCACC G^GT[ACGT]ACC
BstPI 1218 GGTCACC G^GT[ACGT]ACC
BstUI 159 CGCG CG^CG
BstYI 1001 AGATCC [GA]^GATC[CT]
Cfr10I 1228 GCCGGC [GA]^CCGG[CT]
EagI 155 CGGCCG C^GGCCG
EarI 1089 CTCTTC CTCTTC(1/4)
EclXI 155 CGGCCG C^GGCCG
Eco52I 155 CGGCCG C^GGCCG
EcoNI 180 CCTCGACGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1218 GGTCACC G^GT[ACGT]ACC
EcoT14I 1223 CCAAGG C^C[AT][AT]GG
FauI 494 CCCGC CCCGC(4/6)
HgaI 1071 GACGC GACGC(5/10)
HincII 1464 GTTGAC GT[CT]^[GA]AC
HindII 1464 GTTGAC GT[CT]^[GA]AC
Ksp632I 1089 CTCTTC CTCTTC(1/4)
MaeI 551 CTAG C^TAG
MamI 940 GATTTTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 1001 AGATCC [GA]^GATC[CT]
MluNI 780 TGGCCA TGG^CCA
MscI 780 TGGCCA TGG^CCA
MvnI 159 CGCG CG^CG
NaeI 1228 GCCGGC GCC^GGC
NdeI 635 CATATG CA^TATG
NgoMIV 1228 GCCGGC G^CCGGC
PflFI 1081 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 673 GGGTCCT [GA]G^G[AT]CC[CT]
Psp1406I 1493 AACGTT AA^CGTT
PvuII 1048 CAGCTG CAG^CTG
SfcI 293 CTGTAG C^T[GA][CT]AG
SnaBI 1303 TACGTA TAC^GTA
StuI 188 AGGCCT AGG^CCT
StyI 1223 CCAAGG C^C[AT][AT]GG
TaqI 182 TCGA T^CGA
Tth111I 1081 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 182 TCGA T^CGA
XhoII 1001 AGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
Acc65I 1259 GGTACC G^GTACC
1330 GGTACC
AlwI 622 GATCC GGATC(4/5)
1002 GATCC
Aor51HI 104 AGCGCT AGC^GCT
197 AGCGCT
Asp718I 1259 GGTACC G^GTACC
1330 GGTACC
BbvCI 448 GCTGAGG CCTCAGC(-5/-2)
1521 GCTGAGG
BseRI 379 CTCCTC GAGGAG(10/8)
959 GAGGAG
BsmFI 798 GGGAC GGGAC(10/14)
1408 GTCCC
BspHI 662 TCATGA T^CATGA
899 TCATGA
DpnII 622 GATC ^GATC
1002 GATC
DraII 673 GGGTCCT [GA]G^G[ACGT]CC[CT]
1238 GGGCCCT
EaeI 155 CGGCCG [CT]^GGCC[GA]
780 TGGCCA
Eco47III 104 AGCGCT AGC^GCT
197 AGCGCT
EcoO109I 673 GGGTCCT [GA]G^G[ACGT]CC[CT]
1238 GGGCCCT
HaeII 104 AGCGCT [GA]GCGC^[CT]
197 AGCGCT
KpnI 1259 GGTACC GGTAC^C
1330 GGTACC
MboI 622 GATC ^GATC
1002 GATC
MslI 302 CACCTGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1164 CATACAGGTG
MspA1I 1048 CAGCTG C[AC]G^C[GT]G
1509 CCGCTG
MwoI 40 GCAGCGTTCGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
49 GCAGGACGGGC
NdeII 622 GATC ^GATC
1002 GATC
PflMI 11 CCAGGCTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1412 CCAGGCTCTGG
RcaI 662 TCATGA T^CATGA
899 TCATGA
SacI 401 GAGCTC GAGCT^C
658 GAGCTC
Sau3AI 622 GATC ^GATC
1002 GATC
SexAI 801 ACCAGGT A^CC[AT]GGT
1403 ACCTGGT
SfaNI 554 GCATC GCATC(5/9)
609 GCATC
TfiI 870 GAATC G^A[AT]TC
1146 GATTC
Van91I 11 CCAGGCTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1412 CCAGGCTCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 58 enzymes) #
#########################################
AciI AfaI AluI AlwNI Asp700I
AspHI AvaII BanI BanII BbvI
BciVI BcnI BmyI BpmI BsaJI
BsiHKAI BsiYI BslI BsmAI Bsp1286I
BspMI BsrI BssKI BstF5I BstNI
Cac8I CfoI Cfr13I DdeI EcoRII
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
MaeII MaeIII MboII MnlI MspI
MvaI NciI NlaIII NlaIV PleI
RsaI Sau96I ScrFI TseI Tsp45I
Tsp509I TspRI XmnI